FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1505326511> ?p ?o ?g. }

• W1505326511 endingPage "4135" @default.
• W1505326511 startingPage "4128" @default.
• W1505326511 abstract "A rapid one-step method was developed for harvesting and concentrating insulin receptors from solubilized adipocytes, which entails precipitating soluble receptors with polyethylene glycol and resuspending the receptor-containing pellet in a reduced volume of binding buffer. With this procedure 90-100% of receptors were recovered, while 80% of cellular protein was removed, thus resulting in a marked reduction of both ligand and receptor proteases and about a 5-fold purification of the receptor. More importantly, greater than 98% of the Triton X-100 detergent was removed during this procedure so that the reduced receptor affinity observed in solubilized extracts (due to detergent) was restored to normal. Reconstituted receptors exhibited normal binding characteristics similar to those observed for plasma membrane receptors. The general utility of our receptor precipitation-reconstitution method is highlighted by studies on insulin-induced translocation of receptors from the cell-surface to the cell-interior of adipocytes and studies on the assessment of the binding affinity of nascent intracellular receptors. The results of these studies are consistent with the following. 1) Insulin initiates endocytotic uptake of insulin receptors, which then recycle back to the cell-surface. 2) Chloroquine impairs the recycling of internalized receptors while preventing receptor degradation, resulting in the progressive trapping and accumulation of receptors within cells during insulin treatment. 3) Receptor translocation during acute insulin-induced down-regulation is stoichiometric in that receptors lost from the cell-surface can be quantitatively recovered within the cell-interior. 4) In the absence of ligand, these receptors within adipocytes are mainly newly synthesized receptors enroute to the cell-surface, and they possess an affinity similar, if not identical, to mature receptors on the plasma membrane." @default.
• W1505326511 created "2016-06-24" @default.
• W1505326511 creator A5034973819 @default.
• W1505326511 creator A5048263340 @default.
• W1505326511 creator A5091424264 @default.
• W1505326511 date "1985-04-01" @default.
• W1505326511 modified "2023-09-30" @default.
• W1505326511 title "Stoichiometric translocation of adipocyte insulin receptors from the cell-surface to the cell-interior. Studies using a novel method to rapidly remove detergent and concentrate soluble receptors." @default.
• W1505326511 cites W123467347 @default.
• W1505326511 cites W1509755031 @default.
• W1505326511 cites W1552908089 @default.
• W1505326511 cites W1570060003 @default.
• W1505326511 cites W1573534810 @default.
• W1505326511 cites W1605981237 @default.
• W1505326511 cites W167100554 @default.
• W1505326511 cites W1969167744 @default.
• W1505326511 cites W1972911473 @default.
• W1505326511 cites W1981270305 @default.
• W1505326511 cites W1991010434 @default.
• W1505326511 cites W1995651320 @default.
• W1505326511 cites W2037651561 @default.
• W1505326511 cites W2042815000 @default.
• W1505326511 cites W2063128928 @default.
• W1505326511 cites W2075558536 @default.
• W1505326511 cites W2090128481 @default.
• W1505326511 cites W2106244877 @default.
• W1505326511 cites W2122556355 @default.
• W1505326511 cites W2130120676 @default.
• W1505326511 cites W2143873575 @default.
• W1505326511 cites W2150803904 @default.
• W1505326511 cites W2166354465 @default.
• W1505326511 cites W2189424535 @default.
• W1505326511 doi "https://doi.org/10.1016/s0021-9258(18)89241-9" @default.
• W1505326511 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3884606" @default.
• W1505326511 hasPublicationYear "1985" @default.
• W1505326511 type Work @default.
• W1505326511 sameAs 1505326511 @default.
• W1505326511 citedByCount "41" @default.
• W1505326511 crossrefType "journal-article" @default.
• W1505326511 hasAuthorship W1505326511A5034973819 @default.
• W1505326511 hasAuthorship W1505326511A5048263340 @default.
• W1505326511 hasAuthorship W1505326511A5091424264 @default.
• W1505326511 hasBestOaLocation W15053265111 @default.
• W1505326511 hasConcept C104317684 @default.
• W1505326511 hasConcept C112446052 @default.
• W1505326511 hasConcept C12554922 @default.
• W1505326511 hasConcept C134018914 @default.
• W1505326511 hasConcept C138626823 @default.
• W1505326511 hasConcept C1491633281 @default.
• W1505326511 hasConcept C170493617 @default.
• W1505326511 hasConcept C171089720 @default.
• W1505326511 hasConcept C185592680 @default.
• W1505326511 hasConcept C197462201 @default.
• W1505326511 hasConcept C2776175234 @default.
• W1505326511 hasConcept C2777391703 @default.
• W1505326511 hasConcept C2779306644 @default.
• W1505326511 hasConcept C55493867 @default.
• W1505326511 hasConcept C86803240 @default.
• W1505326511 hasConcept C95444343 @default.
• W1505326511 hasConceptScore W1505326511C104317684 @default.
• W1505326511 hasConceptScore W1505326511C112446052 @default.
• W1505326511 hasConceptScore W1505326511C12554922 @default.
• W1505326511 hasConceptScore W1505326511C134018914 @default.
• W1505326511 hasConceptScore W1505326511C138626823 @default.
• W1505326511 hasConceptScore W1505326511C1491633281 @default.
• W1505326511 hasConceptScore W1505326511C170493617 @default.
• W1505326511 hasConceptScore W1505326511C171089720 @default.
• W1505326511 hasConceptScore W1505326511C185592680 @default.
• W1505326511 hasConceptScore W1505326511C197462201 @default.
• W1505326511 hasConceptScore W1505326511C2776175234 @default.
• W1505326511 hasConceptScore W1505326511C2777391703 @default.
• W1505326511 hasConceptScore W1505326511C2779306644 @default.
• W1505326511 hasConceptScore W1505326511C55493867 @default.
• W1505326511 hasConceptScore W1505326511C86803240 @default.
• W1505326511 hasConceptScore W1505326511C95444343 @default.
• W1505326511 hasIssue "7" @default.
• W1505326511 hasLocation W15053265111 @default.
• W1505326511 hasOpenAccess W1505326511 @default.
• W1505326511 hasPrimaryLocation W15053265111 @default.
• W1505326511 hasRelatedWork W1495646181 @default.
• W1505326511 hasRelatedWork W1514218000 @default.
• W1505326511 hasRelatedWork W1965991580 @default.
• W1505326511 hasRelatedWork W2049070298 @default.
• W1505326511 hasRelatedWork W2082017088 @default.
• W1505326511 hasRelatedWork W2083134272 @default.
• W1505326511 hasRelatedWork W2090198044 @default.
• W1505326511 hasRelatedWork W2116514425 @default.
• W1505326511 hasRelatedWork W2145524934 @default.
• W1505326511 hasRelatedWork W2435813906 @default.
• W1505326511 hasVolume "260" @default.
• W1505326511 isParatext "false" @default.
• W1505326511 isRetracted "false" @default.
• W1505326511 magId "1505326511" @default.
• W1505326511 workType "article" @default.