Matches in SemOpenAlex for { <https://semopenalex.org/work/W1505374435> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W1505374435 endingPage "12758" @default.
- W1505374435 startingPage "12754" @default.
- W1505374435 abstract "We used a fluorescence method to measure the rate constants for the elongation of pyrene-labeled actin filaments in a number of different solvents. The absolute values of the rate constants were established by electron microscopy. Using glycerol, sucrose, or ethylene glycol to vary the solution viscosity, the association rate constant (k+) was 10(7) M-1 s-1 viscosity-1 (in centipoise). Consequently, plots of 1/k+ versus viscosity are linear and extrapolate to near the origin as expected for a diffusion-limited reaction where the rate constant approaches infinity at zero viscosity. By electron microscopy, we found that this inhibitory effect of glycerol is almost entirely at the fast growing, barbed end. For the pointed end, plots of 1/k+ versus viscosity extrapolate to a maximum rate of about 10(6) M-1 s-1 at zero viscosity, so that elongation at the pointed is not limited by diffusion. In contrast to these small molecules, polyethylene glycol, dextran, and ovalbumin all cause a concentration (and therefore viscosity)-dependent increase in k+. At any given viscosity, their effects are similar to each other. For example, at 3 centipoise, k+ = 2.2 X 10(7) M-1 s-1. We presume that this is due to an excluded volume effect that causes an increase in the thermodynamic activity of the actin. If the proteins in the cytoplasmic matrix have a similar effect, the association reactions of actin in cells may be much faster than expected from experiments done in dilute buffers." @default.
- W1505374435 created "2016-06-24" @default.
- W1505374435 creator A5032162882 @default.
- W1505374435 creator A5043478405 @default.
- W1505374435 date "1986-09-01" @default.
- W1505374435 modified "2023-10-18" @default.
- W1505374435 title "Elongation of actin filaments is a diffusion-limited reaction at the barbed end and is accelerated by inert macromolecules." @default.
- W1505374435 cites W1835632918 @default.
- W1505374435 cites W1980844900 @default.
- W1505374435 cites W1982014176 @default.
- W1505374435 cites W1987034229 @default.
- W1505374435 cites W1988377264 @default.
- W1505374435 cites W2011017251 @default.
- W1505374435 cites W2016645631 @default.
- W1505374435 cites W2020562460 @default.
- W1505374435 cites W2021539938 @default.
- W1505374435 cites W2037611941 @default.
- W1505374435 cites W2047579047 @default.
- W1505374435 cites W2070736282 @default.
- W1505374435 cites W2080068969 @default.
- W1505374435 cites W2084051721 @default.
- W1505374435 cites W2091228229 @default.
- W1505374435 cites W2115150990 @default.
- W1505374435 cites W4297917728 @default.
- W1505374435 doi "https://doi.org/10.1016/s0021-9258(18)67157-1" @default.
- W1505374435 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3745211" @default.
- W1505374435 hasPublicationYear "1986" @default.
- W1505374435 type Work @default.
- W1505374435 sameAs 1505374435 @default.
- W1505374435 citedByCount "156" @default.
- W1505374435 countsByYear W15053744352012 @default.
- W1505374435 countsByYear W15053744352013 @default.
- W1505374435 countsByYear W15053744352014 @default.
- W1505374435 countsByYear W15053744352015 @default.
- W1505374435 countsByYear W15053744352016 @default.
- W1505374435 countsByYear W15053744352017 @default.
- W1505374435 countsByYear W15053744352018 @default.
- W1505374435 countsByYear W15053744352019 @default.
- W1505374435 countsByYear W15053744352020 @default.
- W1505374435 countsByYear W15053744352021 @default.
- W1505374435 countsByYear W15053744352022 @default.
- W1505374435 countsByYear W15053744352023 @default.
- W1505374435 crossrefType "journal-article" @default.
- W1505374435 hasAuthorship W1505374435A5032162882 @default.
- W1505374435 hasAuthorship W1505374435A5043478405 @default.
- W1505374435 hasBestOaLocation W15053744351 @default.
- W1505374435 hasConcept C112950240 @default.
- W1505374435 hasConcept C121332964 @default.
- W1505374435 hasConcept C12554922 @default.
- W1505374435 hasConcept C125705527 @default.
- W1505374435 hasConcept C126348684 @default.
- W1505374435 hasConcept C154256306 @default.
- W1505374435 hasConcept C159985019 @default.
- W1505374435 hasConcept C178790620 @default.
- W1505374435 hasConcept C185592680 @default.
- W1505374435 hasConcept C192562407 @default.
- W1505374435 hasConcept C204389451 @default.
- W1505374435 hasConcept C33947775 @default.
- W1505374435 hasConcept C55493867 @default.
- W1505374435 hasConcept C69357855 @default.
- W1505374435 hasConcept C86803240 @default.
- W1505374435 hasConcept C97355855 @default.
- W1505374435 hasConceptScore W1505374435C112950240 @default.
- W1505374435 hasConceptScore W1505374435C121332964 @default.
- W1505374435 hasConceptScore W1505374435C12554922 @default.
- W1505374435 hasConceptScore W1505374435C125705527 @default.
- W1505374435 hasConceptScore W1505374435C126348684 @default.
- W1505374435 hasConceptScore W1505374435C154256306 @default.
- W1505374435 hasConceptScore W1505374435C159985019 @default.
- W1505374435 hasConceptScore W1505374435C178790620 @default.
- W1505374435 hasConceptScore W1505374435C185592680 @default.
- W1505374435 hasConceptScore W1505374435C192562407 @default.
- W1505374435 hasConceptScore W1505374435C204389451 @default.
- W1505374435 hasConceptScore W1505374435C33947775 @default.
- W1505374435 hasConceptScore W1505374435C55493867 @default.
- W1505374435 hasConceptScore W1505374435C69357855 @default.
- W1505374435 hasConceptScore W1505374435C86803240 @default.
- W1505374435 hasConceptScore W1505374435C97355855 @default.
- W1505374435 hasIssue "27" @default.
- W1505374435 hasLocation W15053744351 @default.
- W1505374435 hasOpenAccess W1505374435 @default.
- W1505374435 hasPrimaryLocation W15053744351 @default.
- W1505374435 hasRelatedWork W1846316342 @default.
- W1505374435 hasRelatedWork W1984670152 @default.
- W1505374435 hasRelatedWork W2012298134 @default.
- W1505374435 hasRelatedWork W2049384377 @default.
- W1505374435 hasRelatedWork W2063073299 @default.
- W1505374435 hasRelatedWork W2382032830 @default.
- W1505374435 hasRelatedWork W4210447090 @default.
- W1505374435 hasRelatedWork W4247320311 @default.
- W1505374435 hasRelatedWork W4249071450 @default.
- W1505374435 hasRelatedWork W2084741519 @default.
- W1505374435 hasVolume "261" @default.
- W1505374435 isParatext "false" @default.
- W1505374435 isRetracted "false" @default.
- W1505374435 magId "1505374435" @default.
- W1505374435 workType "article" @default.