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• W1502155306 abstract "Based on the results of mapping of ggt, eight strains were selected from a gene library of E. coli. One of the strains harboring pLC9-12 was found to show 14 times higher gamma-glutamyltranspeptidase activity per cell than the wild type strain. The ggt was subcloned to the BamHI site of pUC18 and the recombinant plasmid pSH101 was obtained. Ggt- phenotype of gamma-glutamyltranspeptidase-deficient mutants was complemented by pSH101. The specific activity of the enzyme in cells harboring pSH101 was 37-fold higher than that in the wild type cells. gamma-Glutamyltranspeptidase was isolated from the periplasmic fraction of the cells by simple two steps and crystallized." @default.
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• W1502155306 date "1988-01-01" @default.
• W1502155306 modified "2023-10-03" @default.
• W1502155306 title "Molecular cloning of K-12 and rapid isolation of γ-glutamyltranspeptidase" @default.
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• W1502155306 doi "https://doi.org/10.1016/0006-291x(88)90482-2" @default.
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