Matches in SemOpenAlex for { <https://semopenalex.org/work/W150147244> ?p ?o ?g. }
- W150147244 endingPage "C172" @default.
- W150147244 startingPage "C163" @default.
- W150147244 abstract "The role of cytosolic free Ca2+ concentration, [Cai], in mediating insulin's stimulatory effect on glucose transport was investigated in isolated Ca2+-tolerant rat ventricular cells. Approximately 98% of glucose transport in isolated myocytes was inhibited by phloretin. Insulin-accelerated glucose transport by 50-115% over basal transport rate. Removal of extracellular Ca2+ had no effect on either the basal transport rate or insulin's stimulatory action, indicating that extracellular Ca2+ was not necessary for insulin's effect to be manifest. Addition of A23187 had no effect on glucose transport rate. Under basal conditions, [Cai] was 167 +/- 12 nM as measured by fura-2 fluorescence and 239 +/- 22 nM by null-point titration with arsenazo III. Loading cells with fura-2 did not affect basal glucose transport rates. In addition, the stimulatory effect of insulin on glucose transport was preserved in fura-2 loaded cells. In paired experiments, insulin did not increase [Cai] as measured by fura-2 fluorescence or null-point titration despite acceleration of glucose transport. In contrast, addition of KCl (40 mM) increased [Cai] from 168 +/- 30 to 287 +/- 51 nM and resulted in 50% reduction in glucose transport rate. In other experiments designed to control for the hyperosmolar effects of KCl, NaCl (40 mM) caused no change in [Cai] but also inhibited glucose transport rate by 50%. We conclude that an elevation in [Cai] is unlikely to be the intracellular signal mediating insulin's effect on glucose transport since insulin's stimulatory effect was not reduced by Ca2+ -free media, insulin had no detectable effect on [Cai], and elevation of [Cai] by KCl did not result in stimulation of glucose transport." @default.
- W150147244 created "2016-06-24" @default.
- W150147244 creator A5028158014 @default.
- W150147244 creator A5085167135 @default.
- W150147244 creator A5089781525 @default.
- W150147244 date "1987-02-01" @default.
- W150147244 modified "2023-10-05" @default.
- W150147244 title "Cytosolic free calcium concentration and glucose transport in isolated cardiac myocytes" @default.
- W150147244 cites W1236544414 @default.
- W150147244 cites W1530516343 @default.
- W150147244 cites W1669747360 @default.
- W150147244 cites W1775749144 @default.
- W150147244 cites W1832574527 @default.
- W150147244 cites W1980107664 @default.
- W150147244 cites W1994476430 @default.
- W150147244 cites W1997653422 @default.
- W150147244 cites W2001736800 @default.
- W150147244 cites W2002096718 @default.
- W150147244 cites W2018015055 @default.
- W150147244 cites W2030278074 @default.
- W150147244 cites W2043618459 @default.
- W150147244 cites W2055784873 @default.
- W150147244 cites W2060295893 @default.
- W150147244 cites W2060755886 @default.
- W150147244 cites W2067119870 @default.
- W150147244 cites W2072360524 @default.
- W150147244 cites W2083494154 @default.
- W150147244 cites W2087090971 @default.
- W150147244 cites W2096104825 @default.
- W150147244 cites W2113943014 @default.
- W150147244 cites W2122423123 @default.
- W150147244 cites W2141260882 @default.
- W150147244 cites W2146927479 @default.
- W150147244 cites W22143531 @default.
- W150147244 cites W303088254 @default.
- W150147244 cites W4251494161 @default.
- W150147244 doi "https://doi.org/10.1152/ajpcell.1987.252.2.c163" @default.
- W150147244 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3103463" @default.
- W150147244 hasPublicationYear "1987" @default.
- W150147244 type Work @default.
- W150147244 sameAs 150147244 @default.
- W150147244 citedByCount "99" @default.
- W150147244 countsByYear W1501472442012 @default.
- W150147244 countsByYear W1501472442013 @default.
- W150147244 countsByYear W1501472442015 @default.
- W150147244 countsByYear W1501472442017 @default.
- W150147244 countsByYear W1501472442018 @default.
- W150147244 countsByYear W1501472442021 @default.
- W150147244 countsByYear W1501472442022 @default.
- W150147244 crossrefType "journal-article" @default.
- W150147244 hasAuthorship W150147244A5028158014 @default.
- W150147244 hasAuthorship W150147244A5085167135 @default.
- W150147244 hasAuthorship W150147244A5089781525 @default.
- W150147244 hasConcept C126322002 @default.
- W150147244 hasConcept C134018914 @default.
- W150147244 hasConcept C161573976 @default.
- W150147244 hasConcept C185592680 @default.
- W150147244 hasConcept C2777866211 @default.
- W150147244 hasConcept C2778024521 @default.
- W150147244 hasConcept C2778423325 @default.
- W150147244 hasConcept C2779306644 @default.
- W150147244 hasConcept C28406088 @default.
- W150147244 hasConcept C519063684 @default.
- W150147244 hasConcept C55493867 @default.
- W150147244 hasConcept C71924100 @default.
- W150147244 hasConcept C79879829 @default.
- W150147244 hasConcept C86803240 @default.
- W150147244 hasConceptScore W150147244C126322002 @default.
- W150147244 hasConceptScore W150147244C134018914 @default.
- W150147244 hasConceptScore W150147244C161573976 @default.
- W150147244 hasConceptScore W150147244C185592680 @default.
- W150147244 hasConceptScore W150147244C2777866211 @default.
- W150147244 hasConceptScore W150147244C2778024521 @default.
- W150147244 hasConceptScore W150147244C2778423325 @default.
- W150147244 hasConceptScore W150147244C2779306644 @default.
- W150147244 hasConceptScore W150147244C28406088 @default.
- W150147244 hasConceptScore W150147244C519063684 @default.
- W150147244 hasConceptScore W150147244C55493867 @default.
- W150147244 hasConceptScore W150147244C71924100 @default.
- W150147244 hasConceptScore W150147244C79879829 @default.
- W150147244 hasConceptScore W150147244C86803240 @default.
- W150147244 hasIssue "2" @default.
- W150147244 hasLocation W1501472441 @default.
- W150147244 hasLocation W1501472442 @default.
- W150147244 hasOpenAccess W150147244 @default.
- W150147244 hasPrimaryLocation W1501472441 @default.
- W150147244 hasRelatedWork W1966225706 @default.
- W150147244 hasRelatedWork W1979204365 @default.
- W150147244 hasRelatedWork W1979754845 @default.
- W150147244 hasRelatedWork W2006513370 @default.
- W150147244 hasRelatedWork W2037205718 @default.
- W150147244 hasRelatedWork W2087369668 @default.
- W150147244 hasRelatedWork W2103726562 @default.
- W150147244 hasRelatedWork W2119302231 @default.
- W150147244 hasRelatedWork W2122338601 @default.
- W150147244 hasRelatedWork W2223043162 @default.
- W150147244 hasVolume "252" @default.
- W150147244 isParatext "false" @default.