FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1505954634> ?p ?o ?g. }

• W1505954634 endingPage "42" @default.
• W1505954634 startingPage "21" @default.
• W1505954634 abstract "Two processes for the production of indole alkaloids 2 l surface-immobilized bioreactor cultures of Catharanthus roseus cells using Zenk's Alkaloid Production Medium (APM) were evaluated. The 1-stage process consisted of inoculating APM containing bioreactors and incubating for 15 d. The 2-stage process involved inoculating growth medium-containing bioreactors, growing the immobilized cultures for a certain period of time and subsequently replacing this medium with APM. The production stage which lasted for 15 d. High production in 2-stage cultures required the replacement of the growth regulator 2,4-dichlorophenoxyacetic acid by indole-3-acetic acid in the growth medium and a growth stage of 6 d (late exponential phase) before production initiation. Growth, main nutrient consumption and alkaloid production were monitored. Both culture regimes resulted in similar biomass production, dw (10-13 g l-1). The 2-stage cultures yielded biomass richer in organic nutrients (200-300%) and with higher respiratory activity (approximately 250%), indicated by their lower biomass-to-carbohydrate yields (31% and 26%), as compared to 1-stage cultures (41%). Two-stage cultures produced more known products (10 as compared to 6) at yields (5 to 4800 micrograms g-1) 3 to 5 times higher than 1-stage cultures. More alkaloids were alkaloids released in the medium of 2-stage cultures, under non-lysing conditions, (20 to 4700 micrograms l-1) than in 1-stage cultures (20 to 460 micrograms l-1). These results were compared to those obtained from shake flask cultures performed at the same time, with the same C. roseus cell line and under similar regimes and reported previously. Suspension and immobilized cultures performed according to the 1-stage regime showed similar total production. However, release of known alkaloids was 2 to 3 times higher in immobilized than in suspension cultures. Total alkaloid production of 2-stage suspension cultures was 3.8-fold higher than 2-stage immobilized cultures. Two stage immobilized cultures released 4 more known alkaloids than the 2-stage suspensions. Lower oxygen availability in the 2 l immobilized cultures may explain lower specific growth rates (0.15-0.22 d-1) and total alkaloid production levels, compared to 200 ml suspension cultures (0.2-0.4 d-1) reported in our previous paper." @default.
• W1505954634 created "2016-06-24" @default.
• W1505954634 creator A5007031671 @default.
• W1505954634 creator A5022514320 @default.
• W1505954634 creator A5071213905 @default.
• W1505954634 creator A5075400497 @default.
• W1505954634 creator A5080932008 @default.
• W1505954634 date "1991-11-01" @default.
• W1505954634 modified "2023-09-27" @default.
• W1505954634 title "Effect of culture process on alkaloid production by Catharanthus roseus cells" @default.
• W1505954634 cites W1497667722 @default.
• W1505954634 cites W1589800018 @default.
• W1505954634 cites W1971154989 @default.
• W1505954634 cites W1972411494 @default.
• W1505954634 cites W1975133285 @default.
• W1505954634 cites W1979434565 @default.
• W1505954634 cites W1995951292 @default.
• W1505954634 cites W2012246177 @default.
• W1505954634 cites W2022425152 @default.
• W1505954634 cites W2037269643 @default.
• W1505954634 cites W2039236036 @default.
• W1505954634 cites W2050952101 @default.
• W1505954634 cites W2053569310 @default.
• W1505954634 cites W2067083520 @default.
• W1505954634 cites W2083594353 @default.
• W1505954634 cites W2084528521 @default.
• W1505954634 cites W2094856737 @default.
• W1505954634 cites W2135427989 @default.
• W1505954634 cites W2884032828 @default.
• W1505954634 cites W3106202904 @default.
• W1505954634 cites W4249315274 @default.
• W1505954634 doi "https://doi.org/10.1016/0168-1656(91)90259-x" @default.
• W1505954634 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1367689" @default.
• W1505954634 hasPublicationYear "1991" @default.
• W1505954634 type Work @default.
• W1505954634 sameAs 1505954634 @default.
• W1505954634 citedByCount "15" @default.
• W1505954634 countsByYear W15059546342013 @default.
• W1505954634 countsByYear W15059546342017 @default.
• W1505954634 countsByYear W15059546342018 @default.
• W1505954634 countsByYear W15059546342021 @default.
• W1505954634 countsByYear W15059546342022 @default.
• W1505954634 crossrefType "journal-article" @default.
• W1505954634 hasAuthorship W1505954634A5007031671 @default.
• W1505954634 hasAuthorship W1505954634A5022514320 @default.
• W1505954634 hasAuthorship W1505954634A5071213905 @default.
• W1505954634 hasAuthorship W1505954634A5075400497 @default.
• W1505954634 hasAuthorship W1505954634A5080932008 @default.
• W1505954634 hasConcept C100544194 @default.
• W1505954634 hasConcept C115540264 @default.
• W1505954634 hasConcept C142796444 @default.
• W1505954634 hasConcept C144027150 @default.
• W1505954634 hasConcept C168170006 @default.
• W1505954634 hasConcept C180032290 @default.
• W1505954634 hasConcept C18903297 @default.
• W1505954634 hasConcept C2776673659 @default.
• W1505954634 hasConcept C2777667214 @default.
• W1505954634 hasConcept C2779906556 @default.
• W1505954634 hasConcept C2780007248 @default.
• W1505954634 hasConcept C31903555 @default.
• W1505954634 hasConcept C55493867 @default.
• W1505954634 hasConcept C59822182 @default.
• W1505954634 hasConcept C6557445 @default.
• W1505954634 hasConcept C86803240 @default.
• W1505954634 hasConceptScore W1505954634C100544194 @default.
• W1505954634 hasConceptScore W1505954634C115540264 @default.
• W1505954634 hasConceptScore W1505954634C142796444 @default.
• W1505954634 hasConceptScore W1505954634C144027150 @default.
• W1505954634 hasConceptScore W1505954634C168170006 @default.
• W1505954634 hasConceptScore W1505954634C180032290 @default.
• W1505954634 hasConceptScore W1505954634C18903297 @default.
• W1505954634 hasConceptScore W1505954634C2776673659 @default.
• W1505954634 hasConceptScore W1505954634C2777667214 @default.
• W1505954634 hasConceptScore W1505954634C2779906556 @default.
• W1505954634 hasConceptScore W1505954634C2780007248 @default.
• W1505954634 hasConceptScore W1505954634C31903555 @default.
• W1505954634 hasConceptScore W1505954634C55493867 @default.
• W1505954634 hasConceptScore W1505954634C59822182 @default.
• W1505954634 hasConceptScore W1505954634C6557445 @default.
• W1505954634 hasConceptScore W1505954634C86803240 @default.
• W1505954634 hasIssue "1-2" @default.
• W1505954634 hasLocation W15059546341 @default.
• W1505954634 hasLocation W15059546342 @default.
• W1505954634 hasOpenAccess W1505954634 @default.
• W1505954634 hasPrimaryLocation W15059546341 @default.
• W1505954634 hasRelatedWork W1505954634 @default.
• W1505954634 hasRelatedWork W1965642671 @default.
• W1505954634 hasRelatedWork W1982643887 @default.
• W1505954634 hasRelatedWork W1988071061 @default.
• W1505954634 hasRelatedWork W1988171824 @default.
• W1505954634 hasRelatedWork W2016344956 @default.
• W1505954634 hasRelatedWork W2078929165 @default.
• W1505954634 hasRelatedWork W2163216423 @default.
• W1505954634 hasRelatedWork W2311583272 @default.
• W1505954634 hasRelatedWork W2466254428 @default.
• W1505954634 hasVolume "21" @default.
• W1505954634 isParatext "false" @default.
• W1505954634 isRetracted "false" @default.

• next