Matches in SemOpenAlex for { <https://semopenalex.org/work/W1506813767> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W1506813767 endingPage "240" @default.
- W1506813767 startingPage "227" @default.
- W1506813767 abstract "Trichoplusia ni capsules were purified by a combination of differential and ratezonal centrifugation on 50 to 80% sucrose gradients. The virus was released and purified from the inclusion body protein by exposure to weak alkali and subsequent centrifugation on 10 to 40% sucrose gradients. Virus preparations maintained in 0.01 m phosphate buffer (pH, 7.8) demonstrated 3 sedimenting forms on 10 to 40% sucrose gradients which were designated as A and B (with outer envelope) and Y (without outer envelope). The virus was unstable in 0.01 m phosphate buffer pH 7.8, and had a tendency to aggregate, and/or dissociate into the outer and inner membranes and a top fraction. The infection properties of each virus form and the top fraction were tested. Other buffers or additives did not change these phenomena. These virus forms and components were separated by a combination of sedimentations on 10 to 40% and 5 to 20% sucrose gradients and their ultrastructure confirmed by electron microscopy. Solutions containing virus forms A and B exhibited an E260E280 of 1.26 and DNA and protein concentrations of 27.4 and 28.8 μg DNA/E260 and 105 and 109 μg protein/E260 for forms A and B, respectively. Preparations of the Y form contained 73.0 μg DNA/E260 and 258 μg protein/E260, but exhibited only 53% the ultraviolet extinction of virus forms A and B. Studies confirmed that the granulosis virus of Trichoplusia ni is ultrastructurally similar to other granuloses." @default.
- W1506813767 created "2016-06-24" @default.
- W1506813767 creator A5052819894 @default.
- W1506813767 creator A5067532303 @default.
- W1506813767 date "1970-09-01" @default.
- W1506813767 modified "2023-10-03" @default.
- W1506813767 title "Alkali-liberated granulosis virus of Trichoplusia ni" @default.
- W1506813767 cites W1595199770 @default.
- W1506813767 cites W1775749144 @default.
- W1506813767 cites W1975176230 @default.
- W1506813767 cites W1978719589 @default.
- W1506813767 cites W1989664105 @default.
- W1506813767 cites W2010024873 @default.
- W1506813767 cites W2030639097 @default.
- W1506813767 cites W203704018 @default.
- W1506813767 cites W2040948855 @default.
- W1506813767 cites W2092974548 @default.
- W1506813767 cites W2118543320 @default.
- W1506813767 cites W2138851406 @default.
- W1506813767 cites W978299559 @default.
- W1506813767 doi "https://doi.org/10.1016/0022-2011(70)90064-9" @default.
- W1506813767 hasPublicationYear "1970" @default.
- W1506813767 type Work @default.
- W1506813767 sameAs 1506813767 @default.
- W1506813767 citedByCount "36" @default.
- W1506813767 countsByYear W15068137672015 @default.
- W1506813767 countsByYear W15068137672017 @default.
- W1506813767 countsByYear W15068137672020 @default.
- W1506813767 crossrefType "journal-article" @default.
- W1506813767 hasAuthorship W1506813767A5052819894 @default.
- W1506813767 hasAuthorship W1506813767A5067532303 @default.
- W1506813767 hasConcept C132946820 @default.
- W1506813767 hasConcept C153911025 @default.
- W1506813767 hasConcept C159047783 @default.
- W1506813767 hasConcept C173758957 @default.
- W1506813767 hasConcept C2522874641 @default.
- W1506813767 hasConcept C2778350275 @default.
- W1506813767 hasConcept C2778597767 @default.
- W1506813767 hasConcept C2779341487 @default.
- W1506813767 hasConcept C55493867 @default.
- W1506813767 hasConcept C59822182 @default.
- W1506813767 hasConcept C7623868 @default.
- W1506813767 hasConcept C86803240 @default.
- W1506813767 hasConcept C87555872 @default.
- W1506813767 hasConceptScore W1506813767C132946820 @default.
- W1506813767 hasConceptScore W1506813767C153911025 @default.
- W1506813767 hasConceptScore W1506813767C159047783 @default.
- W1506813767 hasConceptScore W1506813767C173758957 @default.
- W1506813767 hasConceptScore W1506813767C2522874641 @default.
- W1506813767 hasConceptScore W1506813767C2778350275 @default.
- W1506813767 hasConceptScore W1506813767C2778597767 @default.
- W1506813767 hasConceptScore W1506813767C2779341487 @default.
- W1506813767 hasConceptScore W1506813767C55493867 @default.
- W1506813767 hasConceptScore W1506813767C59822182 @default.
- W1506813767 hasConceptScore W1506813767C7623868 @default.
- W1506813767 hasConceptScore W1506813767C86803240 @default.
- W1506813767 hasConceptScore W1506813767C87555872 @default.
- W1506813767 hasIssue "2" @default.
- W1506813767 hasLocation W15068137671 @default.
- W1506813767 hasOpenAccess W1506813767 @default.
- W1506813767 hasPrimaryLocation W15068137671 @default.
- W1506813767 hasRelatedWork W146430892 @default.
- W1506813767 hasRelatedWork W1972636959 @default.
- W1506813767 hasRelatedWork W2018102719 @default.
- W1506813767 hasRelatedWork W2019582563 @default.
- W1506813767 hasRelatedWork W2048241441 @default.
- W1506813767 hasRelatedWork W2104831898 @default.
- W1506813767 hasRelatedWork W2121121557 @default.
- W1506813767 hasRelatedWork W2164921357 @default.
- W1506813767 hasRelatedWork W2334913511 @default.
- W1506813767 hasRelatedWork W2784281153 @default.
- W1506813767 hasVolume "16" @default.
- W1506813767 isParatext "false" @default.
- W1506813767 isRetracted "false" @default.
- W1506813767 magId "1506813767" @default.
- W1506813767 workType "article" @default.