FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1502446689> ?p ?o ?g. }

• W1502446689 endingPage "6162" @default.
• W1502446689 startingPage "6156" @default.
• W1502446689 abstract "Two cytosolic proteins of murine epidermis or porcine spleen with molecular masses of 37 kDa (p37) and 50 kDa (p50) are differentially phosphorylated in vitro by the purified protein kinase C (PKC) isoenzymes α, β, γ (cPKC) and PKCδ. p37, identified as annexin I, is preferentially phosphorylated by cPKC, whereas p50, identified as elongation factor eEF-1α, is phosphorylated with much greater efficacy by PKCδ than by cPKC. Using the recombinant PKC isoenzymes α, β, γ, δ, ε, η, and ϛ, we could show that purified eEF-1α is indeed a specific substrate of PKCδ. It is not significantly phosphorylated by PKCε, -η, and -ϛ and only slightly by PKCα, -β, and -γ. PKCδ phosphorylates eEF-1α at Thr-431 (based on the murine amino acid sequence). The peptide RFAVRDMRQTVAVGVIKAVDKK with a sequence corresponding to that of 422-443 from murine eEF-1α and containing Thr-431 is an absolutely specific substrate for the δ-type of PKC. The single basic amino acid close to Thr-431 (Arg-429) is essential for recognition of the peptide as a substrate by PKCδ and for the selectivity of this recognition. Substitution of Arg-429 by alanine abolishes the ability of PKCδ to phosphorylate the peptide, and insertion of additional basic amino acids in the vicinity of Thr-431 causes a complete loss of selectivity. Two cytosolic proteins of murine epidermis or porcine spleen with molecular masses of 37 kDa (p37) and 50 kDa (p50) are differentially phosphorylated in vitro by the purified protein kinase C (PKC) isoenzymes α, β, γ (cPKC) and PKCδ. p37, identified as annexin I, is preferentially phosphorylated by cPKC, whereas p50, identified as elongation factor eEF-1α, is phosphorylated with much greater efficacy by PKCδ than by cPKC. Using the recombinant PKC isoenzymes α, β, γ, δ, ε, η, and ϛ, we could show that purified eEF-1α is indeed a specific substrate of PKCδ. It is not significantly phosphorylated by PKCε, -η, and -ϛ and only slightly by PKCα, -β, and -γ. PKCδ phosphorylates eEF-1α at Thr-431 (based on the murine amino acid sequence). The peptide RFAVRDMRQTVAVGVIKAVDKK with a sequence corresponding to that of 422-443 from murine eEF-1α and containing Thr-431 is an absolutely specific substrate for the δ-type of PKC. The single basic amino acid close to Thr-431 (Arg-429) is essential for recognition of the peptide as a substrate by PKCδ and for the selectivity of this recognition. Substitution of Arg-429 by alanine abolishes the ability of PKCδ to phosphorylate the peptide, and insertion of additional basic amino acids in the vicinity of Thr-431 causes a complete loss of selectivity." @default.
• W1502446689 created "2016-06-24" @default.
• W1502446689 creator A5007487924 @default.
• W1502446689 creator A5041265058 @default.
• W1502446689 creator A5066192161 @default.
• W1502446689 creator A5076135264 @default.
• W1502446689 creator A5087936561 @default.
• W1502446689 date "1995-03-01" @default.
• W1502446689 modified "2023-10-17" @default.
• W1502446689 title "Protein Kinase Cσ-specific Phosphorylation of the Elongation Factor eEF-1α and an eEF-1α Peptide at Threonine 431" @default.
• W1502446689 cites W1485657773 @default.
• W1502446689 cites W1487365829 @default.
• W1502446689 cites W1506717390 @default.
• W1502446689 cites W1508860572 @default.
• W1502446689 cites W1509211541 @default.
• W1502446689 cites W1518539185 @default.
• W1502446689 cites W1528279723 @default.
• W1502446689 cites W1550017207 @default.
• W1502446689 cites W1553713007 @default.
• W1502446689 cites W1554882994 @default.
• W1502446689 cites W1557051865 @default.
• W1502446689 cites W1557709748 @default.
• W1502446689 cites W1564302359 @default.
• W1502446689 cites W1568928604 @default.
• W1502446689 cites W1569096762 @default.
• W1502446689 cites W1583681990 @default.
• W1502446689 cites W1597634219 @default.
• W1502446689 cites W1650137855 @default.
• W1502446689 cites W1706568612 @default.
• W1502446689 cites W1758789982 @default.
• W1502446689 cites W1850214287 @default.
• W1502446689 cites W1965098419 @default.
• W1502446689 cites W1966601917 @default.
• W1502446689 cites W1984593949 @default.
• W1502446689 cites W1985695864 @default.
• W1502446689 cites W1991318616 @default.
• W1502446689 cites W1991972636 @default.
• W1502446689 cites W1993282564 @default.
• W1502446689 cites W1995499596 @default.
• W1502446689 cites W2001668713 @default.
• W1502446689 cites W2002468039 @default.
• W1502446689 cites W2005304515 @default.
• W1502446689 cites W2007319015 @default.
• W1502446689 cites W2009856702 @default.
• W1502446689 cites W2015268031 @default.
• W1502446689 cites W2017304947 @default.
• W1502446689 cites W2027355347 @default.
• W1502446689 cites W2027673151 @default.
• W1502446689 cites W2035985010 @default.
• W1502446689 cites W2041131340 @default.
• W1502446689 cites W2043610860 @default.
• W1502446689 cites W2045284446 @default.
• W1502446689 cites W2045957363 @default.
• W1502446689 cites W2054800842 @default.
• W1502446689 cites W2059278612 @default.
• W1502446689 cites W2060622769 @default.
• W1502446689 cites W2065135980 @default.
• W1502446689 cites W2073159198 @default.
• W1502446689 cites W2073694135 @default.
• W1502446689 cites W2075249267 @default.
• W1502446689 cites W2079982068 @default.
• W1502446689 cites W2080853378 @default.
• W1502446689 cites W2080933052 @default.
• W1502446689 cites W2086524338 @default.
• W1502446689 cites W2088072731 @default.
• W1502446689 cites W2096077209 @default.
• W1502446689 cites W2103719933 @default.
• W1502446689 cites W2136674751 @default.
• W1502446689 cites W2426793753 @default.
• W1502446689 cites W3127324270 @default.
• W1502446689 cites W48984577 @default.
• W1502446689 doi "https://doi.org/10.1074/jbc.270.11.6156" @default.
• W1502446689 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7890750" @default.
• W1502446689 hasPublicationYear "1995" @default.
• W1502446689 type Work @default.
• W1502446689 sameAs 1502446689 @default.
• W1502446689 citedByCount "75" @default.
• W1502446689 countsByYear W15024466892012 @default.
• W1502446689 countsByYear W15024466892013 @default.
• W1502446689 countsByYear W15024466892014 @default.
• W1502446689 countsByYear W15024466892015 @default.
• W1502446689 countsByYear W15024466892016 @default.
• W1502446689 countsByYear W15024466892018 @default.
• W1502446689 countsByYear W15024466892020 @default.
• W1502446689 countsByYear W15024466892021 @default.
• W1502446689 crossrefType "journal-article" @default.
• W1502446689 hasAuthorship W1502446689A5007487924 @default.
• W1502446689 hasAuthorship W1502446689A5041265058 @default.
• W1502446689 hasAuthorship W1502446689A5066192161 @default.
• W1502446689 hasAuthorship W1502446689A5076135264 @default.
• W1502446689 hasAuthorship W1502446689A5087936561 @default.
• W1502446689 hasBestOaLocation W15024466891 @default.
• W1502446689 hasConcept C104317684 @default.
• W1502446689 hasConcept C11960822 @default.
• W1502446689 hasConcept C119795356 @default.
• W1502446689 hasConcept C153911025 @default.
• W1502446689 hasConcept C167625842 @default.
• W1502446689 hasConcept C181199279 @default.

• next