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- W1502700970 abstract "Upon infection of Escherichia coli by phage T2, rapid disintegration of the bacterial nucleus is followed at 4 minutes by the formation of marginal vacuoles; after an additional 4 minutes a visible pool of phage-precursor DNA begins to develop. Even so early a step in this process as breakdown of the bacterial nucleus is inhibitable by chloramphenicol. Some 10 minutes after infection, phage DNA begins to condense into dense particles about the size and shape of phage, but lacking a protein membrane and hence extremely fragile, not surviving lysis in any recognizable form. The condensed particles then develop into an immature phage form, surrounded by an incomplete protein membrane which ruptures upon lysis, producing empty heads or doughnuts. The growth of the morphological DNA pool is unaffected by chloramphenicol when this is added 8 minutes or more after infection. No condensation of DNA occurs, however. Hence it is postulated that the condensation of DNA is produced by a yet unknown “condensation principle” of protein nature." @default.
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- W1502700970 date "1959-08-01" @default.
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- W1502700970 title "Electron microscopical studies of phage multiplication" @default.
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- W1502700970 doi "https://doi.org/10.1016/0042-6822(59)90050-9" @default.
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