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- W1509503169 abstract "A bifunctional probe (FecNC), containing a recognition part and an electrochemical active center, was applied to electrochemical detection of GG mismatch duplexes. The preparation of gold electrodes modified by mismatch and complementatry duplexes was characterized by electrochemical impedance spectroscopy (EIS) and optimized for better detection in terms of self-assembly time, hybridization time, and incubation time. The interaction between FecNC and DNA duplexes modified on the surface of a gold electrode was explored by square wave voltammetry (SWV) and EIS. The results showed that the DNA duplexes with GG mismatch on the surface of a gold electrode was easily detected by the largest electrochemical signal of the bifunctional probe because of its selective binding to GG mismatches. The bifunctional probe could offer a simple, effective electrochemical detection of GG mismatches, and theoretical bases for development of electrochemical biosensors. Further, the method would be favorable for diagnosis of genetic diseases." @default.
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- W1509503169 date "2015-07-01" @default.
- W1509503169 modified "2023-09-26" @default.
- W1509503169 title "Electrochemical Investigation of Interaction between a Bifunctional Probe and GG Mismatch Duplex" @default.
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- W1509503169 doi "https://doi.org/10.2116/analsci.31.663" @default.
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