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- W1502717177 abstract "After several hundred generations in vitro, HT cells originally derived from type 12 adenovirus (Ad.12)-induced hamster tumors have retained their malignant character, the capacity to synthesize Ad.12-specific antigen(s), and a significantly reduced capacity, compared with that of normal hamster cells, to produce infectious type 2 adenovirus (Ad.2) in response to superinfection. Of four independently derived cell lines studied, one (HT2) consistently produces less Ad.2 than the other three lines. No evidence has been obtained for “rescue” of Ad.12 among the yields from Ad.2-superinfected HT cells. The low yield from such cells is not due to poor adsorption of inoculum virus. Thirteen clones of two cell lines (HT2 and HT8) have been established after microdrop isolation of single cells. The fact that all clones tested have retained the three characteristics suggests genetic homogeneity of the parental populations. In particular, the differing extent to which synthesis of Ad.2 is depressed in the two parental lines is reflected in the response of their derived clones. The evidence argues against the idea that HT cell populations consist of a majority of genetically stable “resistant” and a minority of stably “susceptible” cells." @default.
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- W1502717177 date "1966-04-01" @default.
- W1502717177 modified "2023-09-25" @default.
- W1502717177 title "Properties of cells derived from adenovirus-induced hamster tumors by long-term in vitro cultivation" @default.
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- W1502717177 doi "https://doi.org/10.1016/0042-6822(66)90248-0" @default.
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