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- W1501777709 abstract "In the implementation of CARS nanoscopy, signal strength decreases with focal volume size decreasing. A crucial problem that remains to be solved is whether the reduced signal generated in the suppressed focal volume can be detected.Here reported is a theoretical analysis of detection limit(DL) to time-resolved CARS(T-CARS) nanoscopy based on our proposed additional probe-beam-induced phonon depletion(APIPD) method for the low concentration samples. In order to acquire a detailed shot-noise limited signal-to-noise(SNR) and the involved parameters to evaluate DL, the T-CARS process is described with full quantum theory to estimate the extreme power density levels of the pump and Stokes beams determined by saturation behavior of coherent phonons, which are both actually on the order of ～ 109W/cm2. When the pump and Stokes intensities reach such values and the total intensity of the excitation beams arrives at a maximum tolerable by most biological samples in a certain suppressed focal volume(40-nm suppressed focal scale in APIPD method), the DL correspondingly varies with exposure time, for example, DL values are 103 and 102when exposure times are 20 ms and200 ms respectively." @default.
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- W1501777709 date "2014-01-01" @default.
- W1501777709 modified "2023-10-18" @default.
- W1501777709 title "Analysis of detection limit to time-resolved coherent anti-Stokes Raman scattering nanoscopy" @default.
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