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- W1507604306 abstract "This chapter reviews the general transcription factor transcription Factor II D (TFIID), playing a key role in the regulation of gene expression by RNA polymerase II. In yeast, the TFIID complex is composed of the TATA box-binding protein (TBP) plus 14 TBP-associated factors (TAFs). Roles of the TAF proteins in transcription have been examined by inactivating TAF function and monitoring the effects on gene expression. Conditional mutants must be isolated and employed in their analysis, as all but one of the subunit of the TFIID complex is essential for the viability in yeast. The chapter explores the conditional depletion/expression strategies used in the systems that place the TAF gene under the control of a tightly regulated promoter. The advantage of this strategy is that the protein is depleted from the cell, alleviating concerns that the gene function is partially inactivated." @default.
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- W1507604306 date "2003-01-01" @default.
- W1507604306 modified "2023-09-23" @default.
- W1507604306 title "Functional Analysis of TFIID Components Using Conditional Mutants" @default.
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- W1507604306 doi "https://doi.org/10.1016/s0076-6879(03)70036-6" @default.
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