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- W1508899278 startingPage "1891" @default.
- W1508899278 abstract "The N-succinimidyl esters of various glycine-conjugated bile acid 3-hemisuccinates and 3-hemiglutarates have been prepared for use in immunoassay. Unconjugated bile acids were condensed with p-nitrophenol to form the activated esters. Subsequent reaction with 2, 2, 2-trichloroethyl glycinate provided the glycine-conjugated trichloroethyl esters. On treatment with succinic anhydride and glutaric anhydride in pyridine, the glycine-conjugated bile acids were led to the 3-hemisuccinates and 3-hemiglutarates, respectively. Condensation of these half esters with N-hydroxysuccinimide was effected by the use of water-soluble carbodiimide to provide the N-succinimidyl esters. Elimination of the protecting group in the side chain was achieved by reduction with zinc dust in tetrahydrofuran-potassium dihydrogen phosphate solution, yielding the desired haptens." @default.
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- W1508899278 date "1984-01-01" @default.
- W1508899278 modified "2023-09-27" @default.
- W1508899278 title "Preparation of new haptens for use in immunoassay of glycine-conjugated bile acids." @default.
- W1508899278 doi "https://doi.org/10.1248/cpb.32.1891" @default.
- W1508899278 hasPublicationYear "1984" @default.
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