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- W1501371299 abstract "In the assessment of DNA sequences involved in the control of gene transcription, a common approach has been to transfect cell lines with genetic constructs in which the regulatory sequences under investigation are used to control the expression of a reporter gene. When such analyses involve regulatory sequences whose functions are thought to be cell type or tissue specific, it is often necessary to examine reporter expression in numerous cell lines, representing a variety of cell types, and to compare levels of expression between these cell lines. The technique described in this chapter permits an accurate determination of reporter gene expression by correcting for variations in transfection efficiency from sample to sample and for the inherent variations in transfection efficiency seen among various cell lines. As with any such data normalization, care needs to be taken to assure that all of the assumptions of such normalization do, in fact, hold true for any given experimental condition. Of primary concern is to determine empirically that the response measured is proportional to the amount of DNA used for transfection. In other words, a linear range must be established for each fluorescent protein construct in each cell type." @default.
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- W1501371299 date "1999-01-01" @default.
- W1501371299 modified "2023-10-17" @default.
- W1501371299 title "[17] Flow cytometric analysis of transcription: Use of green fluorescent protein variants to control transfection efficiency" @default.
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- W1501371299 doi "https://doi.org/10.1016/s0076-6879(99)02019-4" @default.
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