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• W1502707983 abstract "Thymidine kinase (TK) and deoxycytidine kinase (dCK) activity levels, [3H]thymidine (TdR) and 5-bromo-2′-deoxyuridine (BUdR) incorporation and 5-fluoro-2′-deoxyuridine (FUdR) sensitivity have been compared in TK-deficient (TU63 and TU84) and normal (TU291 and M3b) strains of the myxomycete, Physarum polycephalum. The mutants had about 2% of the TK and 100% of the dCK activity of wild-type (wt) strains. They incorporated some TdR into both nuclear (nDNA) and mitochondrial DNA (mtDNA) but incorporated too little BUdR to give a buoyant density shift in nuclear DNA. They grew in the presence of levels of FUdR which completely blocked DNA synthesis in TU291. The FUdR sensitivity of strain M3b could be increased by supplementing growth medium with folic acid." @default.
• W1502707983 created "2016-06-24" @default.
• W1502707983 creator A5034516863 @default.
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• W1502707983 date "1980-04-01" @default.
• W1502707983 modified "2023-09-25" @default.
• W1502707983 title "Thymidine kinase-deficient mutants of Physarum polycephalum" @default.
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• W1502707983 doi "https://doi.org/10.1016/0014-4827(80)90274-8" @default.
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