FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1502544172> ?p ?o ?g. }

• W1502544172 endingPage "20641" @default.
• W1502544172 startingPage "20636" @default.
• W1502544172 abstract "N-Ethylmaleimide-sensitive factor (NSF) was originally characterized as the protein that restores in vitro protein transport activity of the Golgi membranes inactivated by N-ethylmaleimide. This protein has two homologous regions, each containing the consensus sequence for nucleotide binding, and possesses ATPase activity. To investigate the role of the two nucleotide-binding regions in NSF, we have replaced two lysyl residues (Lys-274 and Lys-557) located in the consensus sequences with glutamine or methionine via site-directed mutagenesis. The mutant NSF proteins in which Lys-274 was replaced had no ability to restore protein transport between N-ethylmaleimide-treated Golgi membranes and, in addition, inhibited the protein transport assay using normal Golgi membranes. This inhibition, which was eliminated by N-ethylmaleimide treatment, was caused by the impairment of the function of donor Golgi membranes. Although wild-type NSF showed a protective effect against inhibition by the Lys-274 mutant NSF protein when added at the start of the protein transport assay, its protective effect diminished after the time for the formation of transport vesicles had passed. These results support the idea that NSF incorporated into transport vesicles is nonexchangeable for exogenously added NSF. On the other hand, the mutant proteins in which Lys-557 was replaced had slight but significant protein transport activity. They did not inhibit the protein transport assay using normal Golgi membranes. The mutant NSF proteins in which Lys-274 and Lys-557 were replaced had about 20 and 25% of the ATPase activities of wild-type NSF, respectively. Their ATPase activities were sensitive to N-ethylmaleimide and dependent on their protein concentrations, as observed in wild-type NSF." @default.
• W1502544172 created "2016-06-24" @default.
• W1502544172 creator A5027802926 @default.
• W1502544172 creator A5027831160 @default.
• W1502544172 creator A5082076285 @default.
• W1502544172 date "1994-08-01" @default.
• W1502544172 modified "2023-09-30" @default.
• W1502544172 title "Role of two nucleotide-binding regions in an N-ethylmaleimide-sensitive factor involved in vesicle-mediated protein transport." @default.
• W1502544172 cites W1572521445 @default.
• W1502544172 cites W1592007354 @default.
• W1502544172 cites W1971016259 @default.
• W1502544172 cites W1972189437 @default.
• W1502544172 cites W1984634392 @default.
• W1502544172 cites W1986828137 @default.
• W1502544172 cites W1997168691 @default.
• W1502544172 cites W1997278714 @default.
• W1502544172 cites W1998020191 @default.
• W1502544172 cites W2030822379 @default.
• W1502544172 cites W2036277985 @default.
• W1502544172 cites W2036931150 @default.
• W1502544172 cites W2039115958 @default.
• W1502544172 cites W2048001750 @default.
• W1502544172 cites W2056541157 @default.
• W1502544172 cites W2065220292 @default.
• W1502544172 cites W2078691152 @default.
• W1502544172 cites W2085680244 @default.
• W1502544172 cites W2099047634 @default.
• W1502544172 cites W2100837269 @default.
• W1502544172 cites W2106141363 @default.
• W1502544172 cites W2138270253 @default.
• W1502544172 doi "https://doi.org/10.1016/s0021-9258(17)32040-9" @default.
• W1502544172 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8051162" @default.
• W1502544172 hasPublicationYear "1994" @default.
• W1502544172 type Work @default.
• W1502544172 sameAs 1502544172 @default.
• W1502544172 citedByCount "48" @default.
• W1502544172 countsByYear W15025441722012 @default.
• W1502544172 countsByYear W15025441722013 @default.
• W1502544172 countsByYear W15025441722015 @default.
• W1502544172 crossrefType "journal-article" @default.
• W1502544172 hasAuthorship W1502544172A5027802926 @default.
• W1502544172 hasAuthorship W1502544172A5027831160 @default.
• W1502544172 hasAuthorship W1502544172A5082076285 @default.
• W1502544172 hasBestOaLocation W15025441721 @default.
• W1502544172 hasConcept C104317684 @default.
• W1502544172 hasConcept C12554922 @default.
• W1502544172 hasConcept C130316041 @default.
• W1502544172 hasConcept C185592680 @default.
• W1502544172 hasConcept C2777646807 @default.
• W1502544172 hasConcept C41625074 @default.
• W1502544172 hasConcept C512185932 @default.
• W1502544172 hasConcept C55493867 @default.
• W1502544172 hasConcept C86803240 @default.
• W1502544172 hasConcept C95444343 @default.
• W1502544172 hasConceptScore W1502544172C104317684 @default.
• W1502544172 hasConceptScore W1502544172C12554922 @default.
• W1502544172 hasConceptScore W1502544172C130316041 @default.
• W1502544172 hasConceptScore W1502544172C185592680 @default.
• W1502544172 hasConceptScore W1502544172C2777646807 @default.
• W1502544172 hasConceptScore W1502544172C41625074 @default.
• W1502544172 hasConceptScore W1502544172C512185932 @default.
• W1502544172 hasConceptScore W1502544172C55493867 @default.
• W1502544172 hasConceptScore W1502544172C86803240 @default.
• W1502544172 hasConceptScore W1502544172C95444343 @default.
• W1502544172 hasIssue "32" @default.
• W1502544172 hasLocation W15025441721 @default.
• W1502544172 hasOpenAccess W1502544172 @default.
• W1502544172 hasPrimaryLocation W15025441721 @default.
• W1502544172 hasRelatedWork W1015203990 @default.
• W1502544172 hasRelatedWork W1187715755 @default.
• W1502544172 hasRelatedWork W1502544172 @default.
• W1502544172 hasRelatedWork W1512030089 @default.
• W1502544172 hasRelatedWork W1598194566 @default.
• W1502544172 hasRelatedWork W2018943552 @default.
• W1502544172 hasRelatedWork W2038699852 @default.
• W1502544172 hasRelatedWork W2168869282 @default.
• W1502544172 hasRelatedWork W2912747440 @default.
• W1502544172 hasRelatedWork W4256419381 @default.
• W1502544172 hasVolume "269" @default.
• W1502544172 isParatext "false" @default.
• W1502544172 isRetracted "false" @default.
• W1502544172 magId "1502544172" @default.
• W1502544172 workType "article" @default.