Matches in SemOpenAlex for { <https://semopenalex.org/work/W1501059600> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W1501059600 abstract "Selective marking of a single cell within a living embryo is often difficult due to the inaccuracy and invasiveness of standard techniques. This unit describes a minimally invasive optical protocol that uses 405-nm laser light to photoactivate a variant of green fluorescent protein (PAGFP). This method takes advantage of the accessibility of the chick embryo to inject PAGFP into a region of interest and uses electroporation to deliver the construct into cells. This unit describes in detail how single and small groups of cells (n<10) that express PAGFP can be made visually distinguishable from the host population using the photoactivation process. Included is a means to maximize the fluorescence increase due to photoactivated GFP signal and to reduce photobleaching. Briefly outlined are previously developed chick culture and time-lapse imaging techniques to allow for the subsequent monitoring of photoactivated cell migratory behaviors. The technique has the potential to be a less-invasive, accurate tool for in vivo studies that involve following cell lineage and cell migration." @default.
- W1501059600 created "2016-06-24" @default.
- W1501059600 creator A5002169187 @default.
- W1501059600 creator A5021609999 @default.
- W1501059600 date "2005-09-01" @default.
- W1501059600 modified "2023-10-16" @default.
- W1501059600 title "In Vivo Marking of Single Cells in Chick Embryos Using Photoactivation of GFP" @default.
- W1501059600 cites W1988326309 @default.
- W1501059600 cites W2032579724 @default.
- W1501059600 cites W2056768791 @default.
- W1501059600 cites W2069496952 @default.
- W1501059600 cites W2079760054 @default.
- W1501059600 cites W2088864569 @default.
- W1501059600 cites W2113231837 @default.
- W1501059600 cites W2140034535 @default.
- W1501059600 cites W2157321546 @default.
- W1501059600 cites W46841966 @default.
- W1501059600 cites W63274132 @default.
- W1501059600 doi "https://doi.org/10.1002/0471143030.cb1208s28" @default.
- W1501059600 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18228460" @default.
- W1501059600 hasPublicationYear "2005" @default.
- W1501059600 type Work @default.
- W1501059600 sameAs 1501059600 @default.
- W1501059600 citedByCount "7" @default.
- W1501059600 countsByYear W15010596002013 @default.
- W1501059600 countsByYear W15010596002015 @default.
- W1501059600 countsByYear W15010596002016 @default.
- W1501059600 countsByYear W15010596002017 @default.
- W1501059600 countsByYear W15010596002022 @default.
- W1501059600 crossrefType "journal-article" @default.
- W1501059600 hasAuthorship W1501059600A5002169187 @default.
- W1501059600 hasAuthorship W1501059600A5021609999 @default.
- W1501059600 hasConcept C104317684 @default.
- W1501059600 hasConcept C120665830 @default.
- W1501059600 hasConcept C121332964 @default.
- W1501059600 hasConcept C12554922 @default.
- W1501059600 hasConcept C142613039 @default.
- W1501059600 hasConcept C144024400 @default.
- W1501059600 hasConcept C1491633281 @default.
- W1501059600 hasConcept C149923435 @default.
- W1501059600 hasConcept C15526883 @default.
- W1501059600 hasConcept C186852380 @default.
- W1501059600 hasConcept C196843134 @default.
- W1501059600 hasConcept C207001950 @default.
- W1501059600 hasConcept C2908647359 @default.
- W1501059600 hasConcept C31332276 @default.
- W1501059600 hasConcept C54355233 @default.
- W1501059600 hasConcept C86803240 @default.
- W1501059600 hasConcept C91881484 @default.
- W1501059600 hasConcept C95444343 @default.
- W1501059600 hasConceptScore W1501059600C104317684 @default.
- W1501059600 hasConceptScore W1501059600C120665830 @default.
- W1501059600 hasConceptScore W1501059600C121332964 @default.
- W1501059600 hasConceptScore W1501059600C12554922 @default.
- W1501059600 hasConceptScore W1501059600C142613039 @default.
- W1501059600 hasConceptScore W1501059600C144024400 @default.
- W1501059600 hasConceptScore W1501059600C1491633281 @default.
- W1501059600 hasConceptScore W1501059600C149923435 @default.
- W1501059600 hasConceptScore W1501059600C15526883 @default.
- W1501059600 hasConceptScore W1501059600C186852380 @default.
- W1501059600 hasConceptScore W1501059600C196843134 @default.
- W1501059600 hasConceptScore W1501059600C207001950 @default.
- W1501059600 hasConceptScore W1501059600C2908647359 @default.
- W1501059600 hasConceptScore W1501059600C31332276 @default.
- W1501059600 hasConceptScore W1501059600C54355233 @default.
- W1501059600 hasConceptScore W1501059600C86803240 @default.
- W1501059600 hasConceptScore W1501059600C91881484 @default.
- W1501059600 hasConceptScore W1501059600C95444343 @default.
- W1501059600 hasIssue "1" @default.
- W1501059600 hasLocation W15010596001 @default.
- W1501059600 hasLocation W15010596002 @default.
- W1501059600 hasOpenAccess W1501059600 @default.
- W1501059600 hasPrimaryLocation W15010596001 @default.
- W1501059600 hasRelatedWork W1501059600 @default.
- W1501059600 hasRelatedWork W1978769331 @default.
- W1501059600 hasRelatedWork W2015089421 @default.
- W1501059600 hasRelatedWork W2045006037 @default.
- W1501059600 hasRelatedWork W2054411504 @default.
- W1501059600 hasRelatedWork W2198850752 @default.
- W1501059600 hasRelatedWork W2315602870 @default.
- W1501059600 hasRelatedWork W2908047276 @default.
- W1501059600 hasRelatedWork W4224837766 @default.
- W1501059600 hasRelatedWork W4253752195 @default.
- W1501059600 hasVolume "28" @default.
- W1501059600 isParatext "false" @default.
- W1501059600 isRetracted "false" @default.
- W1501059600 magId "1501059600" @default.
- W1501059600 workType "article" @default.