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• W1508253362 abstract "A significant characteristic of the herpesviruses is that they form latent infections in infected hosts, and can be reactivated to again induce lytic infections by stressors. This thesis deals with an epidemiological investigation of equine herpesvirus infection, particularly gammaherpesvirus infections, in foals and if there was evidence of reactivation of latent virus infections by stressors such as those associated with weaning.A longitudinal study of EHV infections in young foals and the effect of stressors such as weaning on the prevalence of virus infection was undertaken by the detection of DNA and mRNA of EHV2, EHV5, EHV1 and EHV4 in peripheral blood leukocyte (PBL) and nasal swabs (NS) from 13 mares and 46 foals in 4 stables. EHV2 and EHV5 infections were detected commonly in the study population but infections with the alphaherpesviruses EHV1 and EHV4 were not detected, although lytic infections by the alphaherpesviruses may have been missed due to the frequency of sampling. Age differences in the prevalence of EHV2 and EHV5 infection were detected: the prevalence of EHV2 was higher in young foals than in older foals and adult animals; the prevalence of EHV5 was higher in older foals and adults than in younger foals. The prevalence of EHV2 and EHV5 infection increased in association with weaning, presumably in association with stressors associated with weaning, but was not clearly associated with disease in the weaned animals. It was also observed that EHV5 produced a transient lytic infection in PBL of young foals but tended to produce a persistent lytic infection of PBL in older foals and adults. Persistent lytic EHV5 infections of iÝ37 weeks duration were also detected in 2 of 13 adult mares and this has not been reported previously. The persistent lytic infection of PBL was not associated with the detection of virus in NS and the mares with persistent lytic infection of PBL with EHV5 did not transfer the infection to their foals. To determine if any of the animals examined were latently infected with the alphaherpesviruses, an examination for transcripts of genes 63 and 64 of EHV1 and EHV4, putative latency-associated transcripts (LAT) of EHV, was undertaken. Evidence of these transcripts was detected in PBL and bronchiolar lymph nodes in the absence of transcripts of the structural gB, supporting previous studies indicating that transcripts of genes 63 and 64 may represent LAT. In PBL, EHV1 gene 64 RNA transcripts but not gene 63 transcripts were detected in PBL. In bronchiolar lymph nodes, EHV1 gene 64 (but not gene 63) RNA transcripts were detected. In contrast, EHV4 infection was detected in the trigeminal ganglia only and there was no evidence of EHV4 infection in lymph nodes or PBL. In the trigeminal ganglion, EHV4 gB DNA and gene 63 RNA transcripts were detected. The presence of RNA transcripts of EHV1 gene 64 in PBL and lymph node in the absence of any evidence of the replication of structural proteins suggests PBL and lymph node are sites of EHV1 latent infections. The presence of EHV4 gene 63 transcripts in trigeminal ganglia in the absence of any evidence of replication of structural proteins suggests the trigeminal ganglion is the major site of latency of EHV4.As a potential means of detecting latency of the gammaherpesvirus EHV2, 4 EHV2 genes ORF74, E4, E8 and E10 were selected as having possible roles during EHV2 latency based on sequence analysis and comparison with gene products identified or postulated as having roles in latency in other gammaherpesviruses. Kinetic transcription of these genes was evaluated in an in vitro time course study using a non neuronal cell line (equine kidney [EK] cells). While the gB and gH genes encoding structural glycoproteins were abundantly transcribed in vitro, the 4 putative EHV2 latency-associated genes were minimally transcribed during lytic infection in EK cells, a result analogous to results obtained for the expression of LATs in other gammaherpesviruses. Attempts to demonstrate transcription products of these genes in PBL or other tissues of horsed presumed to be latently infected with EHV2 (in which gB transcripts had been detected previously) and actively infected with EHV2 (in which gB transcripts had been detected at the time of sampling), were unsuccessful." @default.
• W1508253362 created "2016-06-24" @default.
• W1508253362 creator A5089957694 @default.
• W1508253362 date "2008-01-01" @default.
• W1508253362 modified "2023-10-02" @default.
• W1508253362 title "Latent equine herpesvirus infections in horses" @default.
• W1508253362 cites W101586874 @default.
• W1508253362 cites W106021668 @default.
• W1508253362 cites W1207182455 @default.
• W1508253362 cites W1223737510 @default.
• W1508253362 cites W131938856 @default.
• W1508253362 cites W1480646191 @default.
• W1508253362 cites W1481110604 @default.
• W1508253362 cites W1483350500 @default.
• W1508253362 cites W1486330483 @default.
• W1508253362 cites W1489925180 @default.
• W1508253362 cites W1490404888 @default.
• W1508253362 cites W1502566225 @default.
• W1508253362 cites W1503193187 @default.
• W1508253362 cites W1504584349 @default.
• W1508253362 cites W1504653733 @default.
• W1508253362 cites W1509673417 @default.
• W1508253362 cites W1510529168 @default.
• W1508253362 cites W1515591091 @default.
• W1508253362 cites W1518348613 @default.
• W1508253362 cites W1520578644 @default.
• W1508253362 cites W1530629109 @default.
• W1508253362 cites W1532938692 @default.
• W1508253362 cites W1542413094 @default.
• W1508253362 cites W1547375868 @default.
• W1508253362 cites W1550806778 @default.
• W1508253362 cites W1555757601 @default.
• W1508253362 cites W1557680815 @default.
• W1508253362 cites W1563487810 @default.
• W1508253362 cites W1566845420 @default.
• W1508253362 cites W1567756652 @default.
• W1508253362 cites W156896671 @default.
• W1508253362 cites W1569131314 @default.
• W1508253362 cites W1573116795 @default.
• W1508253362 cites W1573146315 @default.
• W1508253362 cites W1577579846 @default.
• W1508253362 cites W1577671916 @default.
• W1508253362 cites W1578545176 @default.
• W1508253362 cites W1578735362 @default.
• W1508253362 cites W1586413672 @default.
• W1508253362 cites W1587318259 @default.
• W1508253362 cites W1588246673 @default.
• W1508253362 cites W1588317967 @default.
• W1508253362 cites W1588860880 @default.
• W1508253362 cites W1592653658 @default.
• W1508253362 cites W1593240425 @default.
• W1508253362 cites W1597998272 @default.
• W1508253362 cites W1605241030 @default.
• W1508253362 cites W160997050 @default.
• W1508253362 cites W1652751844 @default.
• W1508253362 cites W1673901587 @default.
• W1508253362 cites W1688924817 @default.
• W1508253362 cites W1709749582 @default.
• W1508253362 cites W172481923 @default.
• W1508253362 cites W1738338042 @default.
• W1508253362 cites W1747817694 @default.
• W1508253362 cites W178341688 @default.
• W1508253362 cites W1783529936 @default.
• W1508253362 cites W1797937433 @default.
• W1508253362 cites W1802351933 @default.
• W1508253362 cites W1805432575 @default.
• W1508253362 cites W1808773443 @default.
• W1508253362 cites W1817074176 @default.
• W1508253362 cites W1850750660 @default.
• W1508253362 cites W1855344496 @default.
• W1508253362 cites W1855952875 @default.
• W1508253362 cites W1866639252 @default.
• W1508253362 cites W1877231298 @default.
• W1508253362 cites W1881486655 @default.
• W1508253362 cites W1889946000 @default.
• W1508253362 cites W1891937459 @default.
• W1508253362 cites W1910404793 @default.
• W1508253362 cites W1911239883 @default.
• W1508253362 cites W1915622567 @default.
• W1508253362 cites W1919926061 @default.
• W1508253362 cites W1925223636 @default.
• W1508253362 cites W1934473991 @default.
• W1508253362 cites W1945061030 @default.
• W1508253362 cites W1948099035 @default.
• W1508253362 cites W1950367760 @default.
• W1508253362 cites W1950773349 @default.
• W1508253362 cites W1954109446 @default.
• W1508253362 cites W1956882355 @default.
• W1508253362 cites W1958548619 @default.
• W1508253362 cites W1958619266 @default.
• W1508253362 cites W1961266051 @default.
• W1508253362 cites W1962153893 @default.
• W1508253362 cites W1963783604 @default.
• W1508253362 cites W1963953894 @default.
• W1508253362 cites W1964935018 @default.
• W1508253362 cites W1965217295 @default.
• W1508253362 cites W1966617602 @default.
• W1508253362 cites W1967551843 @default.
• W1508253362 cites W1967597957 @default.
• W1508253362 cites W1968328677 @default.

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