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- W1499839009 abstract "This chapter discusses methods and strategies that proved useful in improving both the sophistication and the efficiency of voltage-clamp experiments. It concentrates here on the analysis of data that are strongly affected by various experimental limitations and that require special software tools. It discusses the way ion channel kinetics are simulated using rate models and how these models are fit to experimental data sets. In particular, it stresses the way such an analysis task is facilitated when corresponding software tools are integrated into a modular program package that also supports data acquisition. This chapter addresses several questions that arise particularly when data of nonideal voltage-clamp experiments are to be analyzed. Among them are questions on leak correction as well as bandwidth and rise time limitations. These topics are discussed using two-electrode voltage-clamp (TEV) ionic current and gating current data from potassium channels expressed in Xenopus oocytes. It also discusses how large kinetic models can be fit to heterogeneous data sets and how data simulation is used to test results arising from nonstationary noise analyses." @default.
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- W1499839009 date "1998-01-01" @default.
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- W1499839009 title "[23] Voltage-dependent ion channels: Analysis of nonideal macroscopic current data" @default.
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- W1499839009 doi "https://doi.org/10.1016/s0076-6879(98)93026-9" @default.
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