Matches in SemOpenAlex for { <https://semopenalex.org/work/W1508786321> ?p ?o ?g. }
- W1508786321 endingPage "6900" @default.
- W1508786321 startingPage "6894" @default.
- W1508786321 abstract "Antioxidant response elements (AREs) containing 12-O-tetradecanoylphorbol-13-acetate response element (TRE) (perfect AP1) and TRE-like (imperfect AP1) elements mediate high basal transcription of the NAD(P)H:quinone oxidoreductase1 (NQO1) and glutathione S-transferase Ya genes in tumor cells and its induction in response to xenobiotics and antioxidants. Mutations in the human NQO1 gene ARE (hARE) revealed the requirement for two TRE or TRE-like elements arranged in inverse orientation at the interval of three base pairs and a GC box for optimal expression and β-naphthoflavone induction of the NQO1 gene. A single TRE element from the human collagenase gene failed to respond to β-naphthoflavone. These results demonstrate that ARE (2 × TRE or TRE-like elements)-containing detoxifying enzyme genes and not genes that contain 1 × TRE are responsive to xenobiotics and antioxidants. Bandshift assays showed shifting of a complex of more or less similar mobility with hARE and TRE that could be competed by each other. Mutations in the 3′-TRE of the NQO1 gene hARE eliminated binding of nuclear proteins to the hARE and resulted in the loss of basal and induced expression, indicating that 3′-TRE is the most important element within the hARE. 5′-TRE-like element within the NQO1 gene hARE is required for xenobiotic response but may not bind to the nuclear proteins by itself. The GC box located immmediately following the 3′-TRE is required for optimal expression and induction of the NQO1 gene. The comparison of AREs from several different genes indicated the requirement for specific arrangement and spacing of two TRE and TRE-like elements within the AREs. Antioxidant response elements (AREs) containing 12-O-tetradecanoylphorbol-13-acetate response element (TRE) (perfect AP1) and TRE-like (imperfect AP1) elements mediate high basal transcription of the NAD(P)H:quinone oxidoreductase1 (NQO1) and glutathione S-transferase Ya genes in tumor cells and its induction in response to xenobiotics and antioxidants. Mutations in the human NQO1 gene ARE (hARE) revealed the requirement for two TRE or TRE-like elements arranged in inverse orientation at the interval of three base pairs and a GC box for optimal expression and β-naphthoflavone induction of the NQO1 gene. A single TRE element from the human collagenase gene failed to respond to β-naphthoflavone. These results demonstrate that ARE (2 × TRE or TRE-like elements)-containing detoxifying enzyme genes and not genes that contain 1 × TRE are responsive to xenobiotics and antioxidants. Bandshift assays showed shifting of a complex of more or less similar mobility with hARE and TRE that could be competed by each other. Mutations in the 3′-TRE of the NQO1 gene hARE eliminated binding of nuclear proteins to the hARE and resulted in the loss of basal and induced expression, indicating that 3′-TRE is the most important element within the hARE. 5′-TRE-like element within the NQO1 gene hARE is required for xenobiotic response but may not bind to the nuclear proteins by itself. The GC box located immmediately following the 3′-TRE is required for optimal expression and induction of the NQO1 gene. The comparison of AREs from several different genes indicated the requirement for specific arrangement and spacing of two TRE and TRE-like elements within the AREs." @default.
- W1508786321 created "2016-06-24" @default.
- W1508786321 creator A5027903995 @default.
- W1508786321 creator A5031752665 @default.
- W1508786321 creator A5069272791 @default.
- W1508786321 creator A5073371884 @default.
- W1508786321 date "1995-03-01" @default.
- W1508786321 modified "2023-09-29" @default.
- W1508786321 title "ARE- and TRE-mediated Regulation of Gene Expression" @default.
- W1508786321 cites W1483046275 @default.
- W1508786321 cites W1486158855 @default.
- W1508786321 cites W1516457342 @default.
- W1508786321 cites W1561096292 @default.
- W1508786321 cites W1581676497 @default.
- W1508786321 cites W1603378617 @default.
- W1508786321 cites W1604748982 @default.
- W1508786321 cites W1652134433 @default.
- W1508786321 cites W1858268805 @default.
- W1508786321 cites W1967544359 @default.
- W1508786321 cites W1967692811 @default.
- W1508786321 cites W1972626671 @default.
- W1508786321 cites W1973595140 @default.
- W1508786321 cites W1975987535 @default.
- W1508786321 cites W1993243592 @default.
- W1508786321 cites W2020178430 @default.
- W1508786321 cites W2023666553 @default.
- W1508786321 cites W2037753946 @default.
- W1508786321 cites W2045965817 @default.
- W1508786321 cites W2064035407 @default.
- W1508786321 cites W2068577315 @default.
- W1508786321 cites W2073307134 @default.
- W1508786321 cites W2078471960 @default.
- W1508786321 cites W2083287953 @default.
- W1508786321 cites W2092449641 @default.
- W1508786321 cites W2107359522 @default.
- W1508786321 cites W2113208052 @default.
- W1508786321 cites W2153247725 @default.
- W1508786321 cites W2174669178 @default.
- W1508786321 cites W4302775936 @default.
- W1508786321 cites W77902439 @default.
- W1508786321 doi "https://doi.org/10.1074/jbc.270.12.6894" @default.
- W1508786321 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7896838" @default.
- W1508786321 hasPublicationYear "1995" @default.
- W1508786321 type Work @default.
- W1508786321 sameAs 1508786321 @default.
- W1508786321 citedByCount "123" @default.
- W1508786321 countsByYear W15087863212012 @default.
- W1508786321 countsByYear W15087863212013 @default.
- W1508786321 countsByYear W15087863212014 @default.
- W1508786321 countsByYear W15087863212015 @default.
- W1508786321 countsByYear W15087863212016 @default.
- W1508786321 countsByYear W15087863212017 @default.
- W1508786321 countsByYear W15087863212018 @default.
- W1508786321 countsByYear W15087863212019 @default.
- W1508786321 countsByYear W15087863212020 @default.
- W1508786321 countsByYear W15087863212022 @default.
- W1508786321 crossrefType "journal-article" @default.
- W1508786321 hasAuthorship W1508786321A5027903995 @default.
- W1508786321 hasAuthorship W1508786321A5031752665 @default.
- W1508786321 hasAuthorship W1508786321A5069272791 @default.
- W1508786321 hasAuthorship W1508786321A5073371884 @default.
- W1508786321 hasBestOaLocation W15087863211 @default.
- W1508786321 hasConcept C101762097 @default.
- W1508786321 hasConcept C102280478 @default.
- W1508786321 hasConcept C104317684 @default.
- W1508786321 hasConcept C132376346 @default.
- W1508786321 hasConcept C138885662 @default.
- W1508786321 hasConcept C150194340 @default.
- W1508786321 hasConcept C153911025 @default.
- W1508786321 hasConcept C165864922 @default.
- W1508786321 hasConcept C179716448 @default.
- W1508786321 hasConcept C179926584 @default.
- W1508786321 hasConcept C41895202 @default.
- W1508786321 hasConcept C54355233 @default.
- W1508786321 hasConcept C86339819 @default.
- W1508786321 hasConcept C86803240 @default.
- W1508786321 hasConcept C95444343 @default.
- W1508786321 hasConceptScore W1508786321C101762097 @default.
- W1508786321 hasConceptScore W1508786321C102280478 @default.
- W1508786321 hasConceptScore W1508786321C104317684 @default.
- W1508786321 hasConceptScore W1508786321C132376346 @default.
- W1508786321 hasConceptScore W1508786321C138885662 @default.
- W1508786321 hasConceptScore W1508786321C150194340 @default.
- W1508786321 hasConceptScore W1508786321C153911025 @default.
- W1508786321 hasConceptScore W1508786321C165864922 @default.
- W1508786321 hasConceptScore W1508786321C179716448 @default.
- W1508786321 hasConceptScore W1508786321C179926584 @default.
- W1508786321 hasConceptScore W1508786321C41895202 @default.
- W1508786321 hasConceptScore W1508786321C54355233 @default.
- W1508786321 hasConceptScore W1508786321C86339819 @default.
- W1508786321 hasConceptScore W1508786321C86803240 @default.
- W1508786321 hasConceptScore W1508786321C95444343 @default.
- W1508786321 hasIssue "12" @default.
- W1508786321 hasLocation W15087863211 @default.
- W1508786321 hasOpenAccess W1508786321 @default.
- W1508786321 hasPrimaryLocation W15087863211 @default.
- W1508786321 hasRelatedWork W1818003313 @default.
- W1508786321 hasRelatedWork W188209930 @default.