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- W1503144696 abstract "The termini of the kanamycin resistance transposon Tn5 consist of a pair of nearly identical insertion sequences named IS50L and IS50R arranged in inverted orientation. The ends of these IS50 elements consist of an extremely short (8 of 9 base-pairs) inverted repeat. Assays of IS50-mediated gene transposition from pBR322:: Tn5 and related bacterial plasmids to bacteriophage λ show that the two ends of IS50 are not equivalent as sites for transposase recognition. Pairs of outside (O) ends of IS50 are found to be used up to three orders of magnitude more efficiently than pairs of inside (I) ends, but only slightly more efficiently than one O end and one I end. These data suggest that the eight base-pair inverted repeats at the termini of IS50 comprise only a partial transposase recognition site, and that sequences at the O end beyond the common eight base-pairs are needed for efficient transposase recognition." @default.
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- W1503144696 date "1982-08-01" @default.
- W1503144696 modified "2023-09-25" @default.
- W1503144696 title "IS50-mediated inverse transposition" @default.
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- W1503144696 doi "https://doi.org/10.1016/0022-2836(82)90495-8" @default.
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