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• W1502292292 abstract "Objective: The purpose of this study was to evaluate the quantitative expressions of BAG1, BAX and BCL-2 in human embryos with different fragmentation grades as derived from assisted reproduction technology (ART). Materials and Methods: Fragmented and normal human 8-cell embryos were scored according to the degree of fragmentation with an inverted microscope and divided into four grades (grade І: no or minimal fragmentation ( 25% fragmentation and grade ІV: apoptotic induced embryos with actinomycin D). In this study, TUNEL labeling was initially used to detect apoptosis, and then revers transcription polymerase chain reaction (RT-PCR) and quantitative PCR were used to define the quantitative expressions of experimental genes in human embryos with different fragmentation grades. Results: The results of TUNEL labeling showed that embryos with higher fragmentation had a high number of apoptotic bodies. The results of RT-PCR and q-PCR analyses showed a significantly decreased amount of BAGI transcript expression from group I to group IV. The highest expression of BAX gene was observed in group II, however, the transcript of BCL-2 gene was not observed in any of the experimental groups. The effect of actinomycin D on transcript expression amounts of experimental genes in apoptotic induced embryos (group IV) compared to control embryos (group I) showed a significant" @default.
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• W1502292292 date "2010-01-01" @default.
• W1502292292 modified "2023-09-27" @default.
• W1502292292 title "QUANTITATIVE EXPRESSION OF BAG1, BAX AND BCL-2 GENES IN HUMAN EMBRYOS WITH DIFFERENT FRAGMENTATION GRADES DERIVED FROM ART" @default.
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