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• W1507962775 endingPage "945" @default.
• W1507962775 startingPage "934" @default.
• W1507962775 abstract "Summary F‐box proteins function in the recruitment of proteins for SCF ubiquitination and proteasome degradation. Here, we studied the role of F bp1, a nonessential F ‐box protein of the tomato pathogen F usarium oxysporum f. sp. lycopersici . The Δ fbp1 mutant showed a significant delay in the production of wilt symptoms on tomato plants and was impaired in invasive growth on cellophane membranes and on living plant tissue. To search for target proteins recruited by F bp1, a combination of sodium dodecylsulphate‐polyacrylamide gel electrophoresis ( SDS‐PAGE ) and matrix‐assisted laser desorption/ionization time‐of‐flight/time‐of‐flight ( MALDI‐TOF / TOF ) was used to compare proteins in mycelia of the wild‐type and Δ fbp1 mutant. The proteomic approach identified 41 proteins differing significantly in abundance between the two strains, 17 of which were more abundant in the Δ fbp1 mutant, suggesting a possible regulation by proteasome degradation. Interestingly, several of the identified proteins were related to vesicle trafficking. Microscopic analysis revealed an impairment of the Δ fbp1 strain in directional growth and in the structure of the S pitzenkörper, suggesting a role of F bp1 in hyphal orientation. Our results indicate that F bp1 regulates protein turnover and pathogenicity in F . oxysporum ." @default.
• W1507962775 created "2016-06-24" @default.
• W1507962775 creator A5059260521 @default.
• W1507962775 creator A5076837145 @default.
• W1507962775 date "2013-07-16" @default.
• W1507962775 modified "2023-10-12" @default.
• W1507962775 title "Proteomic identification of potential target proteins regulated by the SCF^F^bp1-mediated proteolysis pathway in<i>Fusarium oxysporum</i>" @default.
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• W1507962775 doi "https://doi.org/10.1111/mpp.12060" @default.
• W1507962775 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6638928" @default.
• W1507962775 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23855991" @default.
• W1507962775 hasPublicationYear "2013" @default.
• W1507962775 type Work @default.

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