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• W150762014 abstract "Research Article1 April 1990free access The IS10 antisense RNA blocks ribosome binding at the transposase translation initiation site. C. Ma C. Ma Molecular Biology Institute, University of California, Los Angeles 90024. Search for more papers by this author R.W. Simons R.W. Simons Molecular Biology Institute, University of California, Los Angeles 90024. Search for more papers by this author C. Ma C. Ma Molecular Biology Institute, University of California, Los Angeles 90024. Search for more papers by this author R.W. Simons R.W. Simons Molecular Biology Institute, University of California, Los Angeles 90024. Search for more papers by this author Author Information C. Ma1 and R.W. Simons1 1Molecular Biology Institute, University of California, Los Angeles 90024. The EMBO Journal (1990)9:1267-1274https://doi.org/10.1002/j.1460-2075.1990.tb08235.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Transposase (tnp) expression from insertion sequence IS10 is controlled, in part, by an antisense RNA, RNA-OUT, which pairs to the translation initiation region of the tnp mRNA, RNA-IN. Genetic experiments suggest that control occurs post-transcriptionally. Here, we present evidence that bears on the control mechanism. Specific ribosome binding at the tnp translation initiation site is demonstrated in vitro. Two mutations that alter tnp translation in vivo are shown to have corresponding effects in vitro. Most importantly, RNA-OUT/RNA-IN pairing is shown to block ribosome binding. In conjunction with the work described in the accompanying paper, we propose that inhibition of ribosome binding also occurs in vivo, and that it is sufficient to account for control. Implications for translational control in analogous systems are discussed. Previous ArticleNext Article Volume 9Issue 41 April 1990In this issue RelatedDetailsLoading ..." @default.
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• W150762014 date "1990-04-01" @default.
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• W150762014 title "The IS10 antisense RNA blocks ribosome binding at the transposase translation initiation site." @default.
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• W150762014 doi "https://doi.org/10.1002/j.1460-2075.1990.tb08235.x" @default.
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