Matches in SemOpenAlex for { <https://semopenalex.org/work/W1508365798> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W1508365798 endingPage "20708" @default.
- W1508365798 startingPage "20705" @default.
- W1508365798 abstract "The D-glucuronosyl- (GlcA) and N-acetyl-D-glucosaminyl- (GlcNAc) transferase reactions involved in heparin/heparan sulfate biosynthesis were assayed, measuring transfer of radiolabeled GlcA or GlcNAc monosaccharide units from the corresponding UDP-sugars to the appropriate oligosaccharide acceptors. The assays were applied to enzyme purification from bovine serum. The two activities remained inseparable through a series of different chromatographic steps, resulting in approximately -2000-fold purification. Further purification was achieved by chromatofocusing, which showed an isoelectric point of pH approximately -7.0, similar for both activities. SDS-polyacrylamide gel electrophoresis (PAGE) of subfractions from the chromatofocusing procedure revealed an approximately 70-kDa protein in amounts reflecting enzyme activity. SDS-PAGE followed by extraction of gel segments and renaturation of proteins showed that the GlcA- and GlcNAc-transferase activities were both recovered from the same single segment, corresponding to the 70-kDa component. It is proposed that the two glycosyltransferase reactions are catalyzed by the same Golgi enzyme (see also Lidholt, K., Weinke, J. L., Kiser, C. S., Lugemwa, F. N., Bame, K. J., Cheifetz, S., Massague, J., Lindahl, U., and Esko, J. D. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 2267-2271)." @default.
- W1508365798 created "2016-06-24" @default.
- W1508365798 creator A5003537785 @default.
- W1508365798 creator A5039920795 @default.
- W1508365798 creator A5088174305 @default.
- W1508365798 date "1993-10-01" @default.
- W1508365798 modified "2023-10-13" @default.
- W1508365798 title "Biosynthesis of heparin/heparan sulfate. Identification of a 70-kDa protein catalyzing both the D-glucuronosyl- and the N-acetyl-D-glucosaminyltransferase reactions." @default.
- W1508365798 cites W1500163587 @default.
- W1508365798 cites W1542515897 @default.
- W1508365798 cites W1557228498 @default.
- W1508365798 cites W1938067578 @default.
- W1508365798 cites W1989220875 @default.
- W1508365798 cites W1996435499 @default.
- W1508365798 cites W2041620275 @default.
- W1508365798 cites W2239405256 @default.
- W1508365798 cites W2411333327 @default.
- W1508365798 cites W293000020 @default.
- W1508365798 cites W3173300771 @default.
- W1508365798 cites W323023611 @default.
- W1508365798 cites W40028505 @default.
- W1508365798 cites W4251144546 @default.
- W1508365798 cites W4376596386 @default.
- W1508365798 doi "https://doi.org/10.1016/s0021-9258(19)36835-8" @default.
- W1508365798 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8407890" @default.
- W1508365798 hasPublicationYear "1993" @default.
- W1508365798 type Work @default.
- W1508365798 sameAs 1508365798 @default.
- W1508365798 citedByCount "83" @default.
- W1508365798 countsByYear W15083657982014 @default.
- W1508365798 countsByYear W15083657982021 @default.
- W1508365798 countsByYear W15083657982022 @default.
- W1508365798 crossrefType "journal-article" @default.
- W1508365798 hasAuthorship W1508365798A5003537785 @default.
- W1508365798 hasAuthorship W1508365798A5039920795 @default.
- W1508365798 hasAuthorship W1508365798A5088174305 @default.
- W1508365798 hasBestOaLocation W15083657981 @default.
- W1508365798 hasConcept C104317684 @default.
- W1508365798 hasConcept C178790620 @default.
- W1508365798 hasConcept C185592680 @default.
- W1508365798 hasConcept C2777557582 @default.
- W1508365798 hasConcept C2778041096 @default.
- W1508365798 hasConcept C2778343803 @default.
- W1508365798 hasConcept C553450214 @default.
- W1508365798 hasConcept C55493867 @default.
- W1508365798 hasConcept C71240020 @default.
- W1508365798 hasConceptScore W1508365798C104317684 @default.
- W1508365798 hasConceptScore W1508365798C178790620 @default.
- W1508365798 hasConceptScore W1508365798C185592680 @default.
- W1508365798 hasConceptScore W1508365798C2777557582 @default.
- W1508365798 hasConceptScore W1508365798C2778041096 @default.
- W1508365798 hasConceptScore W1508365798C2778343803 @default.
- W1508365798 hasConceptScore W1508365798C553450214 @default.
- W1508365798 hasConceptScore W1508365798C55493867 @default.
- W1508365798 hasConceptScore W1508365798C71240020 @default.
- W1508365798 hasIssue "28" @default.
- W1508365798 hasLocation W15083657981 @default.
- W1508365798 hasOpenAccess W1508365798 @default.
- W1508365798 hasPrimaryLocation W15083657981 @default.
- W1508365798 hasRelatedWork W2026251112 @default.
- W1508365798 hasRelatedWork W2040572458 @default.
- W1508365798 hasRelatedWork W2266671018 @default.
- W1508365798 hasRelatedWork W2366226386 @default.
- W1508365798 hasRelatedWork W2606892147 @default.
- W1508365798 hasRelatedWork W2937682530 @default.
- W1508365798 hasRelatedWork W2949862580 @default.
- W1508365798 hasRelatedWork W3016164001 @default.
- W1508365798 hasRelatedWork W4235239922 @default.
- W1508365798 hasRelatedWork W656365412 @default.
- W1508365798 hasVolume "268" @default.
- W1508365798 isParatext "false" @default.
- W1508365798 isRetracted "false" @default.
- W1508365798 magId "1508365798" @default.
- W1508365798 workType "article" @default.