Matches in SemOpenAlex for { <https://semopenalex.org/work/W1508548249> ?p ?o ?g. }
Showing items 1 to 66 of
66
with 100 items per page.
- W1508548249 endingPage "1270" @default.
- W1508548249 startingPage "1264" @default.
- W1508548249 abstract "Abstract Two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) was used to examine and compare the products of the Qa-1 locus. Analysis of Qa-1 isolated from detergent lysates of surface labeled cells indicated this molecule was a slightly acidic 48,000 to 50,000 dalton glycoprotein that displayed little charge heterogeneity on resting lymphocytes. The level of expression and degree of charge heterogeneity were both increased on activated lymphocytes. Direct comparison of the Qa-1b, Qa-1c, and Qa-1d allelic products by 2-D PAGE revealed that these three molecules could be distinguished from one another on the basis of isoelectric point, indicating that they were distinct at the molecular level. Comparison of Qa-1 isolated from several Qa-1b strains did not detect additional polymorphism. Removal of asparagine-linked oligosaccharides by treatment with endoglycosidase F indicated that carbohydrate contributed 10,000 to 12,000 to the m.w. of these allelic products. Comparative 2-D PAGE analysis could not distinguish between the deglycosylated Qa-1b, Qa-1c, and Qa-1d allelic products, implying that these molecules have similar primary structures. Peptide mapping supported this conclusion. Proteolytic digestion of the deglycosylated Qa-1b and Qa-1c allelic products resulted in identical peptide map patterns; such treatment of the deglycosylated Qa-1d allelic product produced a slightly different pattern. Peptide mapping analysis also demonstrated that the Tlaa and Qa-1a allelic products were distinct from one another, as well as being very different from the other three Qa-1 allelic products." @default.
- W1508548249 created "2016-06-24" @default.
- W1508548249 creator A5028471043 @default.
- W1508548249 creator A5064944404 @default.
- W1508548249 creator A5081952589 @default.
- W1508548249 date "1985-08-01" @default.
- W1508548249 modified "2023-09-24" @default.
- W1508548249 title "The Qa-1 alloantigens. III. Biochemical analysis of the structure and extent of polymorphism of the Qa-1 allelic products." @default.
- W1508548249 doi "https://doi.org/10.4049/jimmunol.135.2.1264" @default.
- W1508548249 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3925004" @default.
- W1508548249 hasPublicationYear "1985" @default.
- W1508548249 type Work @default.
- W1508548249 sameAs 1508548249 @default.
- W1508548249 citedByCount "10" @default.
- W1508548249 countsByYear W15085482492017 @default.
- W1508548249 crossrefType "journal-article" @default.
- W1508548249 hasAuthorship W1508548249A5028471043 @default.
- W1508548249 hasAuthorship W1508548249A5064944404 @default.
- W1508548249 hasAuthorship W1508548249A5081952589 @default.
- W1508548249 hasBestOaLocation W15085482491 @default.
- W1508548249 hasConcept C101660505 @default.
- W1508548249 hasConcept C104317684 @default.
- W1508548249 hasConcept C108625454 @default.
- W1508548249 hasConcept C153911025 @default.
- W1508548249 hasConcept C180754005 @default.
- W1508548249 hasConcept C181199279 @default.
- W1508548249 hasConcept C185592680 @default.
- W1508548249 hasConcept C2779281246 @default.
- W1508548249 hasConcept C50476208 @default.
- W1508548249 hasConcept C55493867 @default.
- W1508548249 hasConcept C84597430 @default.
- W1508548249 hasConcept C86803240 @default.
- W1508548249 hasConceptScore W1508548249C101660505 @default.
- W1508548249 hasConceptScore W1508548249C104317684 @default.
- W1508548249 hasConceptScore W1508548249C108625454 @default.
- W1508548249 hasConceptScore W1508548249C153911025 @default.
- W1508548249 hasConceptScore W1508548249C180754005 @default.
- W1508548249 hasConceptScore W1508548249C181199279 @default.
- W1508548249 hasConceptScore W1508548249C185592680 @default.
- W1508548249 hasConceptScore W1508548249C2779281246 @default.
- W1508548249 hasConceptScore W1508548249C50476208 @default.
- W1508548249 hasConceptScore W1508548249C55493867 @default.
- W1508548249 hasConceptScore W1508548249C84597430 @default.
- W1508548249 hasConceptScore W1508548249C86803240 @default.
- W1508548249 hasIssue "2" @default.
- W1508548249 hasLocation W15085482491 @default.
- W1508548249 hasLocation W15085482492 @default.
- W1508548249 hasOpenAccess W1508548249 @default.
- W1508548249 hasPrimaryLocation W15085482491 @default.
- W1508548249 hasRelatedWork W1862377560 @default.
- W1508548249 hasRelatedWork W2000479887 @default.
- W1508548249 hasRelatedWork W2022755580 @default.
- W1508548249 hasRelatedWork W2033276838 @default.
- W1508548249 hasRelatedWork W2057739827 @default.
- W1508548249 hasRelatedWork W2107205263 @default.
- W1508548249 hasRelatedWork W2200792017 @default.
- W1508548249 hasRelatedWork W4255226035 @default.
- W1508548249 hasRelatedWork W96924620 @default.
- W1508548249 hasRelatedWork W1993930956 @default.
- W1508548249 hasVolume "135" @default.
- W1508548249 isParatext "false" @default.
- W1508548249 isRetracted "false" @default.
- W1508548249 magId "1508548249" @default.
- W1508548249 workType "article" @default.