Matches in SemOpenAlex for { <https://semopenalex.org/work/W1508889369> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W1508889369 endingPage "334" @default.
- W1508889369 startingPage "329" @default.
- W1508889369 abstract "Abstract Phenylethanolamine N-methyltransferase (EC 2.1.1) was isolated and purified approximately 60-fold from bovine adrenal medullae. The final product appears homogeneous by column chromatography, electrophoresis, and ultracentrifugation. The enzyme gave a s20,w of 3.0 S with a molecular weight by Sephadex chromatography of 38,000. No cofactors were needed for the reaction. The pH optimum was 7.9 in either Tris or borate buffers. Titration with p-hydroxymercuribenzoate showed that 8.5 moles of sulfhydryl were titrated per mole of enzyme of 38,000 molecular weight and that activity was lost on titration of 2 moles of sulfhydryl groups per mole of enzyme. Kinetic measurements indicated a complex reaction mechanism which is best described as a random binding of substrates with S-adenosylmethionine being kinetically preferred as the first substrate bound." @default.
- W1508889369 created "2016-06-24" @default.
- W1508889369 creator A5005343773 @default.
- W1508889369 creator A5036953018 @default.
- W1508889369 date "1970-01-01" @default.
- W1508889369 modified "2023-10-03" @default.
- W1508889369 title "Purification and Properties of Bovine Phenylethanolamine N-Methyltransferase" @default.
- W1508889369 cites W112855116 @default.
- W1508889369 cites W1611482599 @default.
- W1508889369 cites W1620099009 @default.
- W1508889369 cites W1986170134 @default.
- W1508889369 cites W1989054182 @default.
- W1508889369 cites W2003408945 @default.
- W1508889369 cites W2010642998 @default.
- W1508889369 cites W2015199901 @default.
- W1508889369 cites W2034554255 @default.
- W1508889369 cites W2043243758 @default.
- W1508889369 cites W2046906442 @default.
- W1508889369 cites W2053860931 @default.
- W1508889369 cites W2066150587 @default.
- W1508889369 cites W2083945806 @default.
- W1508889369 cites W2087078022 @default.
- W1508889369 cites W2132068224 @default.
- W1508889369 cites W2333363395 @default.
- W1508889369 cites W2340661992 @default.
- W1508889369 doi "https://doi.org/10.1016/s0021-9258(18)63396-4" @default.
- W1508889369 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/5412063" @default.
- W1508889369 hasPublicationYear "1970" @default.
- W1508889369 type Work @default.
- W1508889369 sameAs 1508889369 @default.
- W1508889369 citedByCount "107" @default.
- W1508889369 countsByYear W15088893692012 @default.
- W1508889369 countsByYear W15088893692013 @default.
- W1508889369 countsByYear W15088893692015 @default.
- W1508889369 countsByYear W15088893692020 @default.
- W1508889369 crossrefType "journal-article" @default.
- W1508889369 hasAuthorship W1508889369A5005343773 @default.
- W1508889369 hasAuthorship W1508889369A5036953018 @default.
- W1508889369 hasBestOaLocation W15088893691 @default.
- W1508889369 hasConcept C159167319 @default.
- W1508889369 hasConcept C181199279 @default.
- W1508889369 hasConcept C185592680 @default.
- W1508889369 hasConcept C2776804559 @default.
- W1508889369 hasConcept C2779546955 @default.
- W1508889369 hasConcept C33288867 @default.
- W1508889369 hasConcept C552990157 @default.
- W1508889369 hasConcept C55493867 @default.
- W1508889369 hasConcept C91965660 @default.
- W1508889369 hasConceptScore W1508889369C159167319 @default.
- W1508889369 hasConceptScore W1508889369C181199279 @default.
- W1508889369 hasConceptScore W1508889369C185592680 @default.
- W1508889369 hasConceptScore W1508889369C2776804559 @default.
- W1508889369 hasConceptScore W1508889369C2779546955 @default.
- W1508889369 hasConceptScore W1508889369C33288867 @default.
- W1508889369 hasConceptScore W1508889369C552990157 @default.
- W1508889369 hasConceptScore W1508889369C55493867 @default.
- W1508889369 hasConceptScore W1508889369C91965660 @default.
- W1508889369 hasIssue "2" @default.
- W1508889369 hasLocation W15088893691 @default.
- W1508889369 hasOpenAccess W1508889369 @default.
- W1508889369 hasPrimaryLocation W15088893691 @default.
- W1508889369 hasRelatedWork W188541036 @default.
- W1508889369 hasRelatedWork W1987593606 @default.
- W1508889369 hasRelatedWork W2020685702 @default.
- W1508889369 hasRelatedWork W203029489 @default.
- W1508889369 hasRelatedWork W2041626287 @default.
- W1508889369 hasRelatedWork W2077049250 @default.
- W1508889369 hasRelatedWork W2083740534 @default.
- W1508889369 hasRelatedWork W2342325929 @default.
- W1508889369 hasRelatedWork W2493866067 @default.
- W1508889369 hasRelatedWork W2516701513 @default.
- W1508889369 hasVolume "245" @default.
- W1508889369 isParatext "false" @default.
- W1508889369 isRetracted "false" @default.
- W1508889369 magId "1508889369" @default.
- W1508889369 workType "article" @default.