FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1505215090> ?p ?o ?g. }

• W1505215090 endingPage "10000" @default.
• W1505215090 startingPage "9991" @default.
• W1505215090 abstract "Rat liver glucosidase II, an endoplasmic reticulum hydrolase involved in the biosynthesis of the N-linked class of glycoproteins, has been purified in good yield to a state approaching homogeneity. The purified enzyme hydrolyzes p-nitrophenyl-alpha-D-glucopyranoside, 4-methylumbelliferyl-alpha-D-glucopyranoside, maltose, and the precursor oligosaccharides glucose1-2mannose9N-acetylglucosamine, but it does not act on glucose3mannose9N-acetylglucosamine or p-nitrophenyl-beta-D-glucopyranoside. The ratio of the rate at which glucose is released from p-nitrophenyl-alpha-D-glucopyranoside to that from glucose2mannose9N-acetylglucosamine or glucose1mannose9N-acetylglucosamine remains constant throughout the 8-step purification procedure; thus it appears that a single enzyme is responsible for the activities toward both the artificial and oligosaccharide substrates. The fact that the enzyme cleaves both of the inner 1,3-linked glucosyl residues from the precursor oligosaccharides supports the view that they are linked in the alpha-configuration. The pH dependence of enzymatic activity is quite similar for different substrates, showing a broad optimum between pH 6 and 7.5. Activity toward p-nitrophenyl-alpha-D-glucopyranoside is enhanced by 12 mM 2-deoxy-D-glucose (260-300% activation) and 25 mM mannose (150% activation), but these two compounds inhibit the action of the enzyme toward the precursor oligosaccharides. By isoelectrofocusing the purified enzyme exhibits one form, which has a pI of 3.5-3.8. Reductive polyacrylamide gel electrophoresis in sodium dodecyl sulfate indicates that glucosidase II has a subunit molecular weight of 65,000. Ferguson plot analysis of the behavior of native enzyme in polyacrylamide gels indicates that it is a 262,000-dalton tetramer. Gel filtration gives a molecular weight of 288,000. Several lines of evidence indicate that the enzyme is a glycoprotein." @default.
• W1505215090 created "2016-06-24" @default.
• W1505215090 creator A5037154031 @default.
• W1505215090 creator A5072344348 @default.
• W1505215090 date "1982-09-01" @default.
• W1505215090 modified "2023-09-30" @default.
• W1505215090 title "Purification and characterization of glucosidase II, an endoplasmic reticulum hydrolase involved in glycoprotein biosynthesis." @default.
• W1505215090 cites W139942839 @default.
• W1505215090 cites W1481175115 @default.
• W1505215090 cites W1490194415 @default.
• W1505215090 cites W1504231460 @default.
• W1505215090 cites W1510533209 @default.
• W1505215090 cites W1524547134 @default.
• W1505215090 cites W1526111678 @default.
• W1505215090 cites W1532305521 @default.
• W1505215090 cites W1547260693 @default.
• W1505215090 cites W1547340861 @default.
• W1505215090 cites W1551610456 @default.
• W1505215090 cites W1553589468 @default.
• W1505215090 cites W1569618923 @default.
• W1505215090 cites W1574058135 @default.
• W1505215090 cites W1580158514 @default.
• W1505215090 cites W1587227989 @default.
• W1505215090 cites W1592845670 @default.
• W1505215090 cites W1605414441 @default.
• W1505215090 cites W161706433 @default.
• W1505215090 cites W1698066557 @default.
• W1505215090 cites W176168377 @default.
• W1505215090 cites W1984255407 @default.
• W1505215090 cites W1986010177 @default.
• W1505215090 cites W1992252477 @default.
• W1505215090 cites W2024987855 @default.
• W1505215090 cites W2036707120 @default.
• W1505215090 cites W2038345597 @default.
• W1505215090 cites W2045840764 @default.
• W1505215090 cites W2050798509 @default.
• W1505215090 cites W2065133556 @default.
• W1505215090 cites W2080470542 @default.
• W1505215090 cites W2084554509 @default.
• W1505215090 cites W2092715189 @default.
• W1505215090 cites W2100837269 @default.
• W1505215090 cites W2111301002 @default.
• W1505215090 cites W2138032992 @default.
• W1505215090 cites W2149736282 @default.
• W1505215090 cites W2235399266 @default.
• W1505215090 cites W277110946 @default.
• W1505215090 cites W4293247451 @default.
• W1505215090 doi "https://doi.org/10.1016/s0021-9258(18)33975-9" @default.
• W1505215090 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7050114" @default.
• W1505215090 hasPublicationYear "1982" @default.
• W1505215090 type Work @default.
• W1505215090 sameAs 1505215090 @default.
• W1505215090 citedByCount "121" @default.
• W1505215090 countsByYear W15052150902013 @default.
• W1505215090 countsByYear W15052150902015 @default.
• W1505215090 countsByYear W15052150902016 @default.
• W1505215090 countsByYear W15052150902018 @default.
• W1505215090 countsByYear W15052150902021 @default.
• W1505215090 countsByYear W15052150902022 @default.
• W1505215090 crossrefType "journal-article" @default.
• W1505215090 hasAuthorship W1505215090A5037154031 @default.
• W1505215090 hasAuthorship W1505215090A5072344348 @default.
• W1505215090 hasBestOaLocation W15052150901 @default.
• W1505215090 hasConcept C108625454 @default.
• W1505215090 hasConcept C158617107 @default.
• W1505215090 hasConcept C181199279 @default.
• W1505215090 hasConcept C185592680 @default.
• W1505215090 hasConcept C2780120296 @default.
• W1505215090 hasConcept C553450214 @default.
• W1505215090 hasConcept C55493867 @default.
• W1505215090 hasConcept C86803240 @default.
• W1505215090 hasConceptScore W1505215090C108625454 @default.
• W1505215090 hasConceptScore W1505215090C158617107 @default.
• W1505215090 hasConceptScore W1505215090C181199279 @default.
• W1505215090 hasConceptScore W1505215090C185592680 @default.
• W1505215090 hasConceptScore W1505215090C2780120296 @default.
• W1505215090 hasConceptScore W1505215090C553450214 @default.
• W1505215090 hasConceptScore W1505215090C55493867 @default.
• W1505215090 hasConceptScore W1505215090C86803240 @default.
• W1505215090 hasIssue "17" @default.
• W1505215090 hasLocation W15052150901 @default.
• W1505215090 hasOpenAccess W1505215090 @default.
• W1505215090 hasPrimaryLocation W15052150901 @default.
• W1505215090 hasRelatedWork W107896380 @default.
• W1505215090 hasRelatedWork W1578965502 @default.
• W1505215090 hasRelatedWork W168461730 @default.
• W1505215090 hasRelatedWork W169735115 @default.
• W1505215090 hasRelatedWork W1992081767 @default.
• W1505215090 hasRelatedWork W2029863143 @default.
• W1505215090 hasRelatedWork W2049388301 @default.
• W1505215090 hasRelatedWork W2360317430 @default.
• W1505215090 hasRelatedWork W2801483938 @default.
• W1505215090 hasRelatedWork W950805422 @default.
• W1505215090 hasVolume "257" @default.
• W1505215090 isParatext "false" @default.
• W1505215090 isRetracted "false" @default.
• W1505215090 magId "1505215090" @default.
• W1505215090 workType "article" @default.