Matches in SemOpenAlex for { <https://semopenalex.org/work/W1504137910> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W1504137910 abstract "Objective To study the effect of S-equol on the activation of Nrf2/ARE signaling pathway in human umbilical vein endothelial cells (HUVECs). Method HUVECs were used in this study. Dual luciferase reporter gene assay was used to determine the effect of S-equol on Nrf2-ARE-Luc activity. Total and nuclear protein were extracted, western blot assay was used to determine the effect of S-equol on Nrf2 and its downstream antioxidant gene HO-1 protein expression. Real-time PCR assay was used to detect the effect of S-equol on Nrf2 and HO-1 mRNA levels. Cells were pretreated with Znpp, the specific antagonist of HO-1 and Nrf2-siRNA, CCK-8 assay was used to determine the effect of S-equol on cell viability under oxidative stress. Results S-equol (1 nM, 10 nM and 100 nM) dose-dependently increased the activity of Nrf2-ARE-Luc. S-equol (100 nM) up-regulated Nrf2 and HO-1 protein levels, which in a dose and time-dependent in HO-1 but no in Nrf2. S-equol treatment induced Nrf2 nuclear translocation. S-equol almost had no efffect on Nrf2 mRNA level, but a dose-dependent upregulation of HO-1 mRNA levels after treatment with S-equol. Pretreatment of Znpp, the specific blocker of HO-1 and Nrf2-siRNA attenuated the protective effect of S-equol under oxidative stress in HUVECs. Conclusion Physiological concentration (nM) of S-equol activated Nrf2/ARE signaling pathway, the main antioxidant mechanism, in HUVECs, which plays a role in attenuation of S-equol in oxidative stress-induced cell injury. Grant Funding Source: Supported by The National Natural Science Foundation of China (81102129)" @default.
- W1504137910 created "2016-06-24" @default.
- W1504137910 creator A5060250549 @default.
- W1504137910 creator A5069374998 @default.
- W1504137910 creator A5081266702 @default.
- W1504137910 creator A5082703185 @default.
- W1504137910 date "2014-04-01" @default.
- W1504137910 modified "2023-09-26" @default.
- W1504137910 title "Activation of Nrf2 plays a role in the S‐equol‐mediated attenuation of oxidative stress‐induced endothelial cell injury (1146.3)" @default.
- W1504137910 doi "https://doi.org/10.1096/fasebj.28.1_supplement.1146.3" @default.
- W1504137910 hasPublicationYear "2014" @default.
- W1504137910 type Work @default.
- W1504137910 sameAs 1504137910 @default.
- W1504137910 citedByCount "0" @default.
- W1504137910 crossrefType "journal-article" @default.
- W1504137910 hasAuthorship W1504137910A5060250549 @default.
- W1504137910 hasAuthorship W1504137910A5069374998 @default.
- W1504137910 hasAuthorship W1504137910A5081266702 @default.
- W1504137910 hasAuthorship W1504137910A5082703185 @default.
- W1504137910 hasConcept C104317684 @default.
- W1504137910 hasConcept C105580179 @default.
- W1504137910 hasConcept C127561419 @default.
- W1504137910 hasConcept C134018914 @default.
- W1504137910 hasConcept C1491633281 @default.
- W1504137910 hasConcept C185592680 @default.
- W1504137910 hasConcept C202751555 @default.
- W1504137910 hasConcept C2776151105 @default.
- W1504137910 hasConcept C2776415932 @default.
- W1504137910 hasConcept C2777411675 @default.
- W1504137910 hasConcept C2778298627 @default.
- W1504137910 hasConcept C2780955279 @default.
- W1504137910 hasConcept C2781262405 @default.
- W1504137910 hasConcept C53227056 @default.
- W1504137910 hasConcept C55493867 @default.
- W1504137910 hasConcept C86803240 @default.
- W1504137910 hasConcept C95444343 @default.
- W1504137910 hasConcept C98274493 @default.
- W1504137910 hasConceptScore W1504137910C104317684 @default.
- W1504137910 hasConceptScore W1504137910C105580179 @default.
- W1504137910 hasConceptScore W1504137910C127561419 @default.
- W1504137910 hasConceptScore W1504137910C134018914 @default.
- W1504137910 hasConceptScore W1504137910C1491633281 @default.
- W1504137910 hasConceptScore W1504137910C185592680 @default.
- W1504137910 hasConceptScore W1504137910C202751555 @default.
- W1504137910 hasConceptScore W1504137910C2776151105 @default.
- W1504137910 hasConceptScore W1504137910C2776415932 @default.
- W1504137910 hasConceptScore W1504137910C2777411675 @default.
- W1504137910 hasConceptScore W1504137910C2778298627 @default.
- W1504137910 hasConceptScore W1504137910C2780955279 @default.
- W1504137910 hasConceptScore W1504137910C2781262405 @default.
- W1504137910 hasConceptScore W1504137910C53227056 @default.
- W1504137910 hasConceptScore W1504137910C55493867 @default.
- W1504137910 hasConceptScore W1504137910C86803240 @default.
- W1504137910 hasConceptScore W1504137910C95444343 @default.
- W1504137910 hasConceptScore W1504137910C98274493 @default.
- W1504137910 hasIssue "S1" @default.
- W1504137910 hasLocation W15041379101 @default.
- W1504137910 hasOpenAccess W1504137910 @default.
- W1504137910 hasPrimaryLocation W15041379101 @default.
- W1504137910 hasRelatedWork W1967059246 @default.
- W1504137910 hasRelatedWork W2345112961 @default.
- W1504137910 hasRelatedWork W2350573855 @default.
- W1504137910 hasRelatedWork W2374174892 @default.
- W1504137910 hasRelatedWork W2378204297 @default.
- W1504137910 hasRelatedWork W2390106040 @default.
- W1504137910 hasRelatedWork W2970250732 @default.
- W1504137910 hasRelatedWork W2972211998 @default.
- W1504137910 hasRelatedWork W3140047549 @default.
- W1504137910 hasRelatedWork W4294128956 @default.
- W1504137910 hasVolume "28" @default.
- W1504137910 isParatext "false" @default.
- W1504137910 isRetracted "false" @default.
- W1504137910 magId "1504137910" @default.
- W1504137910 workType "article" @default.