Matches in SemOpenAlex for { <https://semopenalex.org/work/W1509481275> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W1509481275 endingPage "1127" @default.
- W1509481275 startingPage "1122" @default.
- W1509481275 abstract "Human T cell growth factor (TCGF) has been purified more than 800-fold from serum-free lymphocyte conditioned media by utilizing ion exchange chromatography with DEAE-Sepharose, gel filtration with Ultrogel AcA54, and preparative SDS-polyacrylamide gel electrophoresis. This mitogenic protein, which is released by T cells into the incubating media after exposure to a lectin (PHA), has a m.w. of 13,000 as determined by SDS-PAGE and 20,000 to 25,000 on gel filtration and has an isoelectric point of 6.8. The material extracted from acrylamide gels is a single band (or two nearly superimposed bands) when rerun on analytical SDS gels. Partially purified material is unstable even at -70 degrees C and requires the addition of bovine serum albumin or polyethylene glycol to maintain biologic activity. It is sensitive to proteolytic digestion but resistant to nucleases and thiol-reducing agents such as dithiothreitol. Human TCGF can be reversibly denatured with urea or SDS. Material extracted from acrylamide gels has been shown to sustain T lymphoblasts in tissue culture. In contrast to lectins, certain antigens, and crude lymphocyte conditioned media, purified TCGF does not initiate lymphocyte blastogenesis but is a highly selective mitogen for T cells previously activated by exposure to lectins or antigens." @default.
- W1509481275 created "2016-06-24" @default.
- W1509481275 creator A5066250381 @default.
- W1509481275 creator A5083817243 @default.
- W1509481275 date "1982-03-01" @default.
- W1509481275 modified "2023-10-14" @default.
- W1509481275 title "The purification and properties of human T cell growth factor." @default.
- W1509481275 doi "https://doi.org/10.4049/jimmunol.128.3.1122" @default.
- W1509481275 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6976987" @default.
- W1509481275 hasPublicationYear "1982" @default.
- W1509481275 type Work @default.
- W1509481275 sameAs 1509481275 @default.
- W1509481275 citedByCount "52" @default.
- W1509481275 countsByYear W15094812752015 @default.
- W1509481275 crossrefType "journal-article" @default.
- W1509481275 hasAuthorship W1509481275A5066250381 @default.
- W1509481275 hasAuthorship W1509481275A5083817243 @default.
- W1509481275 hasBestOaLocation W15094812751 @default.
- W1509481275 hasConcept C101660505 @default.
- W1509481275 hasConcept C113842279 @default.
- W1509481275 hasConcept C147483822 @default.
- W1509481275 hasConcept C15920480 @default.
- W1509481275 hasConcept C178790620 @default.
- W1509481275 hasConcept C181199279 @default.
- W1509481275 hasConcept C185592680 @default.
- W1509481275 hasConcept C193363816 @default.
- W1509481275 hasConcept C19869320 @default.
- W1509481275 hasConcept C202751555 @default.
- W1509481275 hasConcept C203014093 @default.
- W1509481275 hasConcept C2777824588 @default.
- W1509481275 hasConcept C2779402116 @default.
- W1509481275 hasConcept C2780340462 @default.
- W1509481275 hasConcept C2780365088 @default.
- W1509481275 hasConcept C2780758401 @default.
- W1509481275 hasConcept C38240268 @default.
- W1509481275 hasConcept C43617362 @default.
- W1509481275 hasConcept C50476208 @default.
- W1509481275 hasConcept C521977710 @default.
- W1509481275 hasConcept C54355233 @default.
- W1509481275 hasConcept C55493867 @default.
- W1509481275 hasConcept C81885089 @default.
- W1509481275 hasConcept C86803240 @default.
- W1509481275 hasConcept C86807702 @default.
- W1509481275 hasConcept C96294017 @default.
- W1509481275 hasConceptScore W1509481275C101660505 @default.
- W1509481275 hasConceptScore W1509481275C113842279 @default.
- W1509481275 hasConceptScore W1509481275C147483822 @default.
- W1509481275 hasConceptScore W1509481275C15920480 @default.
- W1509481275 hasConceptScore W1509481275C178790620 @default.
- W1509481275 hasConceptScore W1509481275C181199279 @default.
- W1509481275 hasConceptScore W1509481275C185592680 @default.
- W1509481275 hasConceptScore W1509481275C193363816 @default.
- W1509481275 hasConceptScore W1509481275C19869320 @default.
- W1509481275 hasConceptScore W1509481275C202751555 @default.
- W1509481275 hasConceptScore W1509481275C203014093 @default.
- W1509481275 hasConceptScore W1509481275C2777824588 @default.
- W1509481275 hasConceptScore W1509481275C2779402116 @default.
- W1509481275 hasConceptScore W1509481275C2780340462 @default.
- W1509481275 hasConceptScore W1509481275C2780365088 @default.
- W1509481275 hasConceptScore W1509481275C2780758401 @default.
- W1509481275 hasConceptScore W1509481275C38240268 @default.
- W1509481275 hasConceptScore W1509481275C43617362 @default.
- W1509481275 hasConceptScore W1509481275C50476208 @default.
- W1509481275 hasConceptScore W1509481275C521977710 @default.
- W1509481275 hasConceptScore W1509481275C54355233 @default.
- W1509481275 hasConceptScore W1509481275C55493867 @default.
- W1509481275 hasConceptScore W1509481275C81885089 @default.
- W1509481275 hasConceptScore W1509481275C86803240 @default.
- W1509481275 hasConceptScore W1509481275C86807702 @default.
- W1509481275 hasConceptScore W1509481275C96294017 @default.
- W1509481275 hasIssue "3" @default.
- W1509481275 hasLocation W15094812751 @default.
- W1509481275 hasLocation W15094812752 @default.
- W1509481275 hasOpenAccess W1509481275 @default.
- W1509481275 hasPrimaryLocation W15094812751 @default.
- W1509481275 hasRelatedWork W1477065389 @default.
- W1509481275 hasRelatedWork W1509481275 @default.
- W1509481275 hasRelatedWork W1648512565 @default.
- W1509481275 hasRelatedWork W1971828198 @default.
- W1509481275 hasRelatedWork W2000487610 @default.
- W1509481275 hasRelatedWork W2011312713 @default.
- W1509481275 hasRelatedWork W2023006608 @default.
- W1509481275 hasRelatedWork W2041190716 @default.
- W1509481275 hasRelatedWork W2061626952 @default.
- W1509481275 hasRelatedWork W2127980726 @default.
- W1509481275 hasVolume "128" @default.
- W1509481275 isParatext "false" @default.
- W1509481275 isRetracted "false" @default.
- W1509481275 magId "1509481275" @default.
- W1509481275 workType "article" @default.