Matches in SemOpenAlex for { <https://semopenalex.org/work/W1503740541> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W1503740541 endingPage "11624" @default.
- W1503740541 startingPage "11619" @default.
- W1503740541 abstract "Two classes of high affinity, cGMP-specific binding sites have been found in association with a peripheral membrane protein in rod outer segments. [3H]cGMP and a photoaffinity label, 8-N3-[32P]cIMP, have been used to study these cGMP binding sites. The cGMP binding sites co-migrated with rod outer segment phosphodiesterase (EC 3.1.4.17) upon Bio-Gel A-0.5m column chromatography, sucrose density gradient centrifugation, and isoelectric focusing (pI 5.35). Upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the 8-N3-[32P]cIMP-labeled protein also migrated in a position identical with that of purified phosphodiesterase. Scatchard analysis, using purified phosphodiesterase, revealed the presence of two classes of cGMP binding sites with apparent KD values of 0.16 and 0.83 microM. A number of observations indicated that these high affinity, cGMP-specific binding sites are distinct from the phosphodiesterase catalytic site. cAMP, which is a substrate for phosphodiesterase, did not bind to the high affinity cGMP specific sites. Limited tryptic proteolysis of phosphodiesterase resulted in a striking activation of the catalytic activity and a 96% loss of cGMP binding. 1-Methyl-3-isobutylxanthine inhibited phosphodiesterase activity and enhanced the specific binding of cGMP. Mg2+ was necessary for phosphodiesterase activity, but not for high affinity cGMP binding. Finally, phosphodiesterase activity and the cGMP-specific high affinity sites showed different stabilities on storage in phosphate buffer. These specific high affinity cGMP binding sites may be involved in the regulation of phosphodiesterase activity." @default.
- W1503740541 created "2016-06-24" @default.
- W1503740541 creator A5025695582 @default.
- W1503740541 creator A5030095220 @default.
- W1503740541 creator A5030924082 @default.
- W1503740541 creator A5043612319 @default.
- W1503740541 creator A5052825834 @default.
- W1503740541 date "1980-12-01" @default.
- W1503740541 modified "2023-10-18" @default.
- W1503740541 title "Cyclic GMP-specific, high affinity, noncatalytic binding sites on light-activated phosphodiesterase." @default.
- W1503740541 cites W102592356 @default.
- W1503740541 cites W1485225530 @default.
- W1503740541 cites W1534213617 @default.
- W1503740541 cites W1538260817 @default.
- W1503740541 cites W1603066709 @default.
- W1503740541 cites W1659377994 @default.
- W1503740541 cites W1775749144 @default.
- W1503740541 cites W1974617220 @default.
- W1503740541 cites W2012644368 @default.
- W1503740541 cites W2026904289 @default.
- W1503740541 cites W2034849053 @default.
- W1503740541 cites W2035567650 @default.
- W1503740541 cites W2040955295 @default.
- W1503740541 cites W2067171842 @default.
- W1503740541 cites W2074090122 @default.
- W1503740541 cites W2075220797 @default.
- W1503740541 cites W2080565580 @default.
- W1503740541 cites W2090787116 @default.
- W1503740541 cites W2113506485 @default.
- W1503740541 cites W2147072462 @default.
- W1503740541 cites W4293247451 @default.
- W1503740541 doi "https://doi.org/10.1016/s0021-9258(19)70334-2" @default.
- W1503740541 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6254976" @default.
- W1503740541 hasPublicationYear "1980" @default.
- W1503740541 type Work @default.
- W1503740541 sameAs 1503740541 @default.
- W1503740541 citedByCount "131" @default.
- W1503740541 countsByYear W15037405412021 @default.
- W1503740541 countsByYear W15037405412023 @default.
- W1503740541 crossrefType "journal-article" @default.
- W1503740541 hasAuthorship W1503740541A5025695582 @default.
- W1503740541 hasAuthorship W1503740541A5030095220 @default.
- W1503740541 hasAuthorship W1503740541A5030924082 @default.
- W1503740541 hasAuthorship W1503740541A5043612319 @default.
- W1503740541 hasAuthorship W1503740541A5052825834 @default.
- W1503740541 hasBestOaLocation W15037405411 @default.
- W1503740541 hasConcept C12554922 @default.
- W1503740541 hasConcept C181199279 @default.
- W1503740541 hasConcept C185592680 @default.
- W1503740541 hasConcept C3020139451 @default.
- W1503740541 hasConcept C55493867 @default.
- W1503740541 hasConcept C62826618 @default.
- W1503740541 hasConcept C86803240 @default.
- W1503740541 hasConcept C95444343 @default.
- W1503740541 hasConceptScore W1503740541C12554922 @default.
- W1503740541 hasConceptScore W1503740541C181199279 @default.
- W1503740541 hasConceptScore W1503740541C185592680 @default.
- W1503740541 hasConceptScore W1503740541C3020139451 @default.
- W1503740541 hasConceptScore W1503740541C55493867 @default.
- W1503740541 hasConceptScore W1503740541C62826618 @default.
- W1503740541 hasConceptScore W1503740541C86803240 @default.
- W1503740541 hasConceptScore W1503740541C95444343 @default.
- W1503740541 hasIssue "23" @default.
- W1503740541 hasLocation W15037405411 @default.
- W1503740541 hasOpenAccess W1503740541 @default.
- W1503740541 hasPrimaryLocation W15037405411 @default.
- W1503740541 hasRelatedWork W129948102 @default.
- W1503740541 hasRelatedWork W157665363 @default.
- W1503740541 hasRelatedWork W1964542220 @default.
- W1503740541 hasRelatedWork W1999354994 @default.
- W1503740541 hasRelatedWork W2034106936 @default.
- W1503740541 hasRelatedWork W2047612150 @default.
- W1503740541 hasRelatedWork W2049480397 @default.
- W1503740541 hasRelatedWork W2078987533 @default.
- W1503740541 hasRelatedWork W2087636445 @default.
- W1503740541 hasRelatedWork W3024845111 @default.
- W1503740541 hasVolume "255" @default.
- W1503740541 isParatext "false" @default.
- W1503740541 isRetracted "false" @default.
- W1503740541 magId "1503740541" @default.
- W1503740541 workType "article" @default.