FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1499891289> ?p ?o ?g. }

• W1499891289 endingPage "1500" @default.
• W1499891289 startingPage "1493" @default.
• W1499891289 abstract "We describe functional tests and molecular modeling of erythroid Kruppel-like factor (EKLF) interactions with its DNA binding site. EKLF, a zinc finger-containing, erythroid-specific transcription factor, binds and transactivates from the CACCC element, an evolutionarily conserved DNA sequence present within a large number of erythroid-specific promoters and enhancers. This DNA binding element is the site of naturally occurring point mutations that give rise to beta-thalassemia. We have directly tested whether CAC site point mutations (including two of the beta-thalassemia mutants) affect EKLF transactivation and DNA binding function. In vivo analyses demonstrate that EKLF is unable to transactivate a reporter plasmid that contains these mutations. In vitro analyses reveal a 40-100-fold decrease in binding affinity for these sites that accounts for the in vivo observations. The homology between the three EKLF and Zif268 zinc fingers and their conserved sequence-specific contacts to their target site allowed us to formulate a molecular model of the EKLF/CAC site complex, based primarily on energy minimization/refinement of the Zif268/DNA co-crystal structure. These models suggest that both specific and nonspecific hydrogen bonding play a critical role in the ability of EKLF to prefer binding to its cognate site. Analysis of sequence-specific contacts by EKLF to its target site within the beta-globin promoter verified the residues predicted to be important by the functional and modeling data. Together these results demonstrate that EKLF displays a strong discriminatory ability among potential DNA target sites consistent with the beta-thalassemia data. They also suggest that lack of EKLF binding to these sites may play a determining role in its phenotype, and they strengthen the evidence in favor of EKLF's proposed role in erythroid-specific transcriptional activation through the CACCC elements." @default.
• W1499891289 created "2016-06-24" @default.
• W1499891289 creator A5002966230 @default.
• W1499891289 creator A5011099905 @default.
• W1499891289 creator A5048608409 @default.
• W1499891289 date "1994-01-01" @default.
• W1499891289 modified "2023-10-10" @default.
• W1499891289 title "Analyses of beta-thalassemia mutant DNA interactions with erythroid Krüppel-like factor (EKLF), an erythroid cell-specific transcription factor." @default.
• W1499891289 cites W1505715400 @default.
• W1499891289 cites W1514016855 @default.
• W1499891289 cites W1531234455 @default.
• W1499891289 cites W1540713407 @default.
• W1499891289 cites W1545971708 @default.
• W1499891289 cites W1558516755 @default.
• W1499891289 cites W1969760979 @default.
• W1499891289 cites W1970865088 @default.
• W1499891289 cites W1971251199 @default.
• W1499891289 cites W1976459530 @default.
• W1499891289 cites W1978713358 @default.
• W1499891289 cites W1980645183 @default.
• W1499891289 cites W1982695907 @default.
• W1499891289 cites W1985971672 @default.
• W1499891289 cites W1987498682 @default.
• W1499891289 cites W1990045705 @default.
• W1499891289 cites W2000559841 @default.
• W1499891289 cites W2001772277 @default.
• W1499891289 cites W2007327608 @default.
• W1499891289 cites W2008857546 @default.
• W1499891289 cites W2009821284 @default.
• W1499891289 cites W2017202336 @default.
• W1499891289 cites W2021388708 @default.
• W1499891289 cites W2023374624 @default.
• W1499891289 cites W2023557705 @default.
• W1499891289 cites W2025847301 @default.
• W1499891289 cites W2028553942 @default.
• W1499891289 cites W2030333654 @default.
• W1499891289 cites W2039422399 @default.
• W1499891289 cites W2041105544 @default.
• W1499891289 cites W2044089537 @default.
• W1499891289 cites W2045284924 @default.
• W1499891289 cites W2046969721 @default.
• W1499891289 cites W2051520229 @default.
• W1499891289 cites W2052807493 @default.
• W1499891289 cites W2056326002 @default.
• W1499891289 cites W2083517254 @default.
• W1499891289 cites W2086548325 @default.
• W1499891289 cites W2089019735 @default.
• W1499891289 cites W2095394228 @default.
• W1499891289 cites W2100105710 @default.
• W1499891289 cites W2101141723 @default.
• W1499891289 cites W2119692824 @default.
• W1499891289 cites W2124100270 @default.
• W1499891289 cites W2132291850 @default.
• W1499891289 cites W2133335149 @default.
• W1499891289 cites W2142342476 @default.
• W1499891289 cites W2144177887 @default.
• W1499891289 cites W2145619991 @default.
• W1499891289 cites W2169578753 @default.
• W1499891289 cites W286297709 @default.
• W1499891289 cites W4234174538 @default.
• W1499891289 doi "https://doi.org/10.1016/s0021-9258(17)42283-6" @default.
• W1499891289 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8288615" @default.
• W1499891289 hasPublicationYear "1994" @default.
• W1499891289 type Work @default.
• W1499891289 sameAs 1499891289 @default.
• W1499891289 citedByCount "183" @default.
• W1499891289 countsByYear W14998912892012 @default.
• W1499891289 countsByYear W14998912892013 @default.
• W1499891289 countsByYear W14998912892014 @default.
• W1499891289 countsByYear W14998912892015 @default.
• W1499891289 countsByYear W14998912892016 @default.
• W1499891289 countsByYear W14998912892017 @default.
• W1499891289 countsByYear W14998912892018 @default.
• W1499891289 countsByYear W14998912892019 @default.
• W1499891289 countsByYear W14998912892020 @default.
• W1499891289 countsByYear W14998912892021 @default.
• W1499891289 countsByYear W14998912892022 @default.
• W1499891289 countsByYear W14998912892023 @default.
• W1499891289 crossrefType "journal-article" @default.
• W1499891289 hasAuthorship W1499891289A5002966230 @default.
• W1499891289 hasAuthorship W1499891289A5011099905 @default.
• W1499891289 hasAuthorship W1499891289A5048608409 @default.
• W1499891289 hasBestOaLocation W14998912891 @default.
• W1499891289 hasConcept C104317684 @default.
• W1499891289 hasConcept C138885662 @default.
• W1499891289 hasConcept C143065580 @default.
• W1499891289 hasConcept C153911025 @default.
• W1499891289 hasConcept C179926584 @default.
• W1499891289 hasConcept C192196715 @default.
• W1499891289 hasConcept C41895202 @default.
• W1499891289 hasConcept C54355233 @default.
• W1499891289 hasConcept C552990157 @default.
• W1499891289 hasConcept C86339819 @default.
• W1499891289 hasConcept C86803240 @default.
• W1499891289 hasConceptScore W1499891289C104317684 @default.
• W1499891289 hasConceptScore W1499891289C138885662 @default.
• W1499891289 hasConceptScore W1499891289C143065580 @default.
• W1499891289 hasConceptScore W1499891289C153911025 @default.

• next