Matches in SemOpenAlex for { <https://semopenalex.org/work/W1504208154> ?p ?o ?g. }
- W1504208154 endingPage "165" @default.
- W1504208154 startingPage "155" @default.
- W1504208154 abstract "The ubiquitously expressed hypoxia-inducible factor-1 (HIF-1) is involved in expression of a large number of oxygen-regulated genes. HIF-1 is a heterodimer consisting of an alpha and a beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. Whereas HIF-1alpha is a novel member of this family, HIF-1beta is identical to the aryl hydrocarbon receptor nuclear translocator, previously recognized to be involved in xenobiotic metabolism. cDNA cloning revealed that mouse HIF-1alpha can be expressed as two mRNA isoforms containing alternative 5' untranslated regions and two different predicted translational start sites. We cloned and characterized 20.5 kb of the mouse HIF-1alpha gene (Hif1a) containing exon II-XV. The two alternative first exons, I.1 and I.2, are separated from exon II by approximately 24 kb and 17 kb, respectively. We also sequenced Hif1a exon I.1 and flanking regions, and mapped a single exon I.1 transcription initiation site. Reverse transcription PCR analysis of total RNA derived from normoxic and hypoxic mouse hepatoma and fibroblast cell lines suggested that the two alternative mRNA isoforms are constitutively coexpressed in these cells, and that two different promoters drive transcription of HIF-1alpha. A minimal exon I.1 promoter was identified which moderately activated heterologous gene expression, indicating that additional cis-elements are required for efficient HIF-1alpha transcription in vivo." @default.
- W1504208154 created "2016-06-24" @default.
- W1504208154 creator A5014391609 @default.
- W1504208154 creator A5015001048 @default.
- W1504208154 creator A5054613155 @default.
- W1504208154 creator A5055422167 @default.
- W1504208154 creator A5066653796 @default.
- W1504208154 creator A5089030267 @default.
- W1504208154 date "1997-05-01" @default.
- W1504208154 modified "2023-10-09" @default.
- W1504208154 title "The Mouse Gene for Hypoxia‐Inducible Factor‐1α" @default.
- W1504208154 cites W113100470 @default.
- W1504208154 cites W1503667331 @default.
- W1504208154 cites W1559318172 @default.
- W1504208154 cites W1592650154 @default.
- W1504208154 cites W1807487415 @default.
- W1504208154 cites W1937401270 @default.
- W1504208154 cites W1964037605 @default.
- W1504208154 cites W1965051773 @default.
- W1504208154 cites W1966669660 @default.
- W1504208154 cites W1968087152 @default.
- W1504208154 cites W1969455673 @default.
- W1504208154 cites W1974519880 @default.
- W1504208154 cites W1987371643 @default.
- W1504208154 cites W1988403143 @default.
- W1504208154 cites W1989451627 @default.
- W1504208154 cites W1991642534 @default.
- W1504208154 cites W1993608966 @default.
- W1504208154 cites W1994087300 @default.
- W1504208154 cites W1998340580 @default.
- W1504208154 cites W1999966058 @default.
- W1504208154 cites W2000699462 @default.
- W1504208154 cites W2004427637 @default.
- W1504208154 cites W2005561301 @default.
- W1504208154 cites W2009310436 @default.
- W1504208154 cites W2010394923 @default.
- W1504208154 cites W2015344488 @default.
- W1504208154 cites W2018153866 @default.
- W1504208154 cites W2022481764 @default.
- W1504208154 cites W2024206845 @default.
- W1504208154 cites W2032971982 @default.
- W1504208154 cites W2033553638 @default.
- W1504208154 cites W2038449972 @default.
- W1504208154 cites W2046281110 @default.
- W1504208154 cites W2046440184 @default.
- W1504208154 cites W2046794376 @default.
- W1504208154 cites W2047361593 @default.
- W1504208154 cites W2049661143 @default.
- W1504208154 cites W2050099623 @default.
- W1504208154 cites W2060449732 @default.
- W1504208154 cites W2068238193 @default.
- W1504208154 cites W2072839085 @default.
- W1504208154 cites W2078359864 @default.
- W1504208154 cites W2080032941 @default.
- W1504208154 cites W2081735518 @default.
- W1504208154 cites W2082584138 @default.
- W1504208154 cites W2084748297 @default.
- W1504208154 cites W2085609942 @default.
- W1504208154 cites W2087332917 @default.
- W1504208154 cites W2087619545 @default.
- W1504208154 cites W2100272171 @default.
- W1504208154 cites W2107867483 @default.
- W1504208154 cites W2130933110 @default.
- W1504208154 cites W2133193034 @default.
- W1504208154 cites W2133677697 @default.
- W1504208154 cites W2135836641 @default.
- W1504208154 cites W2138033100 @default.
- W1504208154 cites W2146909058 @default.
- W1504208154 cites W2159527243 @default.
- W1504208154 cites W2160099462 @default.
- W1504208154 cites W2160806267 @default.
- W1504208154 cites W2163069245 @default.
- W1504208154 cites W2163293935 @default.
- W1504208154 cites W2336116827 @default.
- W1504208154 cites W2414026282 @default.
- W1504208154 cites W2469474026 @default.
- W1504208154 cites W4294216491 @default.
- W1504208154 cites W4297692503 @default.
- W1504208154 doi "https://doi.org/10.1111/j.1432-1033.1997.t01-1-00155.x" @default.
- W1504208154 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9210478" @default.
- W1504208154 hasPublicationYear "1997" @default.
- W1504208154 type Work @default.
- W1504208154 sameAs 1504208154 @default.
- W1504208154 citedByCount "48" @default.
- W1504208154 countsByYear W15042081542012 @default.
- W1504208154 countsByYear W15042081542013 @default.
- W1504208154 countsByYear W15042081542016 @default.
- W1504208154 countsByYear W15042081542020 @default.
- W1504208154 crossrefType "journal-article" @default.
- W1504208154 hasAuthorship W1504208154A5014391609 @default.
- W1504208154 hasAuthorship W1504208154A5015001048 @default.
- W1504208154 hasAuthorship W1504208154A5054613155 @default.
- W1504208154 hasAuthorship W1504208154A5055422167 @default.
- W1504208154 hasAuthorship W1504208154A5066653796 @default.
- W1504208154 hasAuthorship W1504208154A5089030267 @default.
- W1504208154 hasBestOaLocation W15042081541 @default.
- W1504208154 hasConcept C101762097 @default.
- W1504208154 hasConcept C104317684 @default.