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• W1507468262 endingPage "1956" @default.
• W1507468262 startingPage "1939" @default.
• W1507468262 abstract "To provide a basis for the development of approaches to treat elastin‐degrading diseases, the aim of this study was to investigate the degradation of the natural substrate tropoelastin by the elastinolytic matrix metalloproteinases MMP‐7, MMP‐9, and MMP‐12 and to compare the cleavage site specificities of the enzymes using complementary MS techniques and molecular modeling. Furthermore, the ability of the three proteases to release bioactive peptides was studied. Tropoelastin was readily degraded by all three MMPs. Eighty‐nine cleavage sites in tropoelastin were identified for MMP‐12, whereas MMP‐7 and MMP‐9 were found to cleave at only 58 and 63 sites, respectively. Cleavages occurred predominantly in the N‐terminal and C‐terminal regions of tropoelastin. With respect to the cleavage site specificities, the study revealed that all three MMPs similarly tolerate hydrophobic and/or aliphatic amino acids, including Pro, Gly, Ile, and Val, at P 1 ′. MMP‐7 shows a strong preference for Leu at P 1 ′, which is also well accepted by MMP‐9 and MMP‐12. Of all three MMPs, MMP‐12 best tolerates bulky charged and aromatic amino acids at P 1 ′. All three MMPs showed a clear preference for Pro at P 3 that could be structurally explained by molecular modeling. Analysis of the generated peptides revealed that all three MMPs show a similar ability to release bioactive sequences, with MMP‐12 producing the highest number of these peptides. Furthermore, the generated peptides YTTGKLPYGYGPGG, YGARPGVGVGGIP, and PGFGAVPGA, containing GxxPG motifs that have not yet been proven to be bioactive, were identified as new matrikines upon biological activity testing. Structured digital abstract MINT‐7709630 : MMP‐7 (uniprotkb: P09237 ) cleaves ( MI:0194 ) Tropoelastin (uniprotkb: P15502 ) by protease assay ( MI:0435 ) MINT‐7709668 : MMP‐9 (uniprotkb: P14780 ) cleaves ( MI:0194 ) Tropoelastin (uniprotkb: P15502 ) by protease assay ( MI:0435 ) MINT‐7709289 : MMP‐12 (uniprotkb: P39900 ) cleaves ( MI:0194 ) Tropoelastin (uniprotkb: P15502 ) by protease assay ( MI:0435 )" @default.
• W1507468262 created "2016-06-24" @default.
• W1507468262 creator A5007081664 @default.
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• W1507468262 date "2010-03-30" @default.
• W1507468262 modified "2023-10-18" @default.
• W1507468262 title "Degradation of tropoelastin by matrix metalloproteinases - cleavage site specificities and release of matrikines" @default.
• W1507468262 cites W117109844 @default.
• W1507468262 cites W127344325 @default.
• W1507468262 cites W1561068150 @default.
• W1507468262 cites W1565388169 @default.
• W1507468262 cites W1604907834 @default.
• W1507468262 cites W1844893699 @default.
• W1507468262 cites W1872370876 @default.
• W1507468262 cites W1935587632 @default.
• W1507468262 cites W1968505541 @default.
• W1507468262 cites W1969749903 @default.
• W1507468262 cites W1971887998 @default.
• W1507468262 cites W1972038244 @default.
• W1507468262 cites W1974268074 @default.
• W1507468262 cites W1974680421 @default.
• W1507468262 cites W1978330686 @default.
• W1507468262 cites W1981593008 @default.
• W1507468262 cites W1983247913 @default.
• W1507468262 cites W1984106304 @default.
• W1507468262 cites W1985469117 @default.
• W1507468262 cites W1988312910 @default.
• W1507468262 cites W1990022756 @default.
• W1507468262 cites W1991321262 @default.
• W1507468262 cites W1991890343 @default.
• W1507468262 cites W1993863650 @default.
• W1507468262 cites W1995229312 @default.
• W1507468262 cites W1995539112 @default.
• W1507468262 cites W1995790371 @default.
• W1507468262 cites W2000044234 @default.
• W1507468262 cites W2007441851 @default.
• W1507468262 cites W2011191553 @default.
• W1507468262 cites W2011475954 @default.
• W1507468262 cites W2013214083 @default.
• W1507468262 cites W2014280470 @default.
• W1507468262 cites W2017517432 @default.
• W1507468262 cites W2017957433 @default.
• W1507468262 cites W2023395548 @default.
• W1507468262 cites W2027580557 @default.
• W1507468262 cites W2030578080 @default.
• W1507468262 cites W2031304251 @default.
• W1507468262 cites W2032962964 @default.
• W1507468262 cites W2032971072 @default.
• W1507468262 cites W2034752268 @default.
• W1507468262 cites W2034799822 @default.
• W1507468262 cites W2046007528 @default.
• W1507468262 cites W2046091872 @default.
• W1507468262 cites W2046480038 @default.
• W1507468262 cites W2047466993 @default.
• W1507468262 cites W2050198557 @default.
• W1507468262 cites W2050655183 @default.
• W1507468262 cites W2051667852 @default.
• W1507468262 cites W2063699934 @default.
• W1507468262 cites W2068608850 @default.
• W1507468262 cites W2071092166 @default.
• W1507468262 cites W2072570417 @default.
• W1507468262 cites W2075990016 @default.
• W1507468262 cites W2078410720 @default.
• W1507468262 cites W2080553010 @default.
• W1507468262 cites W2080803003 @default.
• W1507468262 cites W2085245182 @default.
• W1507468262 cites W2085320561 @default.
• W1507468262 cites W2090494279 @default.
• W1507468262 cites W2093074688 @default.
• W1507468262 cites W2097582641 @default.
• W1507468262 cites W2099969611 @default.
• W1507468262 cites W2103868159 @default.
• W1507468262 cites W2110667456 @default.
• W1507468262 cites W2115205753 @default.
• W1507468262 cites W2116431176 @default.
• W1507468262 cites W2116717938 @default.
• W1507468262 cites W2117211947 @default.
• W1507468262 cites W2117960153 @default.
• W1507468262 cites W2118233996 @default.
• W1507468262 cites W2119523001 @default.
• W1507468262 cites W2124892358 @default.
• W1507468262 cites W2128049766 @default.
• W1507468262 cites W2129610386 @default.
• W1507468262 cites W2131093431 @default.
• W1507468262 cites W2134652066 @default.
• W1507468262 cites W2148147384 @default.
• W1507468262 cites W2154401382 @default.
• W1507468262 cites W2164692123 @default.
• W1507468262 cites W2165967197 @default.

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