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• W1508439085 abstract "9 SAMMANFATTNING 10 LIST OF SCIENTIFIC PAPERS 11 LIST OF ABBREVIATIONS 14 AIMS OF THIS STUDY 16 INTRODUCTION 17 1.1 Epidemiology of cardiovascular disease 17 1.2 Atherosclerosis 18 1.3 Surgical treatment of cardiovascular disease 18 1.4 The use of vein grafts in cardiovascular disease 19 1.5 Intimal hyperplasia 20 1.6 The development of vein graft disease 20 1.7 The impact of hemodynamics 22 1.8 Leukocyte recruitment 23 1.9 Platelets 26 1.10 Selectins 27 1.11 Integrins 29 METHODS AND MATERIALS 31 2.1 Animal model 31 2.2 Mouse strain 33 2.3 Antibodies 34 2.4 Antibody purification 34 2.5 Antibody injection 34 2.6 Blood sampling and whole blood cell counting 36 2.7 Whole blood aggregation function test 36 2.8 Intravital microscopy 36 2.9 Harvesting of vein grafts 37 2.10 Scanning electron microscopy (SEM) 37 2.11 Confocal microscopy 37 2.12 Immunohistochemistry (IHC) 38 2.13 Statistical analysis 38 RESULTS AND DISCUSSION 39 3.1 Denudation and regeneration of endothelium after vascular grafting in WT mice. 39 3.1.1 Denudation of endothelium in vascular grafts. 39 3.1.2 Regeneration of endothelium in VGs. 40 3.2 Selectins mediate inflammation and IH development in VGs. 40 3.2.1 Deficiency of selectins attenuates leukocyte recruitment on endothelium and IH development in VGs. 40 3.2.2 Early blockage of the P-selectin/PSGL-1 adhesion pathway reduces intimal leukocyte invasion and IH in VGs. 41 3.3 Platelets mediate VG failure. 42 3.3.1 Early platelet depletion reduces IH development 42" @default.
• W1508439085 created "2016-06-24" @default.
• W1508439085 creator A5090358128 @default.
• W1508439085 date "2015-03-20" @default.
• W1508439085 modified "2023-09-29" @default.
• W1508439085 title "Interactions between leukocytes, platelets and the endothelium in vein graft failure" @default.
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