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- W1508587432 abstract "Fifteen related ligation-independent cloning vectors were constructed for high-throughput cloning and purification of proteins. The vectors encode a TEV protease site for removal of tags that facilitate pro tein purification (his-tag) or improve solubility (MBP, GST). Specialized vectors allow coexpression and copurification of interacting proteins, or in vivo removal of MBP by TVMV protease to improve screening and purification. All target genes and vectors are processed by the same protocols, which we describe here." @default.
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- W1508587432 date "2009-01-01" @default.
- W1508587432 modified "2023-10-04" @default.
- W1508587432 title "A Family of LIC Vectors for High-Throughput Cloning and Purification of Proteins" @default.
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- W1508587432 doi "https://doi.org/10.1007/978-1-59745-196-3_7" @default.
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