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• W1507486004 abstract "Keyhole limpet hemocyanin (KLH)-primed lymph node cell (LNC) populations were incubated with various amounts of KLH and the cellular incorporation of tritiated thymidine ([3H]TdR) or tritiated N6, O2′ dibutyryl cyclic AMP ([3H]DbcAMP) was determined. T LNC responded more vigorously than did complement receptor lymphocytes (CRL), i.e., B cells, at all KLH concentrations, during all time intervals examined, and in the presence or absence of normal rabbit serum (NRS). The depletion of adherent cells from KLH-primed LNC resulted in no significant decrease in KLH-induced incorporation of either [3H]TdR or [3H]DbcAMP in any of the LNC populations. Thus it appeared that variation among LNC populations in the incidence of macrophages did not account for the marked variation in their responses. Cultures containing equal numbers of T and CRL were induced to incorporate more [3H]TdR or [3H]DbcAMP than either population cultured separately or the sum of their individual responses. It was concluded that KLH-induced incorporation of these substances into primed, isolated LNC, was primarily manifested in the T-cell population. The synergism seen in cultures containing mixtures of T and CRL suggested that B cells are induced to incorporate [3H]TdR or [3H]DbcAMP in the presence of antigen and T-cell product(s). KLH-induced incorporation of [3H]TdR into KLH-primed LNC was inhibited by cholera enterotoxin (CT) and DbcAMP as previously reported. However, CT or DbcAMP inhibited this incorporation into T LNC to a greater extent than into CRL or unfractionated LNC." @default.
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• W1507486004 date "1979-03-01" @default.
• W1507486004 modified "2023-09-25" @default.
• W1507486004 title "Preparation, characterization, and functions of rabbit lymph node populations" @default.
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• W1507486004 doi "https://doi.org/10.1016/0008-8749(79)90156-4" @default.
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