Matches in SemOpenAlex for { <https://semopenalex.org/work/W1506652666> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W1506652666 endingPage "466" @default.
- W1506652666 startingPage "461" @default.
- W1506652666 abstract "In genetic and molecular breeding studies of plants, researchers need to design various kinds of primers based on their research purposes. So far many kinds of web- or script-based non-commercial programs for primer design are available. Because most of them do not include user interface for multipurpose usage including gene structure prediction and direct target selection on sequences, it has been a laborious work to design primers targeting on the exon or intron regions of interesting genes. Here we report a primer designing graphic user interface program, Pickprimer, that includes gene structure prediction and primer design modules by combining source codes of the Spidey and Primer3 programs. This program provides simple graphic user interface to input sequences and design primers. Genomic sequence and mRNA or coding sequence of genes can be copy and pasted or input as fasta or text files. Based on alignment of the input sequences using the Spidey module, a putative gene structure is graphically visualized along with exon-intron sequences of color codes. Primer design can be easily performed by dragging mouse on the displayed sequences or input primer targeting position with desirable values of primers. The output of designed primers with detailed information is provided by the Primer3 module. PCR evaluation of 24 selected primer sets successfully amplified single amplicons from six Brassica rapa cultivars. The Pickprimer will be a convenient tool for genetic and molecular breeding studies of plants." @default.
- W1506652666 created "2016-06-24" @default.
- W1506652666 creator A5026908790 @default.
- W1506652666 creator A5066865922 @default.
- W1506652666 creator A5071702186 @default.
- W1506652666 creator A5082580461 @default.
- W1506652666 date "2011-01-01" @default.
- W1506652666 modified "2023-09-26" @default.
- W1506652666 title "Pickprimer: A Graphic User Interface Program for Primer Design on the Gene Target Region" @default.
- W1506652666 cites W1749816123 @default.
- W1506652666 cites W1991330646 @default.
- W1506652666 cites W2100303227 @default.
- W1506652666 cites W2106882534 @default.
- W1506652666 cites W2113745578 @default.
- W1506652666 cites W2130063668 @default.
- W1506652666 cites W2138782170 @default.
- W1506652666 cites W2148277646 @default.
- W1506652666 cites W2155649556 @default.
- W1506652666 cites W2157748357 @default.
- W1506652666 cites W2170488656 @default.
- W1506652666 hasPublicationYear "2011" @default.
- W1506652666 type Work @default.
- W1506652666 sameAs 1506652666 @default.
- W1506652666 citedByCount "1" @default.
- W1506652666 crossrefType "journal-article" @default.
- W1506652666 hasAuthorship W1506652666A5026908790 @default.
- W1506652666 hasAuthorship W1506652666A5066865922 @default.
- W1506652666 hasAuthorship W1506652666A5071702186 @default.
- W1506652666 hasAuthorship W1506652666A5082580461 @default.
- W1506652666 hasConcept C104317684 @default.
- W1506652666 hasConcept C113843644 @default.
- W1506652666 hasConcept C178790620 @default.
- W1506652666 hasConcept C185592680 @default.
- W1506652666 hasConcept C2777563447 @default.
- W1506652666 hasConcept C36823959 @default.
- W1506652666 hasConcept C41008148 @default.
- W1506652666 hasConcept C49105822 @default.
- W1506652666 hasConcept C54355233 @default.
- W1506652666 hasConcept C55352822 @default.
- W1506652666 hasConcept C55493867 @default.
- W1506652666 hasConcept C58226133 @default.
- W1506652666 hasConcept C70721500 @default.
- W1506652666 hasConcept C8185291 @default.
- W1506652666 hasConcept C86803240 @default.
- W1506652666 hasConcept C91779695 @default.
- W1506652666 hasConcept C94671646 @default.
- W1506652666 hasConceptScore W1506652666C104317684 @default.
- W1506652666 hasConceptScore W1506652666C113843644 @default.
- W1506652666 hasConceptScore W1506652666C178790620 @default.
- W1506652666 hasConceptScore W1506652666C185592680 @default.
- W1506652666 hasConceptScore W1506652666C2777563447 @default.
- W1506652666 hasConceptScore W1506652666C36823959 @default.
- W1506652666 hasConceptScore W1506652666C41008148 @default.
- W1506652666 hasConceptScore W1506652666C49105822 @default.
- W1506652666 hasConceptScore W1506652666C54355233 @default.
- W1506652666 hasConceptScore W1506652666C55352822 @default.
- W1506652666 hasConceptScore W1506652666C55493867 @default.
- W1506652666 hasConceptScore W1506652666C58226133 @default.
- W1506652666 hasConceptScore W1506652666C70721500 @default.
- W1506652666 hasConceptScore W1506652666C8185291 @default.
- W1506652666 hasConceptScore W1506652666C86803240 @default.
- W1506652666 hasConceptScore W1506652666C91779695 @default.
- W1506652666 hasConceptScore W1506652666C94671646 @default.
- W1506652666 hasIssue "5" @default.
- W1506652666 hasLocation W15066526661 @default.
- W1506652666 hasOpenAccess W1506652666 @default.
- W1506652666 hasPrimaryLocation W15066526661 @default.
- W1506652666 hasRelatedWork W1580859948 @default.
- W1506652666 hasRelatedWork W2006355997 @default.
- W1506652666 hasRelatedWork W2060708446 @default.
- W1506652666 hasRelatedWork W2062837212 @default.
- W1506652666 hasRelatedWork W2066775373 @default.
- W1506652666 hasRelatedWork W2078028348 @default.
- W1506652666 hasRelatedWork W2105611495 @default.
- W1506652666 hasRelatedWork W2113866066 @default.
- W1506652666 hasRelatedWork W2126932953 @default.
- W1506652666 hasRelatedWork W2142966824 @default.
- W1506652666 hasRelatedWork W2143421305 @default.
- W1506652666 hasRelatedWork W2146056253 @default.
- W1506652666 hasRelatedWork W2161698191 @default.
- W1506652666 hasRelatedWork W2187847575 @default.
- W1506652666 hasRelatedWork W2408872048 @default.
- W1506652666 hasRelatedWork W2465485427 @default.
- W1506652666 hasRelatedWork W2943577714 @default.
- W1506652666 hasRelatedWork W2763194105 @default.
- W1506652666 hasRelatedWork W2926712253 @default.
- W1506652666 hasRelatedWork W3150415215 @default.
- W1506652666 hasVolume "29" @default.
- W1506652666 isParatext "false" @default.
- W1506652666 isRetracted "false" @default.
- W1506652666 magId "1506652666" @default.
- W1506652666 workType "article" @default.