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- W1499591878 abstract "A new public high-amylose maize line, GEMS-0067, has been developed by USDA-ARS Germplasm Enhancement of Maize (GEM) Project. GEMS-0067 maize line is a homozygous mutant of amylose-extender (ae) gene and high-amylose modifier (HAM) gene(s). GEMS0067 starches consist of 83.1-85.6% apparent amylose and 39.4%-43.2% resistant starch (RS), which are larger than the ae single-mutant starches (61.7-67.7% and 11.5%-19.1%, respectively) and normal maize starch (~30% and <1%, respectively). Maize ae-mutant starches consisted of spherical and elongated granules (up to 32%), which differed from normal maize starch, consisting of spherical and angular granules. The objectives of this study were to understand the mechanism of the RS formation in the maize ae-mutant starches, the crystalline structures of the maize ae-mutant starches, the formation of RS and elongated starch granules during the kernel development, and how dosage of the HAM gene(s) affected the structures and properties of endosperm starch of maize ae-mutant. We analyzed the structures and properties of the RS residues that remained after enzymatic hydrolysis of the maize ae-mutant starches at 95-100 C (AOAC method 991.43). The RS residues consisted of mainly partially hydrolyzed amylose and intermediate component (IC) and were more concentrated in the elongated starch granules and the outer layer of the spherical granules. The RS residues displayed the B-type polymorph and had gelatinization temperatures above 100 C. These results suggested that long-chain double-helical crystallites of amylose/IC were present in the native maize ae-mutant starches. The amylose/IC crystallites maintained the semi-crystalline structures at 95-100 C and were resistant to enzymatic hydrolysis at 95-" @default.
- W1499591878 created "2016-06-24" @default.
- W1499591878 creator A5091014403 @default.
- W1499591878 date "2018-08-10" @default.
- W1499591878 modified "2023-10-16" @default.
- W1499591878 title "Resistant-starch formation in high-amylose maize starch" @default.
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