Matches in SemOpenAlex for { <https://semopenalex.org/work/W1507543630> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W1507543630 endingPage "5191" @default.
- W1507543630 startingPage "5185" @default.
- W1507543630 abstract "Two putP mutants of Escherichia coli K-12 that were defective in proline transport but retained the binding activities of the major proline carrier were isolated (T. Mogi, H. Yamamoto, T. Nakao, I. Yamato, and Y. Anraku, Mol. Gen. Genet. 202:35-41, 1986). One of these mutations and three null-type mutations (K. Motojima, I. Yamato, and Y. Anraku, J. Bacteriol. 136:5-9, 1978) were cloned into a pBR322 putP+ hybrid plasmid (pTMP5) by in vivo recombination. Cytoplasmic membrane vesicles were prepared from the mutant strains and strains harboring pTMP5 putP plasmids, and the properties of the proline-binding reaction of the mutant putP carriers in membranes were examined under nonenergized conditions. The putP19, putP21, and putP22 mutations, which were mapped in the same DNA segment of the putP gene (Mogi et al., Mol. Gen. Genet. 202:35-41, 1986), caused the complete loss of proline carrier activity. The proline carriers encoded by the mutant putP genes, putP9 and putP32, and putP32 in pTMP5-32, which was derived from in vivo recombination with the putP32 mutation, had altered sodium ion and proton dependence of binding affinities for proline and were resistant to N-ethylmaleimide inactivation without changes in the specificities for substrates and alkaline metal cations. The nucleotide sequence of the putP32 lesion located on the 0.35-megadalton RsaI-PvuII fragment in the putP gene in pTMP5-32 was determined; the mutation changed a cytosine at position 1001 to a thymine, causing the alteration of arginine to cysteine at amino acid position 257 in the primary structure of the proline carrier. It was shown that this one point mutation was enough to produce the phenotype of pTMP5-32 by in vitro DNA replacement of the AcyI-PvuII fragment of the wild-type putP gene with the DNA fragment containing the mutated nucleotide sequence." @default.
- W1507543630 created "2016-06-24" @default.
- W1507543630 creator A5015432645 @default.
- W1507543630 creator A5044805307 @default.
- W1507543630 creator A5051850457 @default.
- W1507543630 creator A5060022930 @default.
- W1507543630 creator A5072481430 @default.
- W1507543630 date "1988-11-01" @default.
- W1507543630 modified "2023-10-02" @default.
- W1507543630 title "Proline carrier mutant of Escherichia coli K-12 with altered cation sensitivity of substrate-binding activity: cloning, biochemical characterization, and identification of the mutation" @default.
- W1507543630 cites W1037383866 @default.
- W1507543630 cites W1488898406 @default.
- W1507543630 cites W1492365338 @default.
- W1507543630 cites W1504070555 @default.
- W1507543630 cites W1567963927 @default.
- W1507543630 cites W1591918815 @default.
- W1507543630 cites W1606707893 @default.
- W1507543630 cites W1849997836 @default.
- W1507543630 cites W1886339036 @default.
- W1507543630 cites W1949490448 @default.
- W1507543630 cites W1970131469 @default.
- W1507543630 cites W1983448230 @default.
- W1507543630 cites W2004125443 @default.
- W1507543630 cites W2019410656 @default.
- W1507543630 cites W2043755841 @default.
- W1507543630 cites W2045284490 @default.
- W1507543630 cites W2045676450 @default.
- W1507543630 cites W2048468042 @default.
- W1507543630 cites W2050599642 @default.
- W1507543630 cites W2070804112 @default.
- W1507543630 cites W2077320775 @default.
- W1507543630 cites W2077478707 @default.
- W1507543630 cites W2087392596 @default.
- W1507543630 doi "https://doi.org/10.1128/jb.170.11.5185-5191.1988" @default.
- W1507543630 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/211588" @default.
- W1507543630 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3053649" @default.
- W1507543630 hasPublicationYear "1988" @default.
- W1507543630 type Work @default.
- W1507543630 sameAs 1507543630 @default.
- W1507543630 citedByCount "24" @default.
- W1507543630 countsByYear W15075436302015 @default.
- W1507543630 crossrefType "journal-article" @default.
- W1507543630 hasAuthorship W1507543630A5015432645 @default.
- W1507543630 hasAuthorship W1507543630A5044805307 @default.
- W1507543630 hasAuthorship W1507543630A5051850457 @default.
- W1507543630 hasAuthorship W1507543630A5060022930 @default.
- W1507543630 hasAuthorship W1507543630A5072481430 @default.
- W1507543630 hasBestOaLocation W15075436301 @default.
- W1507543630 hasConcept C104317684 @default.
- W1507543630 hasConcept C143065580 @default.
- W1507543630 hasConcept C153911025 @default.
- W1507543630 hasConcept C2779815552 @default.
- W1507543630 hasConcept C515207424 @default.
- W1507543630 hasConcept C54355233 @default.
- W1507543630 hasConcept C547475151 @default.
- W1507543630 hasConcept C55493867 @default.
- W1507543630 hasConcept C86803240 @default.
- W1507543630 hasConceptScore W1507543630C104317684 @default.
- W1507543630 hasConceptScore W1507543630C143065580 @default.
- W1507543630 hasConceptScore W1507543630C153911025 @default.
- W1507543630 hasConceptScore W1507543630C2779815552 @default.
- W1507543630 hasConceptScore W1507543630C515207424 @default.
- W1507543630 hasConceptScore W1507543630C54355233 @default.
- W1507543630 hasConceptScore W1507543630C547475151 @default.
- W1507543630 hasConceptScore W1507543630C55493867 @default.
- W1507543630 hasConceptScore W1507543630C86803240 @default.
- W1507543630 hasIssue "11" @default.
- W1507543630 hasLocation W15075436301 @default.
- W1507543630 hasLocation W15075436302 @default.
- W1507543630 hasLocation W15075436303 @default.
- W1507543630 hasLocation W15075436304 @default.
- W1507543630 hasOpenAccess W1507543630 @default.
- W1507543630 hasPrimaryLocation W15075436301 @default.
- W1507543630 hasRelatedWork W1606982276 @default.
- W1507543630 hasRelatedWork W1830352000 @default.
- W1507543630 hasRelatedWork W2064207257 @default.
- W1507543630 hasRelatedWork W2075402811 @default.
- W1507543630 hasRelatedWork W2080910126 @default.
- W1507543630 hasRelatedWork W2090064233 @default.
- W1507543630 hasRelatedWork W2131114457 @default.
- W1507543630 hasRelatedWork W2156674132 @default.
- W1507543630 hasRelatedWork W2180148536 @default.
- W1507543630 hasRelatedWork W86974628 @default.
- W1507543630 hasVolume "170" @default.
- W1507543630 isParatext "false" @default.
- W1507543630 isRetracted "false" @default.
- W1507543630 magId "1507543630" @default.
- W1507543630 workType "article" @default.