FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1508962214> ?p ?o ?g. }

• W1508962214 endingPage "11103" @default.
• W1508962214 startingPage "11097" @default.
• W1508962214 abstract "Seven distinct heparin-binding sites have been demonstrated on human apolipoprotein (apo) B-100 by using a combination of digestion with cyanogen bromide or Staphylococcus aureus V-8 protease and heparin-Sepharose affinity chromatography. Based on fragment analysis, the approximate boundaries of the seven binding sites are as follows: site A, residues 5-99; site B, residues 205-279; site C, residues 875-932; site D, residues 2016-2151; site E, residues 3134-3209; site F, 3356-3489; and site G, residues 3659-3719. In sites E and F, two short regions enriched in basic amino acids have been identified, and it is likely that they are responsible for a major portion of the heparin-binding properties of these sites. The relative binding affinity of each of the seven sites was estimated in two ways. First, the affinity was assessed in a ligand blot assay using a 125I-labeled high-reactive heparin subfraction. Second, apoB-100 fragments generated by cyanogen bromide or S. aureus V-8 protease were separated into low- and high-affinity fractions by gradient salt elution of a heparin-Sepharose column. The distribution of the seven binding sites in the two fractions was determined in an immunoblotting assay using antibodies specific to each site, i.e. antibodies raised against synthetic peptide sequences found within each of the seven sites. The results of these two approaches demonstrate that site E and, to a somewhat lesser extent, site F bind to heparin with the highest affinity. Based on the analogy with apoE, in which the high-affinity heparin-binding site coincides with the domain of the protein that interacts with apoB,E (low density lipoprotein) receptors, the results of this study indicate that site E and site F, either singly or in combination, might constitute the receptor binding domain of apoB-100." @default.
• W1508962214 created "2016-06-24" @default.
• W1508962214 creator A5013208324 @default.
• W1508962214 creator A5033190041 @default.
• W1508962214 date "1987-08-01" @default.
• W1508962214 modified "2023-09-28" @default.
• W1508962214 title "Human apolipoprotein B-100 heparin-binding sites." @default.
• W1508962214 cites W14380143 @default.
• W1508962214 cites W1487164341 @default.
• W1508962214 cites W1505230657 @default.
• W1508962214 cites W1507242932 @default.
• W1508962214 cites W1531354789 @default.
• W1508962214 cites W1546262989 @default.
• W1508962214 cites W1548784481 @default.
• W1508962214 cites W1553982335 @default.
• W1508962214 cites W1571261261 @default.
• W1508962214 cites W1736154854 @default.
• W1508962214 cites W1775749144 @default.
• W1508962214 cites W1893651774 @default.
• W1508962214 cites W1920171370 @default.
• W1508962214 cites W1968541049 @default.
• W1508962214 cites W1980092242 @default.
• W1508962214 cites W1991316679 @default.
• W1508962214 cites W1996865203 @default.
• W1508962214 cites W2002892589 @default.
• W1508962214 cites W2006411195 @default.
• W1508962214 cites W2008099488 @default.
• W1508962214 cites W2008104806 @default.
• W1508962214 cites W2012413789 @default.
• W1508962214 cites W2016232050 @default.
• W1508962214 cites W2017640755 @default.
• W1508962214 cites W2021983069 @default.
• W1508962214 cites W2022604481 @default.
• W1508962214 cites W2038097197 @default.
• W1508962214 cites W2049023634 @default.
• W1508962214 cites W2049556830 @default.
• W1508962214 cites W2064421638 @default.
• W1508962214 cites W2082416886 @default.
• W1508962214 cites W2084744450 @default.
• W1508962214 cites W2086426066 @default.
• W1508962214 cites W2092900687 @default.
• W1508962214 cites W2129659164 @default.
• W1508962214 cites W2135503869 @default.
• W1508962214 cites W2136701955 @default.
• W1508962214 cites W2138841151 @default.
• W1508962214 cites W2146023328 @default.
• W1508962214 cites W2146287339 @default.
• W1508962214 cites W2347061855 @default.
• W1508962214 cites W4240237349 @default.
• W1508962214 cites W4248021162 @default.
• W1508962214 doi "https://doi.org/10.1016/s0021-9258(18)60931-7" @default.
• W1508962214 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3301850" @default.
• W1508962214 hasPublicationYear "1987" @default.
• W1508962214 type Work @default.
• W1508962214 sameAs 1508962214 @default.
• W1508962214 citedByCount "184" @default.
• W1508962214 countsByYear W15089622142012 @default.
• W1508962214 countsByYear W15089622142013 @default.
• W1508962214 countsByYear W15089622142014 @default.
• W1508962214 countsByYear W15089622142015 @default.
• W1508962214 countsByYear W15089622142016 @default.
• W1508962214 countsByYear W15089622142017 @default.
• W1508962214 countsByYear W15089622142018 @default.
• W1508962214 countsByYear W15089622142019 @default.
• W1508962214 countsByYear W15089622142020 @default.
• W1508962214 countsByYear W15089622142021 @default.
• W1508962214 countsByYear W15089622142022 @default.
• W1508962214 crossrefType "journal-article" @default.
• W1508962214 hasAuthorship W1508962214A5013208324 @default.
• W1508962214 hasAuthorship W1508962214A5033190041 @default.
• W1508962214 hasBestOaLocation W15089622141 @default.
• W1508962214 hasConcept C107824862 @default.
• W1508962214 hasConcept C185592680 @default.
• W1508962214 hasConcept C2777557582 @default.
• W1508962214 hasConcept C2778163477 @default.
• W1508962214 hasConcept C51639874 @default.
• W1508962214 hasConcept C55493867 @default.
• W1508962214 hasConcept C62746215 @default.
• W1508962214 hasConceptScore W1508962214C107824862 @default.
• W1508962214 hasConceptScore W1508962214C185592680 @default.
• W1508962214 hasConceptScore W1508962214C2777557582 @default.
• W1508962214 hasConceptScore W1508962214C2778163477 @default.
• W1508962214 hasConceptScore W1508962214C51639874 @default.
• W1508962214 hasConceptScore W1508962214C55493867 @default.
• W1508962214 hasConceptScore W1508962214C62746215 @default.
• W1508962214 hasIssue "23" @default.
• W1508962214 hasLocation W15089622141 @default.
• W1508962214 hasOpenAccess W1508962214 @default.
• W1508962214 hasPrimaryLocation W15089622141 @default.
• W1508962214 hasRelatedWork W1528225178 @default.
• W1508962214 hasRelatedWork W1981033203 @default.
• W1508962214 hasRelatedWork W2001620578 @default.
• W1508962214 hasRelatedWork W2012093717 @default.
• W1508962214 hasRelatedWork W2027074537 @default.
• W1508962214 hasRelatedWork W2040295454 @default.
• W1508962214 hasRelatedWork W2071991954 @default.
• W1508962214 hasRelatedWork W2112734882 @default.
• W1508962214 hasRelatedWork W2139741471 @default.

• next