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- W1507544034 abstract "This paper discusses the influence of various detergents, pH values, and ionic strengths on the rate of oxygen uptake (normalized in units of J,Jmol oxygenl hrl mg chlorophyll a) in photosensitive solutions containing in vitro photosystem I and cytochrome Css3, obtained from the cyanobacterium, Thermosynechococcus elongatus. Specifically, three experimental studies were conducted for the determination of oxygen uptake rate with respect to: detergent type, hydronium ion concentration, and monovalent ion concentration in PSI/cytochrome Css3 solutions. Experimental solutions contained either MES, HEPES, or Tris buffers, Triton X-100 or OM detergents, PSI purified in Triton X100 or OM detergents, the presence or lack of cytochrome Css3, methyl viologen, and sodium ascorbate. Aliquots of each solution were tested via Clark electrodes to determine oxygen uptake rate via methyl viologen as the electron acceptor for PSI. This rate is a function of the affinity of cytochrome Cs53 for the docking site on PSI. In addition to the effect of pH and ionic strength on the reduction rate, two different detergents (Triton X-100 and OM) were used to stabilize PSI in solution after purification and their effects on the rate were studied. Ultimately, the goal of this series of experiments was to determine optimal conditions of pH, ionic strength, and detergent addition for the reduction of PSI by cytochrome Cs53. INTRODUCTION The mechanism of oxygen uptake begins with photosystem I (PSI), a trimeric protein (Figure 1) of the thylakoid membrane of photosynthetic organisms, whose groups form a reaction center (1). When a photon strikes P700 (Figure 2), a dimer of chlorophyll a, an Figure 1: PSI. electron from PSI becomes excited with energy (2). In the presence of an electron acceptor, such as aqueous methyl viologen, PSI reduces the acceptor, thereby depleting itself of an electron. The electron lost by PSI to methyl viologen can be replaced by a number of electron donors. Cytochrome c, a protein bound to iron (2), and sodium ascorbate have been used as electron donors to PSI in preceeding Transfer of energy Electron transfer Photoayatem I I ~Reaction ReactionI center center I chlorophyll) ionizable p690 p700 Antenna pigment molecules Figure 2: Electron transfer. experimental studies due to the relative ease with which cytochrome c can be produced" @default.
- W1507544034 created "2016-06-24" @default.
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- W1507544034 date "2008-01-01" @default.
- W1507544034 modified "2023-09-25" @default.
- W1507544034 title "Probing the Effects of Detergent, pH and lonic Strength" @default.
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