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- W1501708095 abstract "This chapter details methods for the isolation of microsomes that have been successfully used for several species and the procedure for the purification of CYP6D1 from house fly microsomes. The P450 monooxygenases of insects have several functional roles, including growth, development, resistance to pesticides, and tolerance to plant toxins. Monooxygenases are intimately involved in fatty acid metabolism and in the biosynthesis of insect hormones and pheromones including 20-hydroxyecdysone and juvenile hormone. The study of insect P450s has been hampered by the small size of the organisms and the relative instability of microsomal preparations. The general methods used to isolate microsomes from several insect species include the large-scale and small-scale methods. Hydrophobic chromatography would be the optimal first step if cytochrome P450s have similar hydrophobic natures that are different from other proteins." @default.
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- W1501708095 date "1996-01-01" @default.
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- W1501708095 title "[32] Preparation of microsomes from insects and purification of CYP6D1 from house flies" @default.
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- W1501708095 doi "https://doi.org/10.1016/s0076-6879(96)72034-7" @default.
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