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- W1504001774 abstract "This chapter describes the development and availability of antibody reagents that, in combination with various separation techniques, can detect and quantify the intermediate peptide forms for oxytocin (OT) and vasopressin (AVP). It is suggested that it is possible to bias the epitope of the antibody to the N terminal peptide by coupling the free carboxyl group to the carrier, as was done for the VA series of OT and AVP antibodies. Because the peptide intermediates differ from one another by various charged groups, that is, free carboxyl versus amidated, additional basic amino acids (lysine or arginine), high-voltage electrophoresis (HVE) of the OT and AVP intermediates offers a rational choice for their separation. The use of HPLC separation coupled with specific RIAs provides another method for the identification and quantitation of the products of peptide processing. This methodology has proved useful in the study of the development of peptide systems in the fetal sheep." @default.
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- W1504001774 date "1995-01-01" @default.
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- W1504001774 title "[10] Immunological and related techniques for studying neurohypophyseal peptide-processing pathways" @default.
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- W1504001774 doi "https://doi.org/10.1016/s1043-9471(06)80121-7" @default.
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