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- W150670586 abstract "The present study evaluates the effects of various loadingsolutions, concentrations of glycerol and vitrification solutions and theirtime of exposure on the vitrification of rambutan embryos in liquidnitrogen.In the initial study to evaluate the effects of loading solutions onsurvival, excised embryos were exposed to four loading solutions. Thetwo most promising loading solutions were LB (1.5 M glycerol + 0.4 Msucrose + 5% DMSO), which gave 44.0 % viability and 32.4 % survivaland LA (2.0 M glycerol + 0.4 M sucrose) which gave 39.3 % viability and28.1 % survival after freezing.The effects of different concentrations of glycerol (0 - 2.0 M) inthe most promising loading solutions were evaluated further. For loadingsolution LB, 1.5 M glycerol gave highest survival of 22.7 %. For loading solution LS, 1.5 M glycerol gave highest survival of 22.7 %. For loadingsolution LA, 0 M glycerol or the use of only 0.4 M sucrose gave thehighest viability of 76.0 % and survival of 59.0 %. Hence, loadingsolution with only 0.4 M sucrose (LA without glycerol) was established inthis study as the most effective loading solution for rambutan embryos.The effects of exposure time (0-16 hours) to the best loadingsolution on survival of rambutan embryos were further investigated. Itwas found that 8 hours duration gave the highest viability (47.7 %) andsurvival (32.8 %).Having confirmed the best loading treatment, the study furtherevaluates the effects of six vitrification solutions on survival of rambutanembryos in liquid nitrogen. The results show that after freezing,L Solution gave the highest viability (46.0 %) and survival (24.0 %).L Solution was therefore selected as the most effective vitrificationsolution.In optimizing the time of exposure, excised rambutan embryoswere exposed to L Solution for 0 to 90 minutes before LN exposure. Thehighest viability (55.6 %) and survival (40.3 %) after vitirification wereachieved at 60 minutes exposure. Longer exposure to L Solution for upto 90 minutes reduced survival to 16.0 %." @default.
- W150670586 created "2016-06-24" @default.
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- W150670586 date "2001-01-01" @default.
- W150670586 modified "2023-09-27" @default.
- W150670586 title "Cryopreservation of Excised Embryos of Rambutan (Nephelium Lappaceum L.) Using Vitrification Technique" @default.
- W150670586 hasPublicationYear "2001" @default.
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