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- W1501298832 abstract "The globin mRNA sequences present in duck erythroblast nuclei appear under non-denaturing conditions to be associated with heterogeneous nuclear RNA (hnRNA) molecules of various sizes. Under denaturing conditions, however, the bulk of the globin mRNA sequences associated with hnRNA are released as molecules of size close to that of the active globin mRNA. To find out whether hydrogen-bonded structures occur in situ or arise after RNA extraction. nuclei were treated with aminomethyltrioxalen and exposed to ultraviolet light. This treatment generates covalent links between opposite strands of double-stranded nucleic acids, which were visualised by electron microscopy. It appears that, after cross-linking, a fraction of the globin mRNA sequences present in nuclei is associated with high-molecular-weight hnRNA molecules by a link which is resistant to stringent denaturing conditions. In particular globin mRNA sequences are found associated with a band of 0.9×106 molecular weight approximately. It is suggested that within the erythroblast nucleus, globin mRNA sequences are associated by hydrogen bonds with RNA of high molecular weight. These structures may represent intermediate steps in globin mRNA processing." @default.
- W1501298832 created "2016-06-24" @default.
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- W1501298832 date "1979-03-01" @default.
- W1501298832 modified "2023-10-05" @default.
- W1501298832 title "Globin mRNA Precursor. Cross-Linking in situ of Double-Stranded Segments with Aminomethyltrioxalen" @default.
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- W1501298832 doi "https://doi.org/10.1111/j.1432-1033.1979.tb12955.x" @default.
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