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- W1500983825 abstract "Kilham rat virus (KRV) was grown in a rat nephroma cell line and was purified by two isopycnic centrifugations in cesium chloride. The virus contains single-stranded deoxyribonucleic acid (DNA) with a molecular weight of approximately 1.6 x 10(6). The DNA was extracted from the virion by both phenol extraction and by 2% sodium dodecyl sulfate at 50 C. KRV DNA, extracted by both procedures, was observed in an electron microscope by using a cytochrome c or diethylaminoethyldextran monolayer. The DNA was also exposed to exonuclease I, an enzyme which hydrolyzes specifically linear, single-stranded DNA. Hydrolysis of 70 to 80% of the DNA was observed. Both the enzymatic and the electron microscope studies support the conclusion that extracted KRV DNA is a single-stranded, linear molecule. The length of the DNA was measured in the electron microscope and determined to be 1.505 +/- 0.206 mum." @default.
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- W1500983825 date "1971-06-01" @default.
- W1500983825 modified "2023-10-16" @default.
- W1500983825 title "Linear, Single-Stranded Deoxyribonucleic Acid Isolated from Kilham Rat Virus" @default.
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- W1500983825 doi "https://doi.org/10.1128/jvi.7.6.830-835.1971" @default.
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