FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1502980550> ?p ?o ?g. }

• W1502980550 endingPage "342" @default.
• W1502980550 startingPage "332" @default.
• W1502980550 abstract "The nature of the response of conventional and CD5 + B cells to stimulation in vitro with optimal mitogenic concentrations of LPS was examined to elucidate the contributions of these B cell subsets in polyclonal B lymphocyte responses. Stimulation of murine splenic lymphocytes with LPS resulted in an increase in total biomass, peaking at 72 h of culture. The viability of the cultures remained high (>90%) until 48 h of culture. A combination of trypan blue and 7‐aminoactinomycin D (7AAD) exclusion in conjunction with PE‐anti‐CD5 and FITC‐anti‐B220 enabled more detailed analysis of the cultures. The total number of conventional B cells, viable and non‐viable, increased until 48 h of culture and then decreased when stimulated with LPS, while CD5 + B cells increased over the culture period. The numbers of conventional B cells in the control cultures decreased, but the CD5 + B cell numbers remained stable. An examination of the modes of death of the B cell subsets using 7AAD showed that unstimulated conventional B cells were apoptotic rather than degenerate but, following stimulation with LPS, apoptotic and degenerate cells were found. Apoptotic and degenerate CD5 + B cells were found in both stimulated and unstimulated cultures, but the percentage of these apoptotic and degenerate cells was increased significantly only at 72 h and 96 h of culture in stimulated cultures compared with 24 h onwards in the control cultures. Morphological analysis and gel electrophoretic studies of extracted DNA reflected these findings. It was also found that the increase in the number and percentage of non‐viable cells in the cultures was not equal to the decrease in the number and percentage of viable cells. Activation of B cells was examined using expression of B7‐1 (CD80) as a marker. When stimulated with LPS a greater proportion of conventional B cells expressed B7‐1 after 24 h of culture than in the control cultures; however, only at 72 h and 96 h of culture was the proportion of CD5 + B cells expressing B7‐1 significantly higher than in the control cultures. These results show that conventional B cells are stimulated to proliferate and to become activated by LPS and that death is apoptotic rather than degenerate or necrotic. CD5 + B cells were also shown to be stimulated by LPS; they became activated and death was delayed. The data suggest that in addition to the proliferative role, LPS acts to delay death and to activate conventional and CD5 + B cells." @default.
• W1502980550 created "2016-06-24" @default.
• W1502980550 creator A5002074299 @default.
• W1502980550 creator A5026815496 @default.
• W1502980550 creator A5064738633 @default.
• W1502980550 date "1998-07-01" @default.
• W1502980550 modified "2023-09-30" @default.
• W1502980550 title "Characterization of the mitogenic response of murine CD5⁺and conventional B lymphocytes to lipopolysaccharide" @default.
• W1502980550 cites W1588448038 @default.
• W1502980550 cites W1819822942 @default.
• W1502980550 cites W1903605698 @default.
• W1502980550 cites W1964130131 @default.
• W1502980550 cites W1966844155 @default.
• W1502980550 cites W1997452013 @default.
• W1502980550 cites W1998189911 @default.
• W1502980550 cites W2019450981 @default.
• W1502980550 cites W2041883879 @default.
• W1502980550 cites W2071147619 @default.
• W1502980550 cites W2073309247 @default.
• W1502980550 cites W2091673136 @default.
• W1502980550 cites W2109401564 @default.
• W1502980550 cites W2114918609 @default.
• W1502980550 cites W2116690382 @default.
• W1502980550 cites W2121794828 @default.
• W1502980550 cites W2124209307 @default.
• W1502980550 cites W2128587758 @default.
• W1502980550 cites W2140483100 @default.
• W1502980550 cites W2144225361 @default.
• W1502980550 doi "https://doi.org/10.1046/j.1440-1711.1998.00752.x" @default.
• W1502980550 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9723774" @default.
• W1502980550 hasPublicationYear "1998" @default.
• W1502980550 type Work @default.
• W1502980550 sameAs 1502980550 @default.
• W1502980550 citedByCount "2" @default.
• W1502980550 crossrefType "journal-article" @default.
• W1502980550 hasAuthorship W1502980550A5002074299 @default.
• W1502980550 hasAuthorship W1502980550A5026815496 @default.
• W1502980550 hasAuthorship W1502980550A5064738633 @default.
• W1502980550 hasConcept C113067387 @default.
• W1502980550 hasConcept C134018914 @default.
• W1502980550 hasConcept C153911025 @default.
• W1502980550 hasConcept C159654299 @default.
• W1502980550 hasConcept C190283241 @default.
• W1502980550 hasConcept C203014093 @default.
• W1502980550 hasConcept C24998067 @default.
• W1502980550 hasConcept C2778453870 @default.
• W1502980550 hasConcept C2778754761 @default.
• W1502980550 hasConcept C2780070996 @default.
• W1502980550 hasConcept C54355233 @default.
• W1502980550 hasConcept C553184892 @default.
• W1502980550 hasConcept C55493867 @default.
• W1502980550 hasConcept C81885089 @default.
• W1502980550 hasConcept C86803240 @default.
• W1502980550 hasConcept C956191 @default.
• W1502980550 hasConceptScore W1502980550C113067387 @default.
• W1502980550 hasConceptScore W1502980550C134018914 @default.
• W1502980550 hasConceptScore W1502980550C153911025 @default.
• W1502980550 hasConceptScore W1502980550C159654299 @default.
• W1502980550 hasConceptScore W1502980550C190283241 @default.
• W1502980550 hasConceptScore W1502980550C203014093 @default.
• W1502980550 hasConceptScore W1502980550C24998067 @default.
• W1502980550 hasConceptScore W1502980550C2778453870 @default.
• W1502980550 hasConceptScore W1502980550C2778754761 @default.
• W1502980550 hasConceptScore W1502980550C2780070996 @default.
• W1502980550 hasConceptScore W1502980550C54355233 @default.
• W1502980550 hasConceptScore W1502980550C553184892 @default.
• W1502980550 hasConceptScore W1502980550C55493867 @default.
• W1502980550 hasConceptScore W1502980550C81885089 @default.
• W1502980550 hasConceptScore W1502980550C86803240 @default.
• W1502980550 hasConceptScore W1502980550C956191 @default.
• W1502980550 hasFunder F4320322896 @default.
• W1502980550 hasIssue "4" @default.
• W1502980550 hasLocation W15029805501 @default.
• W1502980550 hasLocation W15029805502 @default.
• W1502980550 hasOpenAccess W1502980550 @default.
• W1502980550 hasPrimaryLocation W15029805501 @default.
• W1502980550 hasRelatedWork W1511425935 @default.
• W1502980550 hasRelatedWork W2065751381 @default.
• W1502980550 hasRelatedWork W2354104009 @default.
• W1502980550 hasRelatedWork W2364890193 @default.
• W1502980550 hasRelatedWork W2374430045 @default.
• W1502980550 hasRelatedWork W2379716325 @default.
• W1502980550 hasRelatedWork W2385730182 @default.
• W1502980550 hasRelatedWork W2386214274 @default.
• W1502980550 hasRelatedWork W2394354805 @default.
• W1502980550 hasRelatedWork W2790366641 @default.
• W1502980550 hasVolume "76" @default.
• W1502980550 isParatext "false" @default.
• W1502980550 isRetracted "false" @default.
• W1502980550 magId "1502980550" @default.
• W1502980550 workType "article" @default.