Matches in SemOpenAlex for { <https://semopenalex.org/work/W1501090807> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W1501090807 endingPage "5682" @default.
- W1501090807 startingPage "5678" @default.
- W1501090807 abstract "Ferrochelatase activity in human liver has been extensively characterized in the mitochondrial fraction by kinetic study of the enzyme in initial velocity conditions. We found that human liver mitochondrial membranes contain large amounts of endogenous metals that are substrates for the enzyme, leading to a lack of linearity of the activity as function of protein concentration. This lack of linearity is mainly due to a high zinc-chelatase activity with endogenous zinc. Under optimal experimental conditions, the maximum velocity for iron incorporation was 8.7 nmol of protoheme/h/mg of protein, and the maximum velocity for zinc incorporation was 4.3 nmol of zinc-protoporphyrin/h/mg of protein. The Michaelis constant for protoporphyrin IX was (i) dependent on the amount of protein when the overall chelatase reactions were measured but (ii) independent of the amount of protein when only zinc-chelatase activity was measured (Km = 0.5 microM). The Michaelis constants for iron and zinc were 0.35 and 0.08 microM, respectively, and the inhibitory constants for competitive incorporation of iron and zinc were KIFe/Zn = 0.12 microM and KIZn/Fe = 0.58 microM. The affinity of the enzyme for zinc lowers the actual determination of ferrochelatase activity with iron as substrate. Furthermore, when measuring ferrochelatase (e.g. in liver biopsy), endogenous zinc content in the biological sample must be taken into account." @default.
- W1501090807 created "2016-06-24" @default.
- W1501090807 creator A5022542179 @default.
- W1501090807 creator A5026204772 @default.
- W1501090807 creator A5047658239 @default.
- W1501090807 date "1984-05-01" @default.
- W1501090807 modified "2023-10-14" @default.
- W1501090807 title "Kinetic studies of human liver ferrochelatase. Role of endogenous metals." @default.
- W1501090807 cites W1490247781 @default.
- W1501090807 cites W1537985872 @default.
- W1501090807 cites W1541059747 @default.
- W1501090807 cites W1606741128 @default.
- W1501090807 cites W2008787274 @default.
- W1501090807 cites W2022780552 @default.
- W1501090807 cites W2048730285 @default.
- W1501090807 cites W2052342875 @default.
- W1501090807 cites W2068597663 @default.
- W1501090807 cites W2073129725 @default.
- W1501090807 cites W2075263381 @default.
- W1501090807 cites W2166660261 @default.
- W1501090807 cites W2225388783 @default.
- W1501090807 cites W2230022303 @default.
- W1501090807 cites W2398944594 @default.
- W1501090807 cites W2475930333 @default.
- W1501090807 cites W575512628 @default.
- W1501090807 doi "https://doi.org/10.1016/s0021-9258(18)91067-7" @default.
- W1501090807 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6425295" @default.
- W1501090807 hasPublicationYear "1984" @default.
- W1501090807 type Work @default.
- W1501090807 sameAs 1501090807 @default.
- W1501090807 citedByCount "46" @default.
- W1501090807 countsByYear W15010908072012 @default.
- W1501090807 countsByYear W15010908072017 @default.
- W1501090807 countsByYear W15010908072018 @default.
- W1501090807 countsByYear W15010908072021 @default.
- W1501090807 crossrefType "journal-article" @default.
- W1501090807 hasAuthorship W1501090807A5022542179 @default.
- W1501090807 hasAuthorship W1501090807A5026204772 @default.
- W1501090807 hasAuthorship W1501090807A5047658239 @default.
- W1501090807 hasBestOaLocation W15010908071 @default.
- W1501090807 hasConcept C121332964 @default.
- W1501090807 hasConcept C135889238 @default.
- W1501090807 hasConcept C16613235 @default.
- W1501090807 hasConcept C181199279 @default.
- W1501090807 hasConcept C185592680 @default.
- W1501090807 hasConcept C2776217839 @default.
- W1501090807 hasConcept C2776418720 @default.
- W1501090807 hasConcept C2781149050 @default.
- W1501090807 hasConcept C55493867 @default.
- W1501090807 hasConcept C62520636 @default.
- W1501090807 hasConceptScore W1501090807C121332964 @default.
- W1501090807 hasConceptScore W1501090807C135889238 @default.
- W1501090807 hasConceptScore W1501090807C16613235 @default.
- W1501090807 hasConceptScore W1501090807C181199279 @default.
- W1501090807 hasConceptScore W1501090807C185592680 @default.
- W1501090807 hasConceptScore W1501090807C2776217839 @default.
- W1501090807 hasConceptScore W1501090807C2776418720 @default.
- W1501090807 hasConceptScore W1501090807C2781149050 @default.
- W1501090807 hasConceptScore W1501090807C55493867 @default.
- W1501090807 hasConceptScore W1501090807C62520636 @default.
- W1501090807 hasIssue "9" @default.
- W1501090807 hasLocation W15010908071 @default.
- W1501090807 hasOpenAccess W1501090807 @default.
- W1501090807 hasPrimaryLocation W15010908071 @default.
- W1501090807 hasRelatedWork W1501090807 @default.
- W1501090807 hasRelatedWork W1984748335 @default.
- W1501090807 hasRelatedWork W1997565606 @default.
- W1501090807 hasRelatedWork W2059265220 @default.
- W1501090807 hasRelatedWork W2132012965 @default.
- W1501090807 hasRelatedWork W2951104772 @default.
- W1501090807 hasRelatedWork W3005003046 @default.
- W1501090807 hasRelatedWork W4244594731 @default.
- W1501090807 hasRelatedWork W2508177604 @default.
- W1501090807 hasRelatedWork W2521883233 @default.
- W1501090807 hasVolume "259" @default.
- W1501090807 isParatext "false" @default.
- W1501090807 isRetracted "false" @default.
- W1501090807 magId "1501090807" @default.
- W1501090807 workType "article" @default.