FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1506578135> ?p ?o ?g. }

• W1506578135 endingPage "6853" @default.
• W1506578135 startingPage "6841" @default.
• W1506578135 abstract "Abstract The nicotinic acetylcholine receptor has been isolated from extracts of the electric organ of the eel, Electrophorus electricus. The isolation procedure involves the following elements: (a) a rapid and reproducible assay that is based on the ability of receptor to bind cobra-[3H] neurotoxin and allows complex formation to be measured using DEAE-cellulose paper discs; (b) a series of purification steps including preparation of membrane fragments, extraction of receptors with nonionic detergents, followed by gel permeation, affinity and ion exchange chromatography. The purified receptor is isolated following 8250-fold purification with an over-all yield of approximately 5%. The receptor is a large protein that is free of ultraviolet absorbing prosthetic groups, and migrates as a single band in polyacrylamide gel electrophoresis both when native and denatured. The amino acid analysis distinguishes this protein from acetylcholinesterase. The best preparations bind 1 mole of neurotoxin per 90,000 g of protein, and the ability to form complexes is sensitive to heat and proteolysis. The molecular weight and subunit structure remain to be determined. The reaction between receptor and cobra neurotoxin proceeds toward the equilibrium state slowly. The apparent affinity of receptor for toxin increases with longer times of incubation. The toxin receptor equilibrium changes as a function of temperature, and this change is entirely due to changes in the rate of association, the dissociation rate remaining temperature independent. The kinetic and thermodynamic parameters at 25° are: k1 = 1.35 x 105 mole-1 s-1, k-1 = 1.52 x 10-4 s -1, Kd = 1.13 x 10-9 m, ΔH0 = 15.3 Cal per mole, ΔG0 = -12.2 Cal per mole, ΔS0 = +92 e.u. The toxin-receptor equilibrium and the DEAE-filter paper assay provide a convenient method for studying the interaction of receptor with cholinergic ligands." @default.
• W1506578135 created "2016-06-24" @default.
• W1506578135 creator A5006101723 @default.
• W1506578135 creator A5018051716 @default.
• W1506578135 creator A5021146956 @default.
• W1506578135 creator A5032759906 @default.
• W1506578135 creator A5063850205 @default.
• W1506578135 creator A5088568013 @default.
• W1506578135 date "1973-10-01" @default.
• W1506578135 modified "2023-10-14" @default.
• W1506578135 title "The Acetylcholine Receptor" @default.
• W1506578135 cites W1483228873 @default.
• W1506578135 cites W1597014450 @default.
• W1506578135 cites W1775749144 @default.
• W1506578135 cites W1971048984 @default.
• W1506578135 cites W1983649348 @default.
• W1506578135 cites W1988622146 @default.
• W1506578135 cites W1999099179 @default.
• W1506578135 cites W2004630889 @default.
• W1506578135 cites W2026714692 @default.
• W1506578135 cites W2034684185 @default.
• W1506578135 cites W2040461510 @default.
• W1506578135 cites W2043585912 @default.
• W1506578135 cites W2060747392 @default.
• W1506578135 cites W2070042183 @default.
• W1506578135 cites W2072323381 @default.
• W1506578135 cites W2073879684 @default.
• W1506578135 cites W2080779467 @default.
• W1506578135 cites W2084464642 @default.
• W1506578135 cites W2086123226 @default.
• W1506578135 cites W2100837269 @default.
• W1506578135 cites W2155991763 @default.
• W1506578135 cites W2162379326 @default.
• W1506578135 cites W4251600401 @default.
• W1506578135 cites W49259708 @default.
• W1506578135 doi "https://doi.org/10.1016/s0021-9258(19)43428-5" @default.
• W1506578135 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4745446" @default.
• W1506578135 hasPublicationYear "1973" @default.
• W1506578135 type Work @default.
• W1506578135 sameAs 1506578135 @default.
• W1506578135 citedByCount "204" @default.
• W1506578135 countsByYear W15065781352012 @default.
• W1506578135 countsByYear W15065781352014 @default.
• W1506578135 countsByYear W15065781352017 @default.
• W1506578135 countsByYear W15065781352020 @default.
• W1506578135 countsByYear W15065781352021 @default.
• W1506578135 crossrefType "journal-article" @default.
• W1506578135 hasAuthorship W1506578135A5006101723 @default.
• W1506578135 hasAuthorship W1506578135A5018051716 @default.
• W1506578135 hasAuthorship W1506578135A5021146956 @default.
• W1506578135 hasAuthorship W1506578135A5032759906 @default.
• W1506578135 hasAuthorship W1506578135A5063850205 @default.
• W1506578135 hasAuthorship W1506578135A5088568013 @default.
• W1506578135 hasBestOaLocation W15065781351 @default.
• W1506578135 hasConcept C116289061 @default.
• W1506578135 hasConcept C170493617 @default.
• W1506578135 hasConcept C185592680 @default.
• W1506578135 hasConcept C2775910092 @default.
• W1506578135 hasConcept C55493867 @default.
• W1506578135 hasConcept C64615621 @default.
• W1506578135 hasConcept C80161118 @default.
• W1506578135 hasConcept C86803240 @default.
• W1506578135 hasConcept C98274493 @default.
• W1506578135 hasConceptScore W1506578135C116289061 @default.
• W1506578135 hasConceptScore W1506578135C170493617 @default.
• W1506578135 hasConceptScore W1506578135C185592680 @default.
• W1506578135 hasConceptScore W1506578135C2775910092 @default.
• W1506578135 hasConceptScore W1506578135C55493867 @default.
• W1506578135 hasConceptScore W1506578135C64615621 @default.
• W1506578135 hasConceptScore W1506578135C80161118 @default.
• W1506578135 hasConceptScore W1506578135C86803240 @default.
• W1506578135 hasConceptScore W1506578135C98274493 @default.
• W1506578135 hasIssue "19" @default.
• W1506578135 hasLocation W15065781351 @default.
• W1506578135 hasOpenAccess W1506578135 @default.
• W1506578135 hasPrimaryLocation W15065781351 @default.
• W1506578135 hasRelatedWork W171626107 @default.
• W1506578135 hasRelatedWork W1988615846 @default.
• W1506578135 hasRelatedWork W2008663622 @default.
• W1506578135 hasRelatedWork W2045807167 @default.
• W1506578135 hasRelatedWork W2046592054 @default.
• W1506578135 hasRelatedWork W2092431425 @default.
• W1506578135 hasRelatedWork W2141284133 @default.
• W1506578135 hasRelatedWork W2163144454 @default.
• W1506578135 hasRelatedWork W3144716053 @default.
• W1506578135 hasRelatedWork W581368863 @default.
• W1506578135 hasVolume "248" @default.
• W1506578135 isParatext "false" @default.
• W1506578135 isRetracted "false" @default.
• W1506578135 magId "1506578135" @default.
• W1506578135 workType "article" @default.